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1. 发明公开

EP2281049A2 COMPOSITIONS AND METHODS FOR THE SUPPRESSION OF TARGET POLYNUCLEOTIDES FROM THE FAMILY APHIDIDAE 审中-公开
标题翻译：成分和方法靶多来自蚜科抑
公开(公告)号：EP2281049A2
公开(公告)日：2011-02-09
申请号：EP09701991.3
申请日：2009-01-15
申请人： Pioneer Hi-Bred International Inc. , E. I. du Pont de Nemours and Company
发明人： HERRMANN, Rafael , LASSNER, Michael , LU, Albert, L. , NELSON, Mark , PRESNAIL, James, K. , RICE, Janet, A.
IPC分类号： C12N15/82
CPC分类号： C12N15/8286 , C07K14/43563 , Y02A40/162
摘要： Methods and compositions are provided which employ a silencing element that, when ingested by a pest, such as a pest from the Aphididae family, they are capable of decreasing the expression of a target sequence in the pest. In specific embodiments, the decrease in expression of the target sequence controls the pest and thereby the methods and compositions are capable of limiting damage to a plant. The present invention provides target polynucleotides for specific protein classes and also target polynucleotides as set forth in SEQ ID NOS:1, 2, 3, 4, 5, 6, 7, 8, 9, 10 or active variants or fragments thereof, wherein a decrease in expression of one or more the sequences in the target pest controls the pest (i.e., has insecticidal activity). Further provided are silencing elements which when ingested by the pest decrease the level of the target polypeptide and thereby control the pest. In specific embodiment, the pest is Aphis glycines. Plants, plant part, bacteria and other host cells comprising the silencing elements or an active variant or fragment thereof of the invention are also provided.

2. 发明授权

EP1763536B1 ANTIFUNGAL POLYPEPTIDES 失效转让
标题翻译：抗真菌多肽
公开(公告)号：EP1763536B1
公开(公告)日：2010-09-08
申请号：EP05788923.0
申请日：2005-07-01
申请人： Pioneer-Hi-Bred International, Inc. , E. I. du Pont de Nemours and Company , The Regents of The University of California
发明人： ALTIER, Daniel, J. , DAHLBACKA, Glen , HERRMANN, Rafael , HUNTER-CEVERA, Jennie , McCUTCHEN, Billy, Fred , PRESNAIL, James, K. , SCHEPERS, Eric, J. , RICE, Janet, A. , SIMMONS, Carl, R. , TOROK, Tamas , YALPANI, Nasser , ELLESKAYA, I., A.
IPC分类号： C07K14/385 , C07K14/37
CPC分类号： C07K14/37 , A01N63/02 , A01N63/04 , A61K38/00 , C07K14/385 , C12N15/8282
摘要： Compositions and methods for protecting a plant from a pathogen, particularly a fungal pathogen, are provided. Compositions include novel amino acid sequences, and variants and fragments thereof, for antipathogenic polypeptides that were isolated from microbial fermentation broths. Nucleic acid molecules comprising nucleotide sequences that encode the antipathogenic polypeptides of the invention are also provided. A method for inducing pathogen resistance in a plant using the nucleotide sequences disclosed herein is further provided. The method comprises introducing into a plant an expression cassette comprising a promoter operably linked to a nucleotide sequence that encodes an antipathogenic polypeptide of the invention. Compositions comprising an antipathogenic polypeptide or a transformed microorganism comprising a nucleic acid of the invention in combination with a carrier and methods of using these compositions to protect a plant from a pathogen are further provided. Transformed plants, plant cells, seeds, and microorganisms comprising a nucleotide sequence that encodes an antipathogenic polypeptide of the invention, or variant or fragment thereof, are also disclosed.

3. 发明授权

EP2897453B1 MANAGEMENT OF CORN ROOTWORM AND OTHER INSECT PESTS 有权转让
标题翻译：玉米螟和其他昆虫害虫的管理
公开(公告)号：EP2897453B1
公开(公告)日：2018-03-28
申请号：EP13839524.9
申请日：2013-09-20
申请人： E. I. du Pont de Nemours and Company , Pioneer Hi-Bred International, Inc. , Purdue Research Foundation
发明人： FLEXNER, John, Lindsey , JOHAL, Gurmukh, S. , MULTANI, Dilbag, S. , PRESNAIL, James, K. , TINGEY, Scott, V.
IPC分类号： A01H5/00 , A01H1/00 , C12N15/82 , A01G9/08
CPC分类号： C12N15/8286 , A01B79/02 , A01H5/10 , C07K14/415
摘要： Trap and/or refuge crops and methods of use in managing corn rootworm and other insect pests of maize are provided. Some methods involve using trap crops comprising plants with enhanced susceptibility to one or more insect pests to lure and kill insect pests. Other methods involve using refuge crops comprising plants with enhanced susceptibility to one or more insect pests to monitor insect pest populations in an area or to promote mating between insecticide resistant and insecticide non-resistant insects.

4. 发明授权

EP1385370B1 ANIMICROBIAL POLYPEPTIDES AND THEIR USES 有权
标题翻译：抗微生物多肽及其用途
公开(公告)号：EP1385370B1
公开(公告)日：2008-02-20
申请号：EP02726780.6
申请日：2002-04-19
申请人： PIONEER HI-BRED INTERNATIONAL, INC. , E. I. DU PONT DE NEMOURS AND COMPANY
发明人： ALTIER, Daniel, J. , HERRMANN, Rafael , LU, Albert, L. , McCUTCHEN, Billy, F. , PRESNAIL, James, K. , WEAVER, Janine, L. , WONG, James, F., H.
IPC分类号： A01H5/00 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/04 , C12N7/01 , C12N15/12 , C12N15/70 , C12N15/74 , C12N15/80 , C12N15/81 , C12N15/82 , C07K14/435
CPC分类号： C12N15/8282 , C07K14/43563 , Y10S530/858
摘要： The methods and compositions of the present invention find use in impacting microbial pathogens and in enhancing disease resistance to pathogens, particularly by plants. The compositions of the invention include polypeptides that possess antimicrobial properties, particularly fungicidal properties, and the encoding nucleic acid molecules. The polypeptides of the invention are isolated from the hemolymph and fat bodies of insect larvae induced by injection of plant pathogenic fungi. Further provided are plant cells, plants, an seed thereof, transformed with the nucleic acid mole cules of the invention so as to confer disease resistance on the plant.

5. 发明公开

EP2897453A2 MANAGEMENT OF CORN ROOTWORM AND OTHER INSECT PESTS 有权转让
标题翻译： ANTI-玉米根系演习和其他害虫
公开(公告)号：EP2897453A2
公开(公告)日：2015-07-29
申请号：EP13839524.9
申请日：2013-09-20
申请人： E. I. du Pont de Nemours and Company , Pioneer Hi-Bred International, Inc. , Purdue Research Foundation
发明人： FLEXNER, John, Lindsey , JOHAL, Gurmukh, S. , MULTANI, Dilbag, S. , PRESNAIL, James, K. , TINGEY, Scott, V.
IPC分类号： A01H5/00 , A01H1/00 , C12N15/82 , A01G9/08
CPC分类号： C12N15/8286 , A01B79/02 , A01H5/10 , C07K14/415
摘要： Trap and/or refuge crops and methods of use in managing corn rootworm and other insect pests of maize are provided. Some methods involve using trap crops comprising plants with enhanced susceptibility to one or more insect pests to lure and kill insect pests. Other methods involve using refuge crops comprising plants with enhanced susceptibility to one or more insect pests to monitor insect pest populations in an area or to promote mating between insecticide resistant and insecticide non- resistant insects.

6. 发明公开

EP2470662A1 COMPOSITIONS AND METHODS TO CONTROL INSECT PESTS 有权转让
标题翻译：成分和方法病虫害防治
公开(公告)号：EP2470662A1
公开(公告)日：2012-07-04
申请号：EP10749568.1
申请日：2010-08-26
申请人： E. I. du Pont de Nemours and Company
发明人： BROGLIE, Karen, E. , KRISS, Kevin , LU, Albert, Laurence , MUTHALAGI, Mani , PRESNAIL, James, K.
IPC分类号： C12N15/82 , A01H5/00
CPC分类号： A01N57/16 , A01N63/00 , A01N65/20 , A01N65/44 , C12N15/113 , C12N15/8218 , C12N15/8286 , C12N2310/11 , C12N2310/531 , Y02A40/162
摘要： Methods and compositions are provided which employ a silencing element that, when ingested by a pest, such as a Coleopteran plant pest or a Diabrotica plant pest decrease the expression of a target sequence in the pest. In specific embodiments, the decrease in expression of the target sequence controls the pest and thereby the methods and compositions are capable of limiting damage to a plant. The present invention provides various target polynucleotides set forth in anyone of SEQ ID NOS: 1-236 or active variants and fragments thereof, wherein a decrease in expression of one or more the sequences in the target pest controls the pest (i.e., has insecticidal activity). Further provided are silencing elements which when ingested by the pest decrease the level of the target polypeptide and thereby control the pest. In specific embodiment, the pest is D. virgifera virgifera, D. barber i, D. speciosa, or D. undecimpimctata howardi, Plants, plant part, bacteria and other host cells comprising the silencing elements or an active variant or fragment thereof of the invention are also provided.

7. 发明授权

EP3098317B1 COMPOSITIONS AND METHODS TO CONTROL INSECT PESTS 有权转让
公开(公告)号：EP3098317B1
公开(公告)日：2018-08-22
申请号：EP16177805.5
申请日：2010-08-26
申请人： E. I. du Pont de Nemours and Company
发明人： BROGLIE, Karen, E. , KRISS, Kevin , LU, Albert, Laurence , MUTHALAGI, Mani , PRESNAIL, James, K.
IPC分类号： C12N15/82 , A01H5/00
CPC分类号： A01N57/16 , A01N63/00 , A01N65/20 , A01N65/44 , C12N15/113 , C12N15/8218 , C12N15/8286 , C12N2310/11 , C12N2310/531 , Y02A40/162
摘要： Methods and compositions are provided which employ a silencing element that, when ingested by a pest, such as a Coleopteran plant pest or a Diabrotica plant pest decrease the expression of a target sequence in the pest. In specific embodiments, the decrease in expression of the target sequence controls the pest and thereby the methods and compositions are capable of limiting damage to a plant. The present invention provides various target polynucleotides set forth in anyone of SEQ ID NOS: 1-236 or active variants and fragments thereof, wherein a decrease in expression of one or more the sequences in the target pest controls the pest (i.e., has insecticidal activity). Further provided are silencing elements which when ingested by the pest decrease the level of the target polypeptide and thereby control the pest. In specific embodiment, the pest is D. virgifera virgifera, D. barber i, D. speciosa, or D. undecimpimctata howardi, Plants, plant part, bacteria and other host cells comprising the silencing elements or an active variant or fragment thereof of the invention are also provided.

8. 发明公开

EP3098317A1 COMPOSITIONS AND METHODS TO CONTROL INSECT PESTS 有权转让
标题翻译：控制昆虫害虫的组合物和方法
公开(公告)号：EP3098317A1
公开(公告)日：2016-11-30
申请号：EP16177805.5
申请日：2010-08-26
申请人： E. I. du Pont de Nemours and Company
发明人： BROGLIE, Karen, E. , KRISS, Kevin , LU, Albert, Laurence , MUTHALAGI, Mani , PRESNAIL, James, K.
IPC分类号： C12N15/82 , A01H5/00
CPC分类号： A01N57/16 , A01N63/00 , A01N65/20 , A01N65/44 , C12N15/113 , C12N15/8218 , C12N15/8286 , C12N2310/11 , C12N2310/531 , Y02A40/162
摘要： Methods and compositions are provided which employ a silencing element that, when ingested by a pest, such as a Coleopteran plant pest or a Diabrotica plant pest decrease the expression of a target sequence in the pest. In specific embodiments, the decrease in expression of the target sequence controls the pest and thereby the methods and compositions are capable of limiting damage to a plant. The present invention provides various target polynucleotides set forth in anyone of SEQ ID NOS: 1-236 or active variants and fragments thereof, wherein a decrease in expression of one or more the sequences in the target pest controls the pest (i.e., has insecticidal activity). Further provided are silencing elements which when ingested by the pest decrease the level of the target polypeptide and thereby control the pest. In specific embodiment, the pest is D. virgifera virgifera, D. barber i, D. speciosa, or D. undecimpimctata howardi, Plants, plant part, bacteria and other host cells comprising the silencing elements or an active variant or fragment thereof of the invention are also provided.
摘要翻译：提供了使用沉默元件的方法和组合物，所述沉默元件当被有害生物例如鞘翅目植物害虫或Diabrotica植物害虫摄入时减少害虫中靶序列的表达。在具体的实施方案中，靶序列表达的降低控制害虫，并且由此该方法和组合物能够限制对植物的损害。本发明提供了在SEQ ID NO：1-236中任何一个所列的各种靶多核苷酸或其活性变体和片段，其中目标害虫中一个或多个序列的表达降低控制害虫（即具有杀虫活性）。进一步提供了沉默元件，其在被害虫摄入时降低靶多肽的水平并由此控制害虫。在具体的实施方案中，害虫是D.virgifera virgifera，D. barber i，D. speciosa或D. undecimpimctata howardi，植物，植物部分，细菌和其他宿主细胞，其包含所述沉默元件或其活性变体或片段还提供了发明。

9. 发明授权

EP2470662B1 COMPOSITIONS AND METHODS TO CONTROL INSECT PESTS 有权转让
标题翻译：成分和方法病虫害防治
公开(公告)号：EP2470662B1
公开(公告)日：2016-08-10
申请号：EP10749568.1
申请日：2010-08-26
申请人： E. I. du Pont de Nemours and Company
发明人： BROGLIE, Karen, E. , KRISS, Kevin , LU, Albert, Laurence , MUTHALAGI, Mani , PRESNAIL, James, K.
IPC分类号： C12N15/82 , A01H5/00
CPC分类号： A01N57/16 , A01N63/00 , A01N65/20 , A01N65/44 , C12N15/113 , C12N15/8218 , C12N15/8286 , C12N2310/11 , C12N2310/531 , Y02A40/162
摘要： Methods and compositions are provided which employ a silencing element that, when ingested by a pest, such as a Coleopteran plant pest or a Diabrotica plant pest decrease the expression of a target sequence in the pest. In specific embodiments, the decrease in expression of the target sequence controls the pest and thereby the methods and compositions are capable of limiting damage to a plant. The present invention provides various target polynucleotides set forth in anyone of SEQ ID NOS: 1-236 or active variants and fragments thereof, wherein a decrease in expression of one or more the sequences in the target pest controls the pest (i.e., has insecticidal activity). Further provided are silencing elements which when ingested by the pest decrease the level of the target polypeptide and thereby control the pest. In specific embodiment, the pest is D. virgifera virgifera, D. barber i, D. speciosa, or D. undecimpimctata howardi, Plants, plant part, bacteria and other host cells comprising the silencing elements or an active variant or fragment thereof of the invention are also provided.

10. 发明授权

EP2027276B1 ANTIFUNGAL POLYPEPTIDES 有权
标题翻译：抗真菌多肽
公开(公告)号：EP2027276B1
公开(公告)日：2011-02-02
申请号：EP07845231.5
申请日：2007-05-15
申请人： Pioneer Hi-Bred International, Inc. , E.I. DU PONT DE NEMOURS AND COMPANY , The Regents of the University of California
发明人： ALTIER, Daniel, J. , CRANE, Virginia, C. , GILLIAM, Jacob, T. , HUNTER-CEVERA, Jennie , PRESNAIL, James, K. , SCHEPERS, Eric, J. , SIMMONS, Carl, R. , TOROK, Tamas , YALPANI, Nasser , ELLANSKAYA, I.A.
IPC分类号： C12N15/82 , C07K14/385
CPC分类号： C12N15/8282
摘要： Compositions and methods for protecting a plant from a pathogen, particularly a fungal pathogen, are provided. Compositions include amino acid sequences, and variants and fragments thereof, for antipathogenic polypeptides that were isolated from fungal fermentation broths. Nucleic acids that encode the antipathogenic polypeptides are also provided. A method for inducing pathogen resistance in a plant using the nucleotide sequences disclosed herein is further provided. The method comprises introducing into a plant an expression cassette comprising a promoter operably linked to a nucleotide sequence that encodes an antipathogenic polypeptide of the invention. Compositions comprising an antipathogenic polypeptide or a transformed microorganism comprising a nucleic acid of the invention in combination with a carrier and methods of using these compositions to protect a plant from a pathogen are further provided. Transformed plants, plant cells, seeds, and microorganisms comprising a nucleotide sequence that encodes an antipathogenic polypeptide of the invention are also disclosed.

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