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1. 发明公开

EP2366779A1 Mutant luciferase having increased thermostability 有权
公开(公告)号：EP2366779A1
公开(公告)日：2011-09-21
申请号：EP10179372.7
申请日：1999-10-26
申请人： Promega Corporation
发明人： Squirrell, David James , Murphy, Melanie Jane , Leslie, Rachel Louise , Lowe, Christopher Robin , White, Peter John , Tisi, Laurence Carlo , Murray, James Augustus Henry
IPC分类号： C12N9/02 , A01H4/00
CPC分类号： C12N9/0069 , C12Q1/66
摘要： A protein having luciferase activity and at least 60% similarity to luciferase from Photinus pyralis, Luciola mingrelica, Luciola cruciata or Luciola lateralis, Hotaria paroula, Pyrophorus plagiophthalamus, Lampyris noctiluca, Pyrocoelia nayako or Photinus pennsylanvanica, wherein in the sequence of the enzyme, at least one of
(a) the amino acid residue corresponding to residue 214 in Photinus pyralis luciferase;
(b) the amino acid residue corresponding to residue 232 in Photinus pyralis luciferase;
(c) the amino acid residue corresponding to residue 295 in Photinus pyralis luciferase;
(d) the amino acid residue corresponding to amino acid 14 of the Photinus pyralis luciferase;
(e) the amino acid residue corresponding to amino acid 35 of the Photinus pyralis luciferase;
(f) the amino acid residue corresponding to amino acid residue 105 of the Photinus pyralis luciferase;
(g) the amino acid residue corresponding to amino acid residue 234 of the Photinus pyralis luciferase;
(h) the amino acid residue corresponding to amino acid residue 420 of the Photinus pyralis luciferase;
(i) the amino acid residue corresponding to amino acid residue 310 of the Photinus pyralis luciferase;
is different to the amino acid which appears in the corresponding wild type sequence and wherein the luciferase enzyme has increased thermostability as compared to an enzyme having the amino acid of the corresponding wild-type luciferase at this position.

2. 发明授权

EP2071023B1 Novel enzyme 有权
标题翻译：新型酶
公开(公告)号：EP2071023B1
公开(公告)日：2016-04-27
申请号：EP08013254.1
申请日：1999-10-26
申请人： Promega Corporation
发明人： Squirrell, David James , Murphy, Melenie Jane , Leslie, Rachel Louise , Lowe, Christopher Robin , White, Peter John , Tisi, Laurence Carlo , Murray, James Augustus Henry
IPC分类号： C12N9/02 , A01H4/00
CPC分类号： C12N9/0069 , C12Q1/66

3. 发明公开

EP2366778A1 Mutant luciferase having increased thermostability 有权
标题翻译：突变型萤光素酶具有增加的热稳定性
公开(公告)号：EP2366778A1
公开(公告)日：2011-09-21
申请号：EP10179359.4
申请日：1999-10-26
申请人： Promega Corporation
发明人： Squirrell, David James , Murphy, Melanie Jane , Leslie, Rachel Louise , Lowe, Christopher Robin , White, Peter John , Tisi, Laurence Carlo , Murray, James Augustus Henry
IPC分类号： C12N9/02 , A01H4/00
CPC分类号： C12N9/0069 , C12Q1/66
摘要： A protein having luciferase activity and at least 60% similarity to luciferase from Photinus pyralis, Luciola mingrelica, Luciola cruciata or Luciola lateralis, Hotaria paroula, P yrophorus plagiophthalamus, Lampyris noctiluca, Pyrocoelia nayako or Photinus pennsylanvanica, wherein in the sequence of the enzyme, at least one of
(a) the amino acid residue corresponding to residue 214 in Photinus pyralis luciferase;
(b) the amino acid residue corresponding to residue 232 in Photinus pyralis luciferase;
(c) the amino acid residue corresponding to residue 295 in Photinus pyralis luciferase;
(d) the amino acid residue corresponding to amino acid 14 of the Photinus pyralis luciferase;
(e) the amino acid residue corresponding to amino acid 35 of the Photinus pyralis luciferase;
(f) the amino acid residue corresponding to amino acid residue 105 of the Photinus pyralis luciferase;
(g) the amino acid residue corresponding to amino acid residue 234 of the Photinus pyralis luciferase;
(h) the amino acid residue corresponding to amino acid residue 420 of the Photinus pyralis luciferase;
(i) the amino acid residue corresponding to amino acid residue 310 of the Photinus pyralis luciferase;

is different to the amino acid which appears in the corresponding wild type sequence and wherein the luciferase enzyme has increased thermostability as compared to an enzyme having the amino acid of the corresponding wild-type luciferase at this position.
摘要翻译：一种具有荧光素酶活性并与来自Photinus pyralis，Luciola mingrelica，Cruciata或Luciola lateralis，Hotaria paroula，Pyrophorus plagiophthalamus，Lampyris noctiluca，Pyrocoelia nayako或Photinus pennsylanvanica的萤光素酶具有至少60％相似性的蛋白质，其中在所述酶的序列中，（a）对应于Photinus pyralis萤光素酶中残基214的氨基酸残基中的至少一个; （b）对应于Photinus pyralis萤光素酶中残基232的氨基酸残基; （c）对应于Photinus pyralis萤光素酶中残基295的氨基酸残基; （d）与Photinus pyralis萤光素酶的氨基酸14对应的氨基酸残基; （e）与Photinus pyralis萤光素酶的第35位氨基酸对应的氨基酸残基; （f）对应于萤火虫萤光素酶的氨基酸残基105的氨基酸残基; （g）与Photinus pyralis萤光素酶的氨基酸残基234相对应的氨基酸残基; （h）与Photinus pyralis萤光素酶的氨基酸残基420相对应的氨基酸残基; （i）与Photinus pyralis萤光素酶的氨基酸残基310相对应的氨基酸残基; 与出现在相应野生型序列中的氨基酸不同，并且其中与在该位置具有相应野生型萤光素酶的氨基酸的酶相比，萤光素酶具有增加的热稳定性。

4. 发明公开

EP2071023A8 Novel enzyme 有权
标题翻译：新型酶
公开(公告)号：EP2071023A8
公开(公告)日：2010-06-02
申请号：EP08013254.1
申请日：1999-10-26
申请人： Promega Corporation
发明人： Squirrell, David James , Murphy, Melenie Jane , Leslie, Rachel Louise , Lowe, Christopher Robin , White, Peter John , Tisi, Laurence Carlo , Murray, James Augustus Henry
IPC分类号： C12N9/02 , A01H4/00
CPC分类号： C12N9/0069 , C12Q1/66
摘要： A protein having luciferase activity and at least 60% similarity to luciferase from Photinus pyralis, Luciola mingrelica, Luciola cruciata or Luciola lateralis, Hotaria paroula, Pyrophorus plagiophthalamus, Lampyris noctiluca, Pyrocoelia nayako or Photinus pennsylanvanica, wherein in the sequence of the enzyme, at least one of
(a) the amino acid residue corresponding to residue 214 in Photinus pyralis luciferase;
(b) the amino acid residue corresponding to residue 232 in Photinus pyralis luciferase;
(c) the amino acid residue corresponding to residue 295 in Photinus pyralis luciferase;
(d) the amino acid residue corresponding to amino acid 14 of the Photinus pyralis luciferase;
(e) the amino acid residue corresponding to amino acid 35 of the Photinus pyralis luciferase;
(f) the amino acid residue corresponding to amino acid residue 105 of the Photinus pyralis luciferase;
(g) the amino acid residue corresponding to amino acid residue 234 of the Photinus pyralis luciferase;
(h) the amino acid residue corresponding to amino acid residue 420 of the Photinus pyralis luciferase;
(i) the amino acid residue corresponding to amino acid residue 310 of the Photinus pyralis luciferase;
is different to the amino acid which appears in the corresponding wild type sequence and wherein the luciferase enzyme has increased thermostability as compared to an enzyme having the amino acid of the corresponding wild-type luciferase at this position.

5. 发明授权

EP2368977B1 Mutant luciferase having increased thermostability 有权
公开(公告)号：EP2368977B1
公开(公告)日：2018-06-27
申请号：EP10179370.1
申请日：1999-10-26
申请人： Promega Corporation
发明人： Squirrell, David James , Murphy, Melanie Jane , Leslie, Rachel Louise , Lowe, Christopher Robin , White, Peter John , Tisi, Laurence Carlo , Murray, James Augustus Henry
IPC分类号： C12N9/02 , A01H4/00
CPC分类号： C12N9/0069 , C12Q1/66
摘要： A protein having luciferase activity and at least 60% similarity to luciferase from Photinus pyralis, Luciola mingrelica, Luciola cruciata or Luciola lateralis, Hotaria paroula, Pyrophorus plagiophthalamus, Lampyris noctiluca, Pyrocoelia nayako or Photinus pennsylanvanica, wherein in the sequence of the enzyme, at least one of (a) the amino acid residue corresponding to residue 214 in Photinus pyralis luciferase; (b) the amino acid residue corresponding to residue 232 in Photinus pyralis luciferase; (c) the amino acid residue corresponding to residue 295 in Photinus pyralis luciferase; (d) the amino acid residue corresponding to amino acid 14 of the Photinus pyralis luciferase; (e) the amino acid residue corresponding to amino acid 35 of the Photinus pyralis luciferase; (f) the amino acid residue corresponding to amino acid residue 105 of the Photinus pyralis luciferase; (g) the amino acid residue corresponding to amino acid residue 234 of the Photinus pyralis luciferase; (h) the amino acid residue corresponding to amino acid residue 420 of the Photinus pyralis luciferase; (i) the amino acid residue corresponding to amino acid residue 310 of the Photinus pyralis luciferase; is different to the amino acid which appears in the corresponding wild type sequence and wherein the luciferase enzyme has increased thermostability as compared to an enzyme having the amino acid of the corresponding wild-type luciferase at this position.

6. 发明授权

EP2366778B1 Mutant luciferase having increased thermostability 有权
标题翻译：突变荧光素酶具有改善的热稳定性
公开(公告)号：EP2366778B1
公开(公告)日：2012-08-22
申请号：EP10179359.4
申请日：1999-10-26
申请人： Promega Corporation
发明人： Squirrell, David James , Murphy, Melanie Jane , Leslie, Rachel Louise , Lowe, Christopher Robin , White, Peter John , Tisi, Laurence Carlo , Murray, James Augustus Henry
IPC分类号： C12N9/02 , A01H4/00
CPC分类号： C12N9/0069 , C12Q1/66

7. 发明公开

EP2368976A1 Mutant luciferase having increased thermostability 审中-公开
公开(公告)号：EP2368976A1
公开(公告)日：2011-09-28
申请号：EP10179361.0
申请日：1999-10-26
申请人： Promega Corporation
发明人： Squirrell, David James , Murphy, Melanie Jane , Leslie, Rachel Louise , Lowe, Christopher Robin , White, Peter John , Tisi, Laurence Carlo , Murray, James Augustus Henry
IPC分类号： C12N9/02 , A01H4/00
CPC分类号： C12N9/0069 , C12Q1/66
摘要： A protein having luciferase activity and at least 60% similarity to luciferase from Photinus pyralis, Luciola mingrelica, Luciola cruciata or Luciola lateralis, Hotaria paroula, Pyrophorus plagiophthalamus, Lampyris noctiluca, Pyrocoelia nayako or Photinus pennsylanvanica, wherein in the sequence of the enzyme, at least one of
(a) the amino acid residue corresponding to residue 214 in Photinus pyralis luciferase;
(b) the amino acid residue corresponding to residue 232 in Photinus pyralis luciferase;
(c) the amino acid residue corresponding to residue 295 in Photinus pyralis luciferase;
(d) the amino acid residue corresponding to amino acid 14 of the Photinus pyralis luciferase;
(e) the amino acid residue corresponding to amino acid 35 of the Photinus pyralis luciferase;
(f) the amino acid residue corresponding to amino acid residue 105 of the Photinus pyralis luciferase;
(g) the amino acid residue corresponding to amino acid residue 234 of the Photinus pyralis luciferase;
(h) the amino acid residue corresponding to amino acid residue 420 of the Photinus pyralis luciferase;
(i) the amino acid residue corresponding to amino acid residue 310 of the Photinus pyralis luciferase;
is different to the amino acid which appears in the corresponding wild type sequence and wherein the luciferase enzyme has increased thermostability as compared to an enzyme having the amino acid of the corresponding wild-type luciferase at this position.

8. 发明公开

EP2366780A1 Mutant luciferase having increased thermostability 有权
标题翻译：突变型萤光素酶具有增加的热稳定性
公开(公告)号：EP2366780A1
公开(公告)日：2011-09-21
申请号：EP10179373.5
申请日：1999-10-26
申请人： Promega Corporation
发明人： Squirrell, David James , Murphy, Melanie Jane , Leslie, Rachel Louise , Lowe, Christopher Robin , White, Peter John , Tisi, Laurence Carlo , Murray, James Augustus Henry
IPC分类号： C12N9/02 , A01H4/00
CPC分类号： C12N9/0069 , C12Q1/66
摘要： A protein having luciferase activity and at least 60% similarity to luciferase from Photinus pyralis, Luciola mingrelica, Luciola cruciata or Luciola lateralis, Hotaria paroula, Pyrophorus plagiophthalamus, Lampyris noctiluca, Pyrocoelia nayako or Photinus pennsylanvanica, wherein in the sequence of the enzyme, at least one of
(a) the amino acid residue corresponding to residue 214 in Photinus pyralis luciferase;
(b) the amino acid residue corresponding to residue 232 in Photinus pyralis luciferase;
(c) the amino acid residue corresponding to residue 295 in Photinus pyralis luciferase;
(d) the amino acid residue corresponding to amino acid 14 of the Phatinus pyralis luciferase;
(e) the amino acid residue corresponding to amino acid 35 of the Photinus pyralis luciferase;
(f) the amino acid residue corresponding to amino acid residue 105 of the Photinus pyralis luciferase;
(g) the amino acid residue corresponding to amino acid residue 234 of the Photinus pyralis luciferase;
(h) the amino acid residue corresponding to amino acid residue 420 of the Photinus pyralis luciferase;
(i) the amino acid residue corresponding to amino acid residue 310 of the Photinus pyralis luciferase;
is different to the amino acid which appears in the corresponding wild type sequence and wherein the luciferase enzyme has increased thermostability as compared to an enzyme having the amino acid of the corresponding wild-type luciferase at this position.
摘要翻译：一种具有荧光素酶活性并与来自Photinus pyralis，Luciola mingrelica，Cruciata或Luciola lateralis，Hotaria paroula，Pyrophorus plagiophthalamus，Lampyris noctiluca，Pyrocoelia nayako或Photinus pennsylanvanica的萤光素酶具有至少60％相似性的蛋白质，其中在所述酶的序列中，（a）对应于Photinus pyralis萤光素酶中残基214的氨基酸残基中的至少一个; （b）对应于Photinus pyralis萤光素酶中残基232的氨基酸残基; （c）对应于Photinus pyralis萤光素酶中残基295的氨基酸残基; （d）对应于Phatinus pyralis萤光素酶的氨基酸14的氨基酸残基; （e）与Photinus pyralis萤光素酶的第35位氨基酸对应的氨基酸残基; （f）对应于萤火虫萤光素酶的氨基酸残基105的氨基酸残基; （g）与Photinus pyralis萤光素酶的氨基酸残基234相对应的氨基酸残基; （h）与Photinus pyralis萤光素酶的氨基酸残基420相对应的氨基酸残基; （i）与Photinus pyralis萤光素酶的氨基酸残基310相对应的氨基酸残基; 与出现在相应野生型序列中的氨基酸不同，并且其中与在该位置具有相应野生型萤光素酶的氨基酸的酶相比，萤光素酶具有增加的热稳定性。

9. 发明授权

EP2366777B1 Mutant luciferase having increased thermostability 有权
公开(公告)号：EP2366777B1
公开(公告)日：2018-07-25
申请号：EP10179358.6
申请日：1999-10-26
申请人： Promega Corporation
发明人： Squirrell, David James , Murphy, Melanie Jane , Leslie, Rachel Louise , Lowe, Christopher Robin , White, Peter John , Tisi, Laurence Carlo , Murray, James Augustus Henry
IPC分类号： C12N9/02 , A01H4/00
CPC分类号： C12N9/0069 , C12Q1/66
摘要： A protein having luciferase activity and at least 60% similarity to luciferase from Photinus pyralis, Luciola mingrelica, Luciola cruciata or Luciola lateralis, Hotaria paroula, Pyrophorus plagiophthalamus, Lampyris noctiluca, Pyrocoelia nayako or Photinus pennsylanvanica, wherein in the sequence of the enzyme, at least one of (a) the amino acid residue corresponding to residue 214 in Photinus pyralis luciferase; (b) the amino acid residue corresponding to residue 232 in Photinus pyralis luciferase; (c) the amino acid residue corresponding to residue 295 in Photinus pyralis luciferase; (d) the amino acid residue corresponding to amino acid 14 of the Photinus pyralis luciferase; (e) the amino acid residue corresponding to amino acid 35 of the Photinus pyralis luciferase; (f) the amino acid residue corresponding to amino acid residue 105 of the Photinus pyralis luciferase; (g) the amino acid residue corresponding to amino acid residue 234 of the Photinus pyralis luciferase; (h) the amino acid residue corresponding to amino acid residue 420 of the Photinus pyralis luciferase; (i) the amino acid residue corresponding to amino acid residue 310 of the Photinus pyralis luciferase; is different to the amino acid which appears in the corresponding wild type sequence and wherein the luciferase enzyme has increased thermostability as compared to an enzyme having the amino acid of the corresponding wild-type luciferase at this position.

10. 发明授权

EP2366779B1 Mutant luciferase having increased thermostability 有权
公开(公告)号：EP2366779B1
公开(公告)日：2018-07-11
申请号：EP10179372.7
申请日：1999-10-26
申请人： Promega Corporation
发明人： Squirrell, David James , Murphy, Melanie Jane , Leslie, Rachel Louise , Lowe, Christopher Robin , White, Peter John , Tisi, Laurence Carlo , Murray, James Augustus Henry
IPC分类号： C12N9/02 , A01H4/00
CPC分类号： C12N9/0069 , C12Q1/66
摘要： A protein having luciferase activity and at least 60% similarity to luciferase from Photinus pyralis, Luciola mingrelica, Luciola cruciata or Luciola lateralis, Hotaria paroula, Pyrophorus plagiophthalamus, Lampyris noctiluca, Pyrocoelia nayako or Photinus pennsylanvanica, wherein in the sequence of the enzyme, at least one of (a) the amino acid residue corresponding to residue 214 in Photinus pyralis luciferase; (b) the amino acid residue corresponding to residue 232 in Photinus pyralis luciferase; (c) the amino acid residue corresponding to residue 295 in Photinus pyralis luciferase; (d) the amino acid residue corresponding to amino acid 14 of the Photinus pyralis luciferase; (e) the amino acid residue corresponding to amino acid 35 of the Photinus pyralis luciferase; (f) the amino acid residue corresponding to amino acid residue 105 of the Photinus pyralis luciferase; (g) the amino acid residue corresponding to amino acid residue 234 of the Photinus pyralis luciferase; (h) the amino acid residue corresponding to amino acid residue 420 of the Photinus pyralis luciferase; (i) the amino acid residue corresponding to amino acid residue 310 of the Photinus pyralis luciferase; is different to the amino acid which appears in the corresponding wild type sequence and wherein the luciferase enzyme has increased thermostability as compared to an enzyme having the amino acid of the corresponding wild-type luciferase at this position.

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