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1. 发明公开

EP0496345A1 Method for detecting and quantifying cariogenic bacteria 失效
标题翻译：巴伐利亚州的维拉法尼亚。
公开(公告)号：EP0496345A1
公开(公告)日：1992-07-29
申请号：EP92100923.9
申请日：1992-01-21
申请人： NAGASE & COMPANY, LTD.
发明人： Miyazaki, Toshitsugu , Matsuda, Yoko , Nakamura, Tsutomu , Ota, Fusao , Nishino, Mizuho
IPC分类号： G01N33/569 , C12Q1/04 , C12Q1/14 , C12Q1/06
CPC分类号： C12Q1/14 , G01N33/56944 , G01N2333/315
摘要： A novel method for detecting and quantifying cariogenic bacteria is provided, said method being characterized by the following steps:

(a) Streptococcus mutans in a sample to be examined is reacted with at least one polyclonal or monoclonal antibody having a specific reactivity to the microorganism;
(b) the antibody bound to the microorganism is separated from the unbound antibody by filtration through a membrane filter; and
(c) the bound antibody captured on the filter is detected by a suitable means.

The present method enables to rapidly and conveniently detect S. mutans in a high sensitivity, without the need of selective cultivation of a sample before detection, and without the problem of decrease of survival rate of bacteria caused by the time-lag between collection of a sample from a subject and its detection.
摘要翻译：提供了一种用于检测和定量致龋菌的新方法，所述方法的特征在于以下步骤：（a）待检样品中的变形链球菌与至少一种对微生物具有特异性反应性的多克隆或单克隆抗体反应; （b）通过膜过滤器过滤将与微生物结合的抗体与未结合的抗体分离; 和（c）通过合适的方法检测在过滤器上捕获的结合的抗体。本方法能够以高灵敏度快速方便地检测变形链球菌，而不需要在检测前对样品进行选择性培养，并且不存在由收集之前的时间滞后引起的细菌存活率降低的问题来自受试者的样本及其检测。

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