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    • 7. 发明公开
    • ALKALINE PROTEASE, PROCESS FOR THE PRODUCTION THEREOF, USE THEREOF, AND MICROORGANISM PRODUCING THE SAME
    • 碱性蛋白酶,一种产生,其应用方法,并且此生产微生物
    • EP0859050A4
    • 2001-10-17
    • EP96935529
    • 1996-11-01
    • NOVOZYMES AS
    • MIYOTA YOSHIAKIFUKUYAMA SHIROYONEDA TADASHI
    • C11D3/386C12N1/20C12N9/54C12P21/06C12R1/07C12N15/00
    • C12R1/07C11D3/386C11D3/38609C11D3/38618C12N9/54
    • A novel strain of Bacillus sp. (accession number: FERM BP-5736) and a protease which is obtained by incubating this strain or its variant, shows a high stability in the presence of surfactants, and has the following properties: (1) hydrolyzing proteins and peptides; (2) having an optimum pH value of around 12, when reacted with the use of casein as the substrate at 30 DEG C for 10 minutes; (3) having a stable pH value of from 5 to 11, when incubated with the use of casein as the substrate at 30 DEG C for 24 hours; (4) having an optimum action temperature of around 60 DEG C, when reacted with the use of casein as the substrate at pH 10 for 10 minutes; (5) having a molecular weight of 29,000 +/- 2,000 determined by SDS polyacrylamide gel electrophoresis; (6) having an isoelectric piont of 10.1 +/- 0.5 determined by isoelectric polyacrylamide gel electrphoresis; and (7) having a residual activity of 60 % or above in a 500 ppm LAS solution after 30 minutes at 40 DEG C or 20 % or above after 15 minutes at 55 DEG C.
    • 芽孢杆菌属的一个新菌株(保藏号FERM BP-5736)。,蛋白酶与由株或其突变体,其制造方法,以及使用的蛋白酶的培养获得以下性质的表面活性剂高度稳定。 (1)水解蛋白质或肽,在30℃下的用酪蛋白作为底物温育大约12(2)的最佳pH为24小时,(3)稳定的pH范围为5至11,用于温育以酪蛋白为底物 在30℃下24小时,(4)60℃左右的最适温度用酪蛋白作为底物温育的pH为10持续10分钟,通过SDS-聚丙烯酰胺凝胶的29000 +/- 2000(5)的分子量 电泳,(6)为10.1 +/- 0.5由等电的等电点聚焦聚丙烯酰胺凝胶电泳,和不小于60%,在40℃下在30分钟(7)的剩余活性用500ppm的LAS溶液混合后和 在55℃下不小于20%,15分钟后。