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    • 3. 发明公开
    • METHOD FOR FLUORESCENTLY LABELING PROTEIN
    • VERFAHREN ZUR FLUORESZENZMARKIERUNG VON PROTEINEN
    • EP2399925A1
    • 2011-12-28
    • EP10750877.2
    • 2010-03-10
    • Osaka University
    • KIKUCHI, KazuyaHORI, YuichirouUENO, Hideki
    • C07K1/13C07D311/82C07D405/12C07D405/14C07K14/195C07K19/00C09K11/06C12N15/09
    • C07K14/215A61K49/0039A61K49/0043A61K49/0045A61K49/0056C07D311/82C07D405/12C07D405/14C07K1/13G01N33/533
    • A method for fluorescently labeling a protein is provided that can also be used for labeling a biomolecule (a protein) in vivo with fluorescence. The method for fluorescently labeling a protein includes obtaining a fusion protein of a target protein to be labeled and, for example, a photoactive yellow protein (PYP), and fluorescently labeling the target protein by reacting the fusion protein with a compound represented by Formula (I), wherein in the compound represented by Formula (I), a coumarin skeleton in Formula (A) or a benzene skeleton in Formula (B) is intramolecularly associated with a group X and as a result, fluorescence derived from the group X is suppressed and when in the compound of Formula (I), the coumarin skeleton in Formula (A) or the benzene skeleton in Formula (B) is bound to the PYP or the PYP-derived protein contained in the fusion protein, the coumarin skeleton in Formula (A) or the benzene skeleton in Formula (B) is intramolecularly dissociated from the group X, and thereby fluorescence derived from the group X can be emitted.
    • 提供了一种用于荧光标记蛋白质的方法,其也可用于在体内用荧光标记生物分子(蛋白质)。 荧光标记蛋白质的方法包括获得要标记的靶蛋白质和例如光活性黄蛋白(PYP)的融合蛋白,并通过使融合蛋白与由式(I)表示的化合物反应来荧光标记靶蛋白, I),其中在由式(I)表示的化合物中,式(A)中的香豆素骨架或式(B)中的苯骨架与基团X分子内缔合,结果,衍生自基团X的荧光是 抑制,当在式(I)的化合物中,式(A)中的香豆素骨架或式(B)中的苯骨架与包含在融合蛋白中的PYP或PYP衍生的蛋白质结合时,香豆素骨架在 式(B)中的式(A)或苯骨架从基团X分子内解离,从而可以发射衍生自基团X的荧光。