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    • 1. 发明公开
    • Method and kit for positively detecting toxic material
    • Verfahren und Kit zum肯定了Nachweis von giftigen Materialien
    • EP1811298A1
    • 2007-07-25
    • EP06124019.8
    • 2006-11-14
    • Samsung Electronics Co., Ltd.
    • Han, Jung-imChoi, Soo-hyungLee, Soo-sukJung, Sung-oukPark, Joon-shikKim, Ki-eun
    • G01N33/50
    • G01N33/5079G01N2333/90209
    • Provided are a method for positively detecting toxic materials within a sample including (i) contacting sub-mitochondria particles having competent mitochondrial enzymes derived from the inner membranes of mitochondria, and an electron donor which transmits electrons to an electron transfer system of the sub-mitochondria particles, with a sample to be tested, (ii) adjusting the pH of one or more pH indicators to a pH higher than the pH of the sample reaction of (i), (iii) adding the pH indicator(s) of (ii) to each sample reaction of (i), and (iv) identifying color changes of one or more pH indicators. A kit for performing said method is also provided.
      All kinds of toxic materials can be detected as well as the presence of toxic materials and their degree of toxicity. In addition, no special equipment or apparatus is required and thus, simplification of the method is possible. When materials desired to be detected exist, the method of positively detecting toxic materials within a sample is the same as a general positive detection mechanism and thus, commercialization is also possible.
    • 提供一种用于积极检测样品中的有毒物质的方法,包括(i)接触源自线粒体内膜的感受态线粒体酶的亚线粒体颗粒和将电子传递到子线粒体的电子转移系统的电子供体 (ii)将一个或多个pH指示剂的pH调节到比(i)的样品反应的pH高的pH,(iii)加入(ii)的pH指示剂 )到(i)的每个样品反应,和(iv)鉴定一个或多个pH指示剂的颜色变化。 还提供了用于执行所述方法的套件。 可以检测各种有毒物质,以及有毒物质的存在及其毒性程度。 此外,不需要特殊的设备或装置,因此可以简化方法。 当需要检测的材料存在时,正面检测样品中的有毒物质的方法与普通的阳性检测机制相同,因此商业化也是可能的。