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1. 发明公开

EP0856253A3 Mass production and long-term preservation of fungivorous nematodes and used thereof 失效
标题翻译：大规模生产和真菌线虫及其应用的长期保存
公开(公告)号：EP0856253A3
公开(公告)日：1999-04-28
申请号：EP98300463.1
申请日：1998-01-23
申请人： SAGA UNIVERSITY
发明人： Ishibashi, Nobuyoshi
IPC分类号： A01K67/033
CPC分类号： A01K67/033
摘要： A host fungus of a fungivorous nematode is inoculated on a solid medium or an artificial liquid medium containing an industrial vegetable waste or byproduct, and then the nematode whose whole body have been sterilized is inoculated and mass-cu ltivated. Fungivorous ability of the nematode can be kept by subculturing using different host fungus on every culturing stage. The nematodes, when maintained about 10 days in an aerobic condition at 20-25°C with a relative humidity gradually inclined from high to low and dried to anhydrobiotic conditions, can be preserved for a long time. The nematode can be used for biological control of soil pathogens and soil insect pests.

2. 发明公开

EP0856253A2 Mass production and long-term preservation of fungivorous nematodes and used thereof 失效
标题翻译：马萨诸塞州和安格尔登
公开(公告)号：EP0856253A2
公开(公告)日：1998-08-05
申请号：EP98300463.1
申请日：1998-01-23
申请人： SAGA UNIVERSITY
发明人： Ishibashi, Nobuyoshi
IPC分类号： A01K67/033
CPC分类号： A01K67/033
摘要： A host fungus of a fungivorous nematode is inoculated on a solid medium or an artificial liquid medium containing an industrial vegetable waste or byproduct, and then the nematode whose whole body have been sterilized is inoculated and mass-cu ltivated. Fungivorous ability of the nematode can be kept by subculturing using different host fungus on every culturing stage. The nematodes, when maintained about 10 days in an aerobic condition at 20-25°C with a relative humidity gradually inclined from high to low and dried to anhydrobiotic conditions, can be preserved for a long time. The nematode can be used for biological control of soil pathogens and soil insect pests.
摘要翻译：将真菌线虫的宿主真菌接种在固体培养基或含有工业蔬菜废弃物或副产物的人造液体培养基上，然后将全身灭菌的线虫接种并进行大量培养。通过在每个培养阶段使用不同的宿主真菌进行传代培养可以保持线虫的活力。当在20-25℃的有氧条件下保持约10天，相对湿度从高到低逐渐倾斜并干燥至无水生物条件时，线虫可以长时间保存。线虫可用于土壤病原体和土壤虫害的生物防治。

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