专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明公开

EP2251356A3 Polyclonal-monoclonal ELISA assay for detecting N-terminus proBNP 有权转让
标题翻译：多克隆单克隆ELISA分析N末端proBNP的所述标识
公开(公告)号：EP2251356A3
公开(公告)日：2011-08-31
申请号：EP10169301.8
申请日：2003-11-17
申请人： Nexus DX, Inc.
发明人： Davey, Michelle , Jackowski, George , Kupchak, Peter , Stanton, Eric
IPC分类号： C07K16/26 , G01N33/74
CPC分类号： C07K16/26 , C07K2317/34 , G01N33/6854 , G01N33/6887 , G01N33/74
摘要： A specific and sensitive in vitro ELISA assay and diagnostic test kit is disclosed for determining levels of NT-proBNP protein in a variety of bodily fluids, non-limiting examples of which are blood, serum, plasma, urine and the like. The NT-proBNP ELISA assay test employs the sandwich ELISA technique to measure circulating NT-proBNP in human plasma. In order to obtain antibodies with specific binding properties for targeted amino acid sequences within human proBNP, recombinant human proBNP (or rhproBNP) was expressed and purified for use as an immunogen. Polyclonal antibodies (PAb) to specific amino acid sequences were subsequently purified from goat serum by sequential affinity purification. Monoclonal antibodies were raised against specific polypeptides; Recombinant human NT-proBNP (or rhNT-proBNP) was expressed and purified in order to obtain material for use in calibration of a quantitative method for measurement of human NT-proBNP.

2. 发明授权

EP2251356B1 POLYCLONAL-MONOCLONAL ELISA ASSAY FOR DETECTING N-TERMINUS PROBNP 有权转让
标题翻译：多克隆单克隆ELISA测定用于N-端proBNP的的检测
公开(公告)号：EP2251356B1
公开(公告)日：2016-03-02
申请号：EP10169301.8
申请日：2003-11-17
申请人： Nexus DX, Inc.
发明人： Davey, Michelle , Jackowski, George , Kupchak, Peter , Stanton, Eric
IPC分类号： G01N33/68 , G01N33/74 , C07K16/26
CPC分类号： C07K16/26 , C07K2317/34 , G01N33/6854 , G01N33/6887 , G01N33/74

3. 发明公开

EP3061769A1 POLYCLONAL-MONOCLONAL ELISA ASSAY FOR DETECTING N-TERMINUS PROBNP 有权
标题翻译： POLYKLONALER-MONOKLONALER ENZYMGEKOPPELTER IMMUNADSORPTIONSTEST ZUR DETEKTION VON NT-PROBNP
公开(公告)号：EP3061769A1
公开(公告)日：2016-08-31
申请号：EP15203244.7
申请日：2003-11-17
申请人： Nexus DX, Inc.
发明人： Davey, Michelle , Jackowski, George , Kupchak, Peter , Stanton, Eric
IPC分类号： C07K16/26 , G01N33/74 , G01N33/68
CPC分类号： C07K16/26 , C07K2317/34 , G01N33/6854 , G01N33/6887 , G01N33/74
摘要： A specific and sensitive in vitro ELISA assay and diagnostic test kit is disclosed for determining levels of NT-proBNP protein in a variety of bodily fluids, non-limiting examples of which are blood, serum, plasma, urine and the like. The NT-proBNP ELISA assay test employs the sandwich ELISA technique to measure circulating NT-proBNP in human plasma. In order to obtain antibodies with specific binding properties for targeted amino acid sequences within human proBNP, recombinant human proBNP (or rhproBNP) was expressed and purified for use as an immunogen. Polyclonal antibodies (PAb) to specific amino acid sequences were subsequently purified from goat serum by sequential affinity purification. Monoclonal antibodies were raised against specific polypeptides; Recombinant human NT-proBNP (or rhNT-proBNP) was expressed and purified in order to obtain material for use in calibration of a quantitative method for measurement of human NT-proBNP.
摘要翻译：公开了一种特异性和敏感的体外ELISA测定和诊断测试试剂盒，用于测定各种体液中NT-proBNP蛋白的水平，其非限制性实例是血液，血清，血浆，尿液等。 NT-proBNP ELISA测定采用夹心ELISA技术测定人血浆中的NT-proBNP循环。为获得具有针对人proBNP内靶向氨基酸序列的特异性结合特性的抗体，重组人proBNP（或rhproBNP）被表达和纯化用作免疫原。随后通过连续亲和纯化从山羊血清中纯化特异性氨基酸序列的多克隆抗体（PAb）。针对特异性多肽产生单克隆抗体; 表达和纯化重组人NT-proBNP（或rhNT-proBNP），以获得用于校准人NT-proBNP的定量方法的材料。

4. 发明公开

EP3450457A1 POLYCLONAL-MONOCLONAL ELISA ASSAY FOR DETECTING N-TERMINUS PROBNP 审中-公开
公开(公告)号：EP3450457A1
公开(公告)日：2019-03-06
申请号：EP18186495.0
申请日：2003-11-17
申请人： Nexus DX, Inc.
发明人： Davey, Michelle , Jackowski, George , Kupchak, Peter , Stanton, Eric
IPC分类号： C07K16/26 , G01N33/74 , G01N33/68
摘要： A specific and sensitive in vitro ELISA assay and diagnostic test kit is disclosed for determining levels of NT-proBNP protein in a variety of bodily fluids, non-limiting examples of which are blood, serum, plasma, urine and the like. The NT-proBNP ELISA assay test employs the sandwich ELISA technique to measure circulating NT-proBNP in human plasma. In order to obtain antibodies with specific binding properties for targeted amino acid sequences within human proBNP, recombinant human proBNP (or rhproBNP) was expressed and purified for use as an immunogen. Polyclonal antibodies (PAb) to specific amino acid sequences were subsequently purified from goat serum by sequential affinity purification. Monoclonal antibodies were raised against specific polypeptides; Recombinant human NT-proBNP (or rhNT-proBNP) was expressed and purified in order to obtain material for use in calibration of a quantitative method for measurement of human NT-proBNP.

5. 发明授权

EP3061769B1 POLYCLONAL-MONOCLONAL ELISA ASSAY FOR DETECTING N-TERMINUS PROBNP 有权
公开(公告)号：EP3061769B1
公开(公告)日：2018-08-01
申请号：EP15203244.7
申请日：2003-11-17
申请人： Nexus DX, Inc.
发明人： Davey, Michelle , Jackowski, George , Kupchak, Peter , Stanton, Eric
IPC分类号： C07K16/26 , G01N33/74 , G01N33/68
CPC分类号： C07K16/26 , C07K2317/34 , G01N33/6854 , G01N33/6887 , G01N33/74
摘要： A specific and sensitive in vitro ELISA assay and diagnostic test kit is disclosed for determining levels of NT-proBNP protein in a variety of bodily fluids, non-limiting examples of which are blood, serum, plasma, urine and the like. The NT-proBNP ELISA assay test employs the sandwich ELISA technique to measure circulating NT-proBNP in human plasma. In order to obtain antibodies with specific binding properties for targeted amino acid sequences within human proBNP, recombinant human proBNP (or rhproBNP) was expressed and purified for use as an immunogen. Polyclonal antibodies (PAb) to specific amino acid sequences were subsequently purified from goat serum by sequential affinity purification. Monoclonal antibodies were raised against specific polypeptides; Recombinant human NT-proBNP (or rhNT-proBNP) was expressed and purified in order to obtain material for use in calibration of a quantitative method for measurement of human NT-proBNP.

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式