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    • 4. 发明公开
    • FUMONISIN DETOXIFICATION COMPOSITIONS AND METHODS
    • Fumonisin脱毒组合物和方法
    • EP0988383A1
    • 2000-03-29
    • EP98933188.9
    • 1998-07-07
    • PIONEER HI-BRED INTERNATIONAL, INC.
    • DUVICK, JonathanMADDOX, Joyce, R.ROOD, Tracy, A.WANG, XunBOWEN, Benjamin, A.GILLIAM, Jacob, T.
    • C12N15/55C12N15/82C12N15/65C12Q1/44C12Q1/68
    • A01N63/00C12N9/00C12N9/16C12N9/18C12N15/8209C12N15/8242C12N15/8282
    • Methods for identifying organisms capable of degrading fumonisin. Fumonisin can be incorporated into culture medium for selection of organisms resistant to fumonisin and/or capable of growing on fumonisin as a sole carbon source. Using this method, several organisms have been identified. These organisms can be used to isolate the enzymes and the genes responsible for conferring fumonisin-resistance. The gene can be cloned and inserted into a suitable expression vector so that the protein can be further characterized. Additionally, the DNA encoding for fumonisin degrading enzymes can be used to transform plant cells normally susceptible to Fusarium or other toxin-producing fungus infections. Plants can be regenerated from the transformed plant cells. In this way, a transgenic plant can be produced with the capability of degrading fumonisin, as well as with the capability of producing the degrading enzymes. Methods for detoxification in grain, grain processing, silage, food crops and in animal feed and rumen microbes are also disclosed.
    • 用于鉴定能够降解伏马菌素的生物体的方法。 可以将伏马菌素掺入到培养基中以选择对伏马菌素有抗性和/或能够作为唯一碳源在伏马菌素上生长的生物体。 使用这种方法,已经确定了几种生物。 这些生物体可用于分离酶和负责赋予伏马菌素抗性的基因。 该基因可被克隆并插入合适的表达载体中,以便可进一步表征蛋白质。 此外,编码伏马菌素降解酶的DNA可用于转化通常对镰刀菌或其他产生毒素的真菌感染敏感的植物细胞。 植物可以从转化的植物细胞中再生。 通过这种方式,可以生产具有降解伏马菌素能力的转基因植物以及生产降解酶的能力。 还公开了谷物,谷物加工,青贮饲料,粮食作物以及动物饲料和瘤胃微生物的解毒方法。