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1. 发明公开

EP2031066A1 MUTANT AND GENE ENCODING THE SAME 审中-公开
标题翻译： DAFÜRKODIERENDES GEN
公开(公告)号：EP2031066A1
公开(公告)日：2009-03-04
申请号：EP07767052.9
申请日：2007-06-05
申请人： Nihon Shokuhin Kako Co., Ltd. , Japan Agency for Marine-Earth Science and Technology
发明人： NAKAGAWA, Yoshinori , TAKADA, Masayasu , OGAWA, Koichi , HATADA, Yuji , HORIKOSHI, Hiroki
IPC分类号： C12N15/54 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N9/10 , C12P21/02
CPC分类号： C12Y204/01019 , C12N9/1074 , C12P19/10
摘要： A mutant CGTase, having greater specific activity in the synthesis of gamma- cyclodextrin than the parent gamma -CGTase, in which at least one of the amino acid residues in the parent gamma -CGTase has been replaced with an amino acid residue differing from that particular amino acid residue in the parent gamma -CGTase. A gene encoding the amino acid sequence of the mutant. A method for manufacturing the mutant CGTase comprising: culturing the transformant that has been transformed or modified by chromosomal homologous recombination with the recombinant vector comprising the gene, and collecting the mutant CGTase produced by the transformant. Provided is an improved mutant CGTase that is imparted with greater specific activity than the parent gamma -CGTase by the addition of a mutation to gamma -CGTase, reducing the quantity of enzyme required to obtain a targeted CD composition. Provided is a gene encoding this mutant CGTase and a method for manufacturing the mutant CGTase employing this gene.
摘要翻译：一种突变体CGT酶，其在γ-环糊精的合成中具有比母体-GGTase更高的比活性，其中母体γ-GTase中的至少一个氨基酸残基被替换为与该特定的氨基酸残基不同的氨基酸残基母体γ-GTase中的氨基酸残基。编码突变体的氨基酸序列的基因。一种用于制备突变型CGT酶的方法，包括：通过与包含该基因的重组载体进行染色体同源重组而转化或修饰的转化体，并收集由转化体产生的突变型CGT酶。提供了一种改进的突变体CGTase，其通过向γ-GTTase添加突变赋予比母体γ-GTase更大的比活性，从而减少获得靶向CD组合物所需的酶的量。提供了编码该突变型CGT酶的基因和使用该基因的突变型CGTase的制造方法。

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