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2. 发明公开

EP3126522A1 DETERMINING ANTIGEN-SPECIFIC T-CELLS AND B-CELLS 无效
标题翻译： BESTIMMUNG VON ANTIGENSPEZIFISCHEN T-ZELLEN UND B-ZELLEN
公开(公告)号：EP3126522A1
公开(公告)日：2017-02-08
申请号：EP15772627.4
申请日：2015-04-01
申请人： Adaptive Biotechnologies Corp.
发明人： KLINGER, Mark , FAHAM, Malek
IPC分类号： C12Q1/68 , G01N33/00 , C12N5/07 , C12N5/16 , A61K39/395
摘要： The invention is directed to methods for determining antigen-specific T cells. In some embodiments, methods of the invention may be implemented by the steps of reacting under interaction conditions one or more antigens with T cells in a plurality of subsets of a tissue sample, such as peripheral blood; sorting antigen-interacting T cells from other T cells; separately sequencing for each subset recombined nucleic acid encoding a segment of a TCR chain from a sample of T cells prior to exposure to antigen and from a sample of T cells isolated based on their interaction with antigen, thereby forming a clonotype profile for the former sample and the latter sample for each subset; and identifying as antigen-specific T cells those T cells associated with a clonotype whose frequency increases in the latter sample relative to its frequency in the former sample.
摘要翻译：本发明涉及确定抗原特异性T细胞的方法。在一些实施方案中，本发明的方法可以通过以下步骤来实现：在相互作用条件下使一种或多种抗原与组织样品例如外周血中的多个子集中的T细胞反应; 从其他T细胞分选抗原相互作用的T细胞; 在暴露于抗原之前对来自T细胞样品的TCR链的片段和来自基于它们与抗原相互作用分离的T细胞样品的每个亚基重组核酸进行单独测序，由此形成前一样品的克隆型谱并且每个子集的后一个样本; 并且鉴定与后一样品中其频率在前一样品中频率增加的克隆型相关的那些T细胞作为抗原特异性T细胞。

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