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1. 发明授权

EP1383915B1 METHOD FOR THE AMPLIFICATION AND DETECTION OF HBV DNA USING A TRANSCRIPTION BASED AMPLIFICATION 有权
标题翻译：基于转录的方法扩增和检测HBV DNA的
公开(公告)号：EP1383915B1
公开(公告)日：2015-11-18
申请号：EP02702404.1
申请日：2002-03-06
申请人： bioMerieux B.V
发明人： DEIMAN, Birgit, Alberta, Louisa, Maria , FRANTZEN, Inge, Marjolein , STRIJP, Arnoldina, Margaretha, Wilhelmina
IPC分类号： C12Q1/68
CPC分类号： C12Q1/706 , C12Q2531/143

2. 发明公开

EP1383915A2 METHOD FOR THE AMPLIFICATION AND DETECTION OF HBV DNA USING A TRANSCRIPTION BASED AMPLIFICATION 有权
标题翻译：基于转录的方法扩增和检测HBV DNA的
公开(公告)号：EP1383915A2
公开(公告)日：2004-01-28
申请号：EP02702404.1
申请日：2002-03-06
申请人： bioMerieux B.V
发明人： DEIMAN, Birgit, Alberta, Louisa, Maria , FRANTZEN, Inge, Marjolein , STRIJP, Arnoldina, Margaretha, Wilhelmina
IPC分类号： C12Q1/68
CPC分类号： C12Q1/706 , C12Q2531/143
摘要： The present invention provides a method for the transcription based amplification of a target HBV nucleic acid sequence starting from HBV DNA optionally present in a sample, comprising the steps of, - incubating the sample, suspected to contain HBV, in an amplification buffer with one or more restriction enzymes capable of cleaving the HBV DNA at a selected restriction site, said restriction enzyme creating a defined 3' end of the said HBV DNA strand(s), a promotor-primer, said promotor-primer having a 5' region comprising the sequence of a promotor recognized by a DNA-dependent RNA polymerase and a 3' region complementary to the define 3' end of the DNA strand, a second or reverse primer, having the opposite polarity of the promotor-primer and comprising the 5' end of the said target sequence, and in case of HBV ssDNA as the target sequence, a restriction primer, - maintaining the thus created reaction mixture under the appropriate conditions for a sufficient amount of time for a digestion by the restriction enzyme to take place, - subjecting the sample thus obtained to a heat treatment at a temperature and time sufficient to inactivate the restriction enzyme and/or to render at least partially a double strand single stranded, - adding the following reagents to the sample: an enzyme having RNA dependent DNA polymerase activity, an enzyme having DNA dependent DNA polymerase activity, an enzyme having Rnase H activity, an enzyme having RNA polymerase activity, and - maintaining the thus created reaction mixture under the appropriate conditions to a sufficient amount of time for the amplification to take place.

3. 发明公开

EP1682676A1 METHOD FOR AMPLIFICATION OF RNA SEQUENCES 有权
标题翻译： RNA序列的方法扩增
公开(公告)号：EP1682676A1
公开(公告)日：2006-07-26
申请号：EP04790962.7
申请日：2004-10-27
申请人： bioMerieux B.V.
发明人： DEIMAN, Birgit, Alberta, Louisa, Maria , STRIJP, Arnoldina, Margaretha, Wilhelmina
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6865 , C12Q2525/179 , C12Q2525/143
摘要： The invention relates to a method for amplification of a target RNA sequence, wherein the first primer comprises a hybridizing sequence of 7 to 14 nucleotides, which is capable of binding to a first segment of the target RNA sequence, a transcription enhancing sequence, and an anchor which is capable of binding to a second segment of the target RNA sequence, and/or wherein the second primer comprises a hybridizing sequence of 7 to 14 nucleotides, an amplification enhancing sequence and an anchor which is capable of binding to a second segment of the first single stranded cDNA. The invention further relates to primers for the amplification of target RNA sequences and to a kit comprising one or more of the primers.

4. 发明授权

EP1682676B1 METHOD FOR AMPLIFICATION OF RNA SEQUENCES 有权
标题翻译： RNA序列的方法扩增
公开(公告)号：EP1682676B1
公开(公告)日：2011-12-28
申请号：EP04790962.7
申请日：2004-10-27
申请人： bioMerieux B.V.
发明人： DEIMAN, Birgit, Alberta, Louisa, Maria , STRIJP, Arnoldina, Margaretha, Wilhelmina
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6865 , C12Q2525/179 , C12Q2525/143
摘要： The invention relates to a method for amplification of a target RNA sequence, wherein the first primer comprises a hybridizing sequence of 7 to 14 nucleotides, which is capable of binding to a first segment of the target RNA sequence, a transcription enhancing sequence, and an anchor which is capable of binding to a second segment of the target RNA sequence, and/or wherein the second primer comprises a hybridizing sequence of 7 to 14 nucleotides, an amplification enhancing sequence and an anchor which is capable of binding to a second segment of the first single stranded cDNA. The invention further relates to primers for the amplification of target RNA sequences and to a kit comprising one or more of the primers.

5. 发明公开

EP1366179A2 METHOD FOR THE AMPLIFICATION AND DETECTION OF DNA USING A TRANSCRIPTION BASED AMPLIFICATION 审中-公开
标题翻译：方法用于扩增和DNA USING A转录检测基于功放
公开(公告)号：EP1366179A2
公开(公告)日：2003-12-03
申请号：EP02727384.6
申请日：2002-03-05
申请人： bioMerieux B.V
发明人： DEIMAN, Birgit, Alberta, Louisa, Maria , FRANTZEN, Inge, Marjolein , STRIJP, Arnoldina, Margaretha, Wilhelmina
IPC分类号： C12Q1/18
CPC分类号： C12Q1/6865 , C12Q2521/301 , C12Q2521/107
摘要： The present invention is directed to a transcription based amplification method for the amplification of DNA targets starting from ds or ssDNA optionally present in a sample, comprising the steps of: - incubating the sample in an amplification buffer with one or more restriction enzymes capable of cleaving DNA at a selected restriction site, said restriction enzyme creating a defined 3' end on the said DNA strand(s), and a promoter-primer, said promoter-primer having a 5' region comprising the sequence of a promoter recognized by a DNA-dependent RNA polymerase and a 3' region complementary to the defined 3' end of the DNA strand, a second primer, having the opposite polarity of the promoter-primer and comprising the 5' end of the target sequence, and in case of ssDNA as the target DNA, a restriction primer; - maintaining the thus created reaction mixture under the appropriate conditions for a sufficient amount of time for a digestion by the restriction enzyme to take place; - subjecting the sample to a heat treatment at a temperature and time sufficient to inactivate the restricting enzyme and/or to render a double strand single stranded; - adding the following reagents to the sample: an enzyme having RNA dependent DNA polymerase activity, an enzyme having DNA dependent DNA polymerase activity, an enzyme having Rnase H activity, an enzyme having RNA polymerase activity; and - maintaining the thus created reaction mixture under the appropriate conditions for a sufficient amount of time for the amplification to take place.

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