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2. 发明公开

EP1646721A1 A METHOD OF SELECTIVELY PRODUCING MALE OR FEMALE STERILE PLANTS 有权
标题翻译：一种选择性生产雄性或雌性无性系植物的方法
公开(公告)号：EP1646721A1
公开(公告)日：2006-04-19
申请号：EP04736419.5
申请日：2004-06-09
申请人： Syngenta Limited
发明人： DALE, Richard , HAWKES, Timothy Robert , THOMPSON, Paul Anthony , VINER, Russell
IPC分类号： C12N15/82 , C12N9/06
CPC分类号： C12N9/0022 , C12N15/8287
摘要： A method of producing male or female sterile plants comprising the steps of transforming plant material with a polynucleotide which encodes at least one enzyme which reacts with a non-phytotoxic substance to produce a phytotoxic one, and regenerating the thus transformed material into a plant, wherein the said non-phytotoxic substance is applied to the plant up to the time of male or female gamete formation and/ or maturation, so that the non-phytotoxic substance provides for the production of a phytotoxic one which selectively prevents the formation of or otherwise renders the said gametes non-functional, wherein the enzyme is expressed preferentially in either male, or female reproductive structures and the non-phytotoxic substance is a D-alpha amino acid, or a peptide derivative of a non-protein D-alpha amino acid, characterised in that the enzyme is a mutant D-amino acid oxidase, obtainable from Rhodotorula gracilis, which oxidase comprises a lysine at position 58 rather than a phenylalanine in the wild type sequence.
摘要翻译：一种生产雄性或雌性不育植物的方法，包括用编码至少一种酶的多核苷酸转化植物材料的步骤，所述多核苷酸与非植物毒性物质反应产生植物毒性物质，并将如此转化的物质再生成植物，其中所述非植物毒性物质施用于植物直至雄性或雌性配子形成和/或成熟时，以使非植物毒性物质提供植物毒性物质的产生，所述植物毒性物质选择性地防止形成或以其他方式呈现所述配子是非功能性的，其中所述酶优先在雄性或雌性生殖结构中表达，并且所述非植物毒性物质是D-α氨基酸或非蛋白质D-α氨基酸的肽衍生物，其特征在于所述酶是可从细胞红酵母获得的突变型D-氨基酸氧化酶，所述氧化酶包含位置58处的赖氨酸而不是phe 野生型序列中的丙氨酸。

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