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    • 4. 发明公开
    • Haploid inducer
    • 单倍体诱导剂
    • EP3037540A1
    • 2016-06-29
    • EP14004389.4
    • 2014-12-23
    • KWS SAAT SE
    • BOLDUAN, Christof
    • C12N15/82C12N15/29C07K14/415A01H1/08
    • A01H1/08A01H1/02A01H5/06A01H5/10C07K14/415C12N15/8287
    • The present invention relates to non-transgenic and transgenic plants, preferably crop plants, having biological activity of a haploid inducer and comprising a polynucleotide which comprises a nucleotide sequence encoding a centromer histone H3 (CENH3) protein, wherein the polynucleotide comprises at least one mutation causing an alteration of the amino acid sequence of the CENH3 protein, and to a part of the part. Further, the invention provides methods of generating the inducer plants, methods of generating haploid and double haploid plants using the inducer plants as well as methods of facilitating cytoplasm exchange.
    • 本发明涉及具有单倍体诱导物的生物活性并且包含多核苷酸的非转基因和转基因植物,优选作物植物,所述多核苷酸包含编码中心体组蛋白H3(CENH3)蛋白的核苷酸序列,其中所述多核苷酸包含至少一个突变 引起CENH3蛋白质的氨基酸序列改变,并引起部分的一部分。 此外,本发明提供了产生诱导剂植物的方法,使用诱导剂植物产生单倍体和双倍体植物的方法以及促进细胞质交换的方法。