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1. 发明授权

EP2828218B1 METHODS OF LOWERING THE ERROR RATE OF MASSIVELY PARALLEL DNA SEQUENCING USING DUPLEX CONSENSUS SEQUENCING 有权
公开(公告)号：EP2828218B1
公开(公告)日：2020-08-05
申请号：EP13764186.6
申请日：2013-03-15
申请人： University of Washington through its Center for Commercialization
发明人： SCHMITT, Michael , SALK, Jesse , LOEB, Lawrence, A.
IPC分类号： C12Q1/6876 , C12Q1/6869 , C12Q1/6806

2. 发明公开

EP4234713A3 METHODS OF LOWERING THE ERROR RATE OF MASSIVELY PARALLEL DNA SEQUENCING USING DUPLEX CONSENSUS SEQUENCING 审中-实审
公开(公告)号：EP4234713A3
公开(公告)日：2023-10-25
申请号：EP23178659.1
申请日：2013-03-15
申请人： University of Washington through its Center for Commercialization
发明人： SALK, Jesse , LOEB, Lawrence, A. , SCHMITT, Michael
IPC分类号： C04B20/04 , C12Q1/6806 , C12Q1/6869
摘要： Next Generation DNA sequencing promises to revolutionize clinical medicine and basic research. However, while this technology has the capacity to generate hundreds of billions of nucleotides of DNA sequence in a single experiment, the error rate of approximately 1% results in hundreds of millions of sequencing mistakes. These scattered errors can be tolerated in some applications but become extremely problematic when "deep sequencing" genetically heterogeneous mixtures, such as tumors or mixed microbial populations. To overcome limitations in sequencing accuracy, a method Duplex Consensus Sequencing (DCS) is provided. This approach greatly reduces errors by independently tagging and sequencing each of the two strands of a DNA duplex. As the two strands are complementary, true mutations are found at the same position in both strands. In contrast, PCR or sequencing errors will result in errors in only one strand.

3. 发明公开

EP4234713A2 METHODS OF LOWERING THE ERROR RATE OF MASSIVELY PARALLEL DNA SEQUENCING USING DUPLEX CONSENSUS SEQUENCING 审中-实审
公开(公告)号：EP4234713A2
公开(公告)日：2023-08-30
申请号：EP23178659.1
申请日：2013-03-15
申请人： University of Washington through its Center for Commercialization
发明人： SALK, Jesse , LOEB, Lawrence, A. , SCHMITT, Michael
IPC分类号： C12Q1/6806
摘要： Next Generation DNA sequencing promises to revolutionize clinical medicine and basic research. However, while this technology has the capacity to generate hundreds of billions of nucleotides of DNA sequence in a single experiment, the error rate of approximately 1% results in hundreds of millions of sequencing mistakes. These scattered errors can be tolerated in some applications but become extremely problematic when "deep sequencing" genetically heterogeneous mixtures, such as tumors or mixed microbial populations. To overcome limitations in sequencing accuracy, a method Duplex Consensus Sequencing (DCS) is provided. This approach greatly reduces errors by independently tagging and sequencing each of the two strands of a DNA duplex. As the two strands are complementary, true mutations are found at the same position in both strands. In contrast, PCR or sequencing errors will result in errors in only one strand.

4. 发明公开

EP2828218A1 METHODS OF LOWERING THE ERROR RATE OF MASSIVELY PARALLEL DNA SEQUENCING USING DUPLEX CONSENSUS SEQUENCING 有权
标题翻译：用双重约定序列降低质量平行DNA序列误差率的方法
公开(公告)号：EP2828218A1
公开(公告)日：2015-01-28
申请号：EP13764186.6
申请日：2013-03-15
申请人： University of Washington through its Center for Commercialization
发明人： SCHMITT, Michael , SALK, Jesse , LOEB, Lawrence, A.
IPC分类号： C04B20/04
摘要： Next Generation DNA sequencing promises to revolutionize clinical medicine and basic research. However, while this technology has the capacity to generate hundreds of billions of nucleotides of DNA sequence in a single experiment, the error rate of approximately 1% results in hundreds of millions of sequencing mistakes. These scattered errors can be tolerated in some applications but become extremely problematic when “deep sequencing” genetically heterogeneous mixtures, such as tumors or mixed microbial populations. To overcome limitations in sequencing accuracy, a method Duplex Consensus Sequencing (DCS) is provided. This approach greatly reduces errors by independently tagging and sequencing each of the two strands of a DNA duplex. As the two strands are complementary, true mutations are found at the same position in both strands. In contrast, PCR or sequencing errors will result in errors in only one strand. This method uniquely capitalizes on the redundant information stored in double-stranded DNA, thus overcoming technical limitations of prior methods utilizing data from only one of the two strands.
摘要翻译：下一代DNA测序有望彻底改变临床医学和基础研究。然而，尽管该技术能够在单个实验中产生数千亿个DNA序列的核苷酸，但大约1％的错误率导致了数亿个测序错误。这些分散的错误在某些应用中是可以容忍的，但在对基因异质混合物（如肿瘤或混合微生物群体）进行“深度测序”时会变得非常成问题。为了克服测序准确性的限制，提供了双重共有序列（DCS）方法。这种方法通过独立地标记和测序DNA双链体的两条链中的每一条来大大减少错误。由于两条链是互补的，所以在两条链中的相同位置都发现了真正的突变。相反，PCR或测序错误只会在一条链中产生错误。

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