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1. 发明公开

EP4361172A3 EXPRESSION VECTOR FOR RECOMBINANT U-CONOTOXIN TIIIA OR TIIIALAMUT EXPRESSION IN E.COLI 审中-公开
公开(公告)号：EP4361172A3
公开(公告)日：2024-05-29
申请号：EP23195429.8
申请日：2023-09-05
申请人： Science4Beauty Sp. z o.o.
发明人： Mazurkiewicz Pisarek, Anna , Mikiewicz, Diana , Mazurkiewicz, Alina , Ciach, Tomasz
IPC分类号： C07K14/435 , A61K38/00 , A61P29/00 , C12N9/02
CPC分类号： C07K14/43504 , A61K38/00 , A61P29/00 , C07K2319/0220130101 , C07K2319/2120130101 , C12N9/0036 , C07K2319/0020130101
摘要： Subject matter of the invention is a construct for an expression vector for recombinant µ-conotoxin TIIIA or TIIIAlaMut expression, characterized in that it contains either the nucleotide sequence of µ-conotoxin TIIIA SEQ ID NO: 1 or the nucleotide sequence of µ-conotoxin TIIIAlaMut SEQ ID NO: 4, both of which at the 5' end contain a sequence encoding six histidines (6His) linked by a serine-glycine-serine linker (SGS) to a construct encoding a TRX::TIIIA fusion protein containing the µ-conotoxin TIIIA gene and the gene encoding a leader protein, or a TRX::TIIIAlaMut fusion protein containing the µ-conotoxin TIIIAlaMut gene and a leader protein, where the said leader protein is thioredoxin (TRX) modified by point mutagenesis in which the amino acid methionine at position 37 has been replaced by lysine. Another subject matter of the invention is an expression vector containing the construct according to the invention under the control of a constitutive promoter. Another subject matter of the invention is an isolated E. coli cell containing the expression vector according to the invention. Another subject matter of the invention is a method for producing µ-conotoxin TIIIA or TIIIAlaMut in E. coli with the expression vector containing the construct according to the invention, characterized in that it includes the following steps: a) Transformation of an E. coli cell with the expression vector containing the construct according to the invention, under the control of a constitutive promoter, encoding either a TRX::TIIIA fusion protein with amino acid sequence SEQ ID NO: 2 or a TRX::TIIIAlaMut fusion protein with SEQ ID NO: 5; b) Expression of the TRX::TIIIA or TRX::TIIIAlaMut fusion protein; c) Isolation and purification of the TRX::TIIIA or TRX::TIIIAlaMut fusion protein; d) Disulfide bridges forming by subjecting the purified TRX::TIIIA or TRX:: TIIIAlaMut fusion protein to glutathione treatment in GSH/GSSG and dialysis in buffer; e) Cyanogen bromide cleavage of the TRX::TIIIA or TRX::TIIIAlaMut fusion protein with disulfide bridges formed; f) Purification of the cleaved TIIIA or TIIIAlaMut peptide.

2. 发明公开

EP4366763A1 CORONAVIRUS VACCINES 审中-公开
公开(公告)号：EP4366763A1
公开(公告)日：2024-05-15
申请号：EP22746995.4
申请日：2022-07-07
申请人： Luxembourg Institute of Health (LIH)
发明人： DERVILLEZ, Xavier , LÉONARD, Catherine , OLLERT, Markus, Wolfgang
IPC分类号： A61K39/12 , C07K14/78 , A61P31/14
CPC分类号： A61K39/12 , C12N2770/2003420130101 , A61P31/14 , C07K2319/7020130101 , C07K2319/73520130101 , C07K14/78 , C07K2319/2120130101

6. 发明授权

EP3592847B1 MMLV REVERSE TRANSCRIPTASE VARIANTS 审中-实审
公开(公告)号：EP3592847B1
公开(公告)日：2024-06-05
申请号：EP18791768.7
申请日：2018-04-26
IPC分类号： C12N9/12 , C12N9/22 , C12N15/33
CPC分类号： C12N9/1276 , C12Y207/07049 , C07K2319/2120130101 , C07K2319/5020130101

8. 发明公开

EP4356135A1 THERMOSTABLE AFFINITY POLYPEPTIDES 审中-公开
公开(公告)号：EP4356135A1
公开(公告)日：2024-04-24
申请号：EP22735381.0
申请日：2022-06-17
申请人： Roche Diagnostics GmbH , F. Hoffmann-La Roche AG
发明人： EISENHAUER, Romina , ENGEL, Alfred , JUCKNISCHKE, Ute , SCHRAEML, Michael , STEFFEN, Wojtek
IPC分类号： G01N33/543 , C07K14/00
CPC分类号： G01N33/54353 , G01N2333/24520130101 , C07K14/195 , C07K2319/0020130101 , C07K2319/2120130101

9. 发明公开

EP4377332A2 IL-10 INDUCING POLYPEPTIDES AND USES THEREOF 审中-公开
公开(公告)号：EP4377332A2
公开(公告)日：2024-06-05
申请号：EP22755185.0
申请日：2022-07-26
申请人： Enterome S.A.
发明人： BONNY, Christophe , CULTRONE, Antonietta , PFISTER, Hélène
IPC分类号： C07K14/54 , C07K14/24
CPC分类号： C07K14/5428 , C07K14/24 , A61K38/00 , A61P35/00 , C07K2319/0020130101 , C07K14/195 , C12R2001/1920210501 , C07K2319/2120130101 , C07K2319/0220130101 , C07K14/47 , A61P1/04 , Y02A50/30

10. 发明公开

EP4375376A2 MMLV REVERSE TRANSCRIPTASE VARIANTS 审中-公开
公开(公告)号：EP4375376A2
公开(公告)日：2024-05-29
申请号：EP24167618.8
申请日：2018-04-26
申请人： 10X Genomics, Inc.
发明人： LEE, Josephine , MARRS, Samuel , MCDERMOTT, Geoffrey , MESCHI, Francesca , MONTESCLAROS, Luz , PFEIFFER, Katherine , SHUGA, Joseph Francis , TERRY, Jessica Michele , ZIRALDO, Solongo B.
IPC分类号： C12N15/33
CPC分类号： C12N9/1276 , C12Y207/07049 , C07K2319/2120130101 , C07K2319/5020130101
摘要： Disclosed herein, are compositions, methods, and kits comprising engineered reverse transcription enzymes that exhibit several desired properties such as thermal stability, processive reverse transcription, non-templated base addition, and template switching ability. The engineered reverse transcription enzymes described herein demonstrate unexpectedly higher resistance to cell lysate inhibition, greater ability to capture full-length mRNA transcripts, and demonstrate improved results in small reaction volumes as compared to other engineered reverse transcription enzymes.

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