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1. 发明公开

EP1029077A2 SPEZIFISCHES UND EMPFINDLICHES NUKLEINSÄURENACHWEISVERFAHREN 审中-公开
标题翻译：特异性和敏感性NUCLEIC检测方法
公开(公告)号：EP1029077A2
公开(公告)日：2000-08-23
申请号：EP98955529.7
申请日：1998-11-03
申请人： Roche Diagnostics GmbH
发明人： KESSLER, Christoph , HABERHAUSEN, Gerd , BARTL, Knut , ORUM, Henrik
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6823 , C12Q1/6818 , C12Q1/686 , C12Q2561/101
摘要： The invention relates to a method for detecting a nucleic acid comprising the production of a plurality of amplifications of a section of said nucleic acid with the assistance of two primers of which one can bond on a bonding sequence A of the nucleic acid and the other can bond on a bonding sequence C' which is complimentary to a sequence C. Sequence C does not overlap A and is situated in a 3' direction from A. The inventive method also includes bringing the amplifications in contact with a probe having a bonding sequence D which can bond on a sequence B, said sequence B being situated between sequences A and C, or the complement thereof. In addition, the invention relates to the detection of the construction of a hybrid out of the amplification and the probe, whereby the sequence situated between the bonding sequences A and C contains no nucleotides, said nucleotides not being linked to the bonding sequence D of the probe or to complement D' thereof.

2. 发明授权

EP1466018B1 USE OF SILICA MATERIAL IN AN AMPLIFICATION REACTION 无效转让
标题翻译： USING A二氧化硅材料WHEN扩增
公开(公告)号：EP1466018B1
公开(公告)日：2007-10-03
申请号：EP03729220.8
申请日：2003-01-04
申请人： Roche Diagnostics GmbH , F.HOFFMANN-LA ROCHE AG
发明人： PINSL-OBER, Judith , WENZIG, Peter , WEINDEL, Kurt , BARTL, Knut , SCHOENBRUNNER, Ralf , MALHOTRA, Khushbeer , O'DONNELL, Patrick , KYGER, Erich
IPC分类号： C12Q1/68 , C12N15/10
CPC分类号： C12N15/1006 , C12Q1/6806 , C12Q2547/101 , C12Q2531/113
摘要： This invention relates to a method for the isolation of a target nucleic acid using a material with an unmodified silica surface and subsequent amplification of the target nucleic acid in the presence of the material with an unmodified silica surface. The method is preferably carried out as an automated process preferably in a high throughput format. The method is preferably used in diagnostics.

3. 发明公开

EP1029084A2 SPEZIFISCHES UND SENSITIVES NUKLEINSÄURENACHWEISVERFAHREN 审中-公开
标题翻译：特异性和敏感性NUCLEIC检测方法
公开(公告)号：EP1029084A2
公开(公告)日：2000-08-23
申请号：EP98965653.3
申请日：1998-11-03
申请人： Roche Diagnostics GmbH
发明人： KESSLER, Christoph , HABERHAUSEN, Gerd , BARTL, Knut , ORUM, Henrik
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6823 , C12Q1/6818 , C12Q1/686 , C12Q2561/101
摘要： The invention relates to a method for detecting a nucleic acid, comprising the following steps: the production of a number of enhancers of a fragment of said nucleic acid with a length of less than 100 nucleotides using two primers, one of said primers being able to bind to a first binding sequence (A) of a strand of the nucleic acid and the other primer being able to bind to a second binding sequence (C') which is essentially complementary to a sequence (C), said sequence (C) not overlapping with (A) and being positioned in the direction 3' from A, in the presence of a probe with a binding sequence (D) which is able to bind to the third sequence (B) positioned between the sequences (A) and (C) or the complement (B') thereof, said probe containing a reporter group and a quencher group, using a polymerase with 5' nuclease activity; and detection of the nucleic acid through the measurement of a signal which is determined by the release of the reporter group.

4. 发明公开

EP1029083A2 SPEZIFISCHES UND SENSITIVES NUKLEINSÄURENACHWEISVERFAHREN 审中-公开
标题翻译： SPEZIFISCHES UND SENSITIVESNUKLEINSÄURENACHWEISVERFAHREN
公开(公告)号：EP1029083A2
公开(公告)日：2000-08-23
申请号：EP98965652.5
申请日：1998-11-03
申请人： Roche Diagnostics GmbH
发明人： KESSLER, Christoph , HABERHAUSEN, Gerd , BARTL, Knut , ORUM, Henrik
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6823 , C12Q1/6818 , C12Q1/686 , C12Q2561/101
摘要： The invention relates to a method for detecting a nucleic acid in which a plurality of amplificates of a section of the nucleic acid is produced with the aid of two primers. One primer can bond on a bonding sequence A of the nucleic acid, and the other can bond on a bonding sequence C' which is complimentary to a sequence C, does not overlap with A, and is placed in a 3'-direction of A. The amplificates are brought into contact with a probe having a bonding sequence D which can bond on a sequence B or the complement thereof, said sequence B and the complement thereof being placed between sequences A and C. The formation of a hybrid out of the amplificate and the probe is detected, whereby the sequence placed between bonding sequences A and C contains no nucleotides which do not belong to the bonding sequence D or complement D' thereof of the probe.
摘要翻译：本发明涉及一种检测核酸的方法，其中借助于两种引物生成多个核酸片段的扩增片段。一个引物可以结合在核酸的结合序列A上，另一个可以结合在与序列C互补的结合序列C'上，不与A重叠，并且位于A'的3'方向上使扩增产物与具有能结合在序列B或其互补序列上的结合序列D的探针接触，所述序列B及其互补序列位于序列A和C之间。形成杂交体扩增并检测探针，由此位于结合序列A和C之间的序列不含不属于探针的结合序列D或其补体D'的核苷酸。

5. 发明授权

EP1466018B2 USE OF SILICA MATERIAL IN AN AMPLIFICATION REACTION 无效转让
标题翻译： USING A二氧化硅材料WHEN扩增
公开(公告)号：EP1466018B2
公开(公告)日：2015-08-12
申请号：EP03729220.8
申请日：2003-01-04
申请人： Roche Diagnostics GmbH , F.Hoffmann-La Roche AG
发明人： PINSL-OBER, Judith , WENZIG, Peter , WEINDEL, Kurt , BARTL, Knut , SCHOENBRUNNER, Ralf , MALHOTRA, Khushbeer , O'DONNELL, Patrick , KYGER, Erich
IPC分类号： C12Q1/68 , C12N15/10
CPC分类号： C12N15/1006 , C12Q1/6806 , C12Q2547/101 , C12Q2531/113

6. 发明公开

EP1466018A2 USE OF SILICA MATERIAL IN AN AMPLIFICATION REACTION 无效转让
标题翻译： USING A二氧化硅材料WHEN扩增
公开(公告)号：EP1466018A2
公开(公告)日：2004-10-13
申请号：EP03729220.8
申请日：2003-01-04
申请人： Roche Diagnostics GmbH , F.HOFFMANN-LA ROCHE AG
发明人： PINSL-OBER, Judith , WENZIG, Peter , WEINDEL, Kurt , BARTL, Knut , SCHOENBRUNNER, Ralf , MALHOTRA, Khushbeer , O'DONNELL, Patrick , KYGER, Erich
IPC分类号： C12Q1/68 , C12N15/10
CPC分类号： C12N15/1006 , C12Q1/6806 , C12Q2547/101 , C12Q2531/113
摘要： This invention relates to a method for the isolation of a target nucleic acid using a material with an unmodified silica surface and subsequent amplification of the target nucleic acid in the presence of the material with an unmodified silica surface. The method is preferably carried out as an automated process preferably in a high throughput format. The method is preferably used in diagnostics.

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