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1. 发明公开

EP3472132A1 FLUORESCENCE TAGGING OF GLYCANS AND OTHER BIOMOLECULES THROUGH REDUCTIVE AMINATION FOR ENHANCED MS SIGNALS 无效
公开(公告)号：EP3472132A1
公开(公告)日：2019-04-24
申请号：EP17815987.7
申请日：2017-06-19
申请人： Waters Technologies Corporation
发明人： BROUSMICHE, Darryl, W. , LAUBER, Matthew, A.
IPC分类号： C07D215/16 , C07D215/38 , C07D215/48 , G01N33/533

3. 发明授权

EP3213056B1 METHODS FOR THE RAPID PREPARATION OF LABELED GLYCOSYLAMINES AND FOR THE ANALYSIS OF GLYCOSYLATED BIOMOLECULES PRODUCING THE SAME 无效转让
公开(公告)号：EP3213056B1
公开(公告)日：2020-08-12
申请号：EP15855907.0
申请日：2015-10-28
申请人： Waters Technologies Corporation
发明人： LAUBER, Matthew, A. , BROUSMICHE, Darryl, W. , KOZA, Stephan, M.
IPC分类号： G01N21/76 , G01N33/58 , G01N33/68

4. 发明公开

EP3519832A1 LABELED GLYCAN AMINO ACID COMPLEXES USEFUL IN LC-MS ANALYSIS AND METHODS OF MAKING THE SAME 有权
公开(公告)号：EP3519832A1
公开(公告)日：2019-08-07
申请号：EP17800637.5
申请日：2017-09-29
申请人： Waters Technologies Corporation
发明人： RAINVILLE, Paul , LAUBER, Matthew, A. , GETHINGS, Lee , PLUMB, Robert, S. , COSGRAVE, Eoin , BROUSMICHE, Darryl, W.
IPC分类号： G01N33/68

5. 发明公开

EP4213985A1 SILICA-BASED PARTICLE WITH HYDROXY-TERMINATED PEG BONDING AND METHOXY-TERMINATED PEG SURFACE MODIFICATION 审中-实审
公开(公告)号：EP4213985A1
公开(公告)日：2023-07-26
申请号：EP21810457.8
申请日：2021-09-16
申请人： Waters Technologies Corporation
发明人： LAWRENCE, Nicole, L. , TUNC SARISOZEN, Yeliz , BROUSMICHE, Darryl, W. , LAUBER, Matthew, A.
IPC分类号： B01J20/28 , B01J20/286 , B01J20/32 , B01D15/20 , B01D15/34

6. 发明公开

EP3471728A1 METHODS OF ENHANCING MS DETECTION OF TAGGED GLYCANS 审中-实审
公开(公告)号：EP3471728A1
公开(公告)日：2019-04-24
申请号：EP17816001.6
申请日：2017-06-19
申请人： Waters Technologies Corporation
发明人： BROUSMICHE, Darryl, W. , LAUBER, Matthew, A.
IPC分类号： A61K31/4709 , G01N33/50 , G01N33/58

7. 发明公开

EP3213056A1 METHODS FOR THE RAPID PREPARATION OF LABELED GLYCOSYLAMINES AND FOR THE ANALYSIS OF GLYCOSYLATED BIOMOLECULES PRODUCING THE SAME 无效转让
标题翻译：快速制备标记的甘氨酰胺和分析生成相同生物分子的糖基化物的方法
公开(公告)号：EP3213056A1
公开(公告)日：2017-09-06
申请号：EP15855907.0
申请日：2015-10-28
申请人： Waters Technologies Corporation
发明人： LAUBER, Matthew, A. , BROUSMICHE, Darryl, W. , KOZA, Stephan, M.
IPC分类号： G01N21/76
摘要： Methods of analyzing glycosylated biomolecules include the steps of producing a deglycosylation mixture of biomolecules deglycosylated by natural or synthetic enzymatic or chemical techniques; providing a reagent solution having a labeling reagent in a polar aprotic, non-nucleophilic organic solvent; and mixing the deglycosylation mixture with the reagent solution in an excess of labeling reagent to produce derivatized glycosylamines. The method steps can be carried out purposefully without depletion of protein matter. A quenching solution can be added to the reaction mixture so that the pH of the reaction mixture is shifted to above 10. The yield of derivatized glycosylamines can be in an amount of about 80 to about 100 mole percent of the reaction mixture with minimal overlabeling, less than 0.2 mole percent. The derivizated glycosylamines can be separated from the reaction mixture and detected by chromatographic detection, fluorescence detection, mass spectrometry (“MS”), or Ultra Violet (“UV”) detection and/or a combination thereof.
摘要翻译：分析糖基化生物分子的方法包括以下步骤：产生通过天然或合成酶或化学技术去糖基化的生物分子的去糖基化混合物; 提供在极性非质子非亲核有机溶剂中具有标记试剂的试剂溶液; 并将去糖基化混合物与试剂溶液在过量的标记试剂中混合以产生衍生的糖胺。该方法步骤可以有目的地进行而不消耗蛋白质物质。可以将淬灭溶液加入到反应混合物中，使得反应混合物的pH移至10以上。衍生的糖胺的产量可以为反应混合物的约80至约100摩尔％，具有最小的重叠标记，小于0.2摩尔％。衍生的糖胺可以从反应混合物中分离并通过色谱检测，荧光检测，质谱（“MS”）或紫外（“UV”）检测和/或其组合检测。

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