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1. 发明公开

EP2336153A1 Improved expression systems with SEC-system secretion 有权转让
标题翻译：具有SEC系统分泌的改进的表达系统
公开(公告)号：EP2336153A1
公开(公告)日：2011-06-22
申请号：EP10179651.4
申请日：2004-11-22
申请人： Pfenex, Inc.
发明人： Retallack, Diane M. , Schneider, Jane C. , Ramseier, Thomas Martin
IPC分类号： C07K14/21 , C07K14/61 , C07K16/16 , C12N15/62 , C12N15/78
CPC分类号： C07K14/21 , C07K14/61 , C07K16/00 , C07K16/16 , C07K2317/622 , C07K2319/036 , C12N15/625 , C12N15/78
摘要： Improved methods and prokaryotic expression systems for producing recombinant proteins using Sec-system secretion signal peptides are described using Pseudomonas fluorescens Sec secretion systems. Specific novel Sec-system secretion signal peptides are described, as are fusion proteins and coding sequences for improved secretion of recombinant proteins and peptides.
摘要翻译：用荧光假单胞菌Sec分泌系统描述了使用Sec系统分泌信号肽生产重组蛋白的改进方法和原核表达系统。描述了特定的新型Sec系统分泌信号肽，融合蛋白和用于改善重组蛋白和肽分泌的编码序列。

2. 发明公开

EP2615172A1 Method for rapidly screening microbial hosts to identify certain strains with improved yield and/or quality in the expression of heterologous proteins 审中-公开
标题翻译：快速筛选方法的细菌宿主用于识别的异源蛋白质的表达具有改善的产率和/或改进的质量特殊菌株
公开(公告)号：EP2615172A1
公开(公告)日：2013-07-17
申请号：EP12198545.1
申请日：2008-04-25
申请人： Pfenex Inc.
发明人： Ramseier, Thomas M. , Coleman, Russell John , Schneider, Jane C. , Hershberger, Charles Douglas
IPC分类号： C12N15/78 , C12Q1/68
CPC分类号： C12Q1/689 , C12N1/20 , C12N15/78
摘要： The present invention provides an array for rapidly identifying a host cell population capable of producing heterologous protein with improved yield and/or quality. The array comprises one or more host cell populations that have been genetically modified to increase the expression of one or more target genes involved in protein production, decrease the expression of one or more target genes involved in protein degradation, or both. One or more of the strains in the array may express the heterologous protein of interest in a periplasm compartment, or may secrete the heterologous protein extracellularly through an outer cell wall. The strain arrays are useful for screening for improved expression of any protein of interest, including therapeutic proteins, hormones, a growth factors, extracellular receptors or ligands, proteases, kinases, blood proteins, chemokines, cytokines, antibodies and the like.
摘要翻译：本发明提供了一种阵列，用于快速识别一个能够产生具有提高的产量和/或质量的异源蛋白质的宿主细胞群。所述阵列包括一个或多个宿主细胞群体thathave被遗传修饰，以增加的蛋白生产涉及一种或多种靶基因的表达，减少的蛋白质降解，或二者参与一种或多种靶基因的表达。的一个或多个阵列中的菌株可以表达的异源兴趣蛋白质在周质隔室，或者可通过对细胞外壁在细胞外分泌的异源蛋白质。应变阵列是用于筛选感兴趣的任何蛋白质的提高的表达，包括治疗性蛋白，激素，生长因子，细胞外受体或配体，蛋白酶，激酶，血蛋白，趋化因子，细胞因子，抗体等是有用的。

3. 发明授权

EP2336153B1 Improved expression systems with SEC-system secretion 有权转让
标题翻译：改善表达系统与SEC系统分泌
公开(公告)号：EP2336153B1
公开(公告)日：2016-03-30
申请号：EP10179651.4
申请日：2004-11-22
申请人： Pfenex Inc.
发明人： Retallack, Diane M. , Schneider, Jane C. , Ramseier, Thomas Martin
IPC分类号： C07K14/21 , C07K14/61 , C07K16/16 , C12N15/62 , C12N15/78
CPC分类号： C07K14/21 , C07K14/61 , C07K16/00 , C07K16/16 , C07K2317/622 , C07K2319/036 , C12N15/625 , C12N15/78

4. 发明公开

EP2468869A1 Bacterial leader sequences for increased expression 有权转让
标题翻译： Bakterielle Leader-Sequenzenfürerhöhte表达
公开(公告)号：EP2468869A1
公开(公告)日：2012-06-27
申请号：EP11194072.2
申请日：2008-01-30
申请人： Pfenex, Inc.
发明人： Coleman, Russell John , Retallack, Diane M , Schneider, Jane C. , Ramseier, Thomas M. , Hershberger, Charles Douglas , Lee, Stacey, L , Resnick, Sol
IPC分类号： C12N15/62
CPC分类号： C12N15/625 , C07K2319/02 , C07K2319/036
摘要： Compositions and methods for improving expression and/or secretion of protein or polypeptide of interest in a host cell are provided. Compositions comprising a coding sequence for a bacterial secretion signal peptide are provided. The coding sequences can be used in vector constructs or expression systems for transformation and expression of a protein or polypeptide of interest in a host cell. The compositions of the invention are useful for increasing accumulation of properly processed proteins in the periplasmic space of a host cell, or for increasing secretion of properly processed proteins from the host cell. In particular, isolated secretion signal peptide-encoding nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the nucleic acid molecules are encompassed. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO: 18, and the nucleotide sequence set forth in SEQ ID NO: 17, as well as variants and fragments thereof, corresponding to Pseudomonas fluorescens FlgI secretion signal.
摘要翻译：提供用于改善宿主细胞中感兴趣的蛋白质或多肽的表达和/或分泌的组合物和方法。提供了包含细菌分泌信号肽的编码序列的组合物。编码序列可用于载体构建体或表达系统，用于在宿主细胞中转化和表达感兴趣的蛋白质或多肽。本发明的组合物可用于增加适当加工的蛋白质在宿主细胞的周质空间中的积聚，或用于增加来自宿主细胞的适当加工的蛋白质的分泌。特别地，提供了分离信号肽编码核酸分子。另外，包含对应于核酸分子的氨基酸序列。特别地，本发明提供了分离的核酸分子，其包含编码SEQ ID NO：18所示氨基酸序列的核苷酸序列和SEQ ID NO：17所示的核苷酸序列，以及其变体和片段，对应于到荧光假单胞菌FlgI分泌信号。

5. 发明授权

EP2327718B1 Improved expression systems with SEC-system secretion 有权转让
标题翻译：具有SEC系统分泌的改进的表达系统
公开(公告)号：EP2327718B1
公开(公告)日：2016-03-23
申请号：EP10179615.9
申请日：2004-11-22
申请人： Pfenex, Inc.
发明人： Retallack, Diane M. , Schneider, Jane C. , Ramseier, Thomas Martin
IPC分类号： C07K14/21 , C07K14/61 , C07K16/16 , C12N15/62 , C12N15/78
CPC分类号： C07K14/21 , C07K14/61 , C07K16/00 , C07K16/16 , C07K2317/622 , C07K2319/036 , C12N15/625 , C12N15/78

6. 发明公开

EP2327718A1 Improved expression systems with SEC-system secretion 有权转让
标题翻译：词汇表达系统使SEC系统分泌
公开(公告)号：EP2327718A1
公开(公告)日：2011-06-01
申请号：EP10179615.9
申请日：2004-11-22
申请人： Pfenex, Inc.
发明人： Retallack, Diane M. , Schneider, Jane C. , Ramseier, Thomas Martin
IPC分类号： C07K14/21 , C07K14/61 , C07K16/16 , C12N15/62 , C12N15/78
CPC分类号： C07K14/21 , C07K14/61 , C07K16/00 , C07K16/16 , C07K2317/622 , C07K2319/036 , C12N15/625 , C12N15/78
摘要： Improved methods and prokaryotic expression systems for producing recombinant proteins using Sec-system secretion signal peptides are described using Pseudomonas fluorescens Sec secretion systems. Specific novel Sec-system secretion signal peptides are described, as are fusion proteins and coding sequences for improved secretion of recombinant proteins and peptides.
摘要翻译：使用荧光假单胞菌分泌系统描述了使用Sec系统分泌信号肽生产重组蛋白质的改进方法和原核表达系统。描述了特异性新型Sec系统分泌信号肽，融合蛋白和用于改善重组蛋白和肽分泌的编码序列也是如此。

7. 发明公开

EP2314602A1 Improved expression systems with SEC-system secretion 审中-公开
公开(公告)号：EP2314602A1
公开(公告)日：2011-04-27
申请号：EP10179657.1
申请日：2004-11-22
申请人： Pfenex, Inc.
发明人： Retallack, Diane M. , Schneider, Jane C. , Ramseier, Thomas Martin
IPC分类号： C07K14/21 , C07K14/61 , C07K16/16 , C12N15/62 , C12N15/78
CPC分类号： C07K14/21 , C07K14/61 , C07K16/00 , C07K16/16 , C07K2317/622 , C07K2319/036 , C12N15/625 , C12N15/78
摘要： Improved methods and prokaryotic expression systems for producing recombinant proteins using Sec-system secretion signal peptides are described using Pseudomonas fluorescens Sec secretion systems. Specific novel Sec-system secretion signal peptides are described, as are fusion proteins and coding sequences for improved secretion of recombinant proteins and peptides.

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