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3. 发明公开

EP2963416A1 A METHOD AND SYSTEM FOR MONITORING BIOMOLECULE SEPARATIONS BY MASS SPECTROMETRY 有权
标题翻译： VERFAHREN UND SYSTEM ZURÜBERWACHUNGDER TRENNUNGEN VONBIOMOLEKÜLENDURCH MASSENSPEKTROMETRIE
公开(公告)号：EP2963416A1
公开(公告)日：2016-01-06
申请号：EP15174507.2
申请日：2015-06-30
申请人： Microsaic Systems PLC
发明人： WRIGHT, Steven , McINTOSH, Alexander Iain
IPC分类号： G01N30/72 , G01N30/82 , G01N30/88 , G01N30/60
摘要： A means of monitoring biomolecule separations by mass spectrometry is described. The mixture to be separated is introduced into a first fluidic stream, which then passes through a chromatography column 103. Small samples are periodically taken from the first fluidic stream as it leaves the chromatography column and injected into a first branch of a second fluidic stream 212. Low molecular weight components detrimental to the efficient operation of the mass spectrometer are removed by an in-line dialysis cell 224. A second fraction of the second fluidic stream 214 acts as the dialysate. In a second and preferred system provided in accordance with the present teaching, the first fraction of the second fluidic stream is further split downstream of the sampling mechanism 215 through the use of a three-way connector 302. Approximately 0.3 to 5 microliters per minute continues through the dialysis cell and thereafter to the electrospray emitter 238 of the mass spectrometer. Desirably, the electrospray emitter 238, dialysis cell 224, and fluidic split 302 are provided as a demountable assembly 400. In a third embodiment of a system provided in accordance with the present teaching, the dialysate is provided separately as a third fluidic stream.
摘要翻译：描述了通过质谱监测生物分子分离的方法。要分离的混合物被引入第一流体流中，然后通过色谱柱103.当离开色谱柱并将其注入第二流体流212的第一分支时，从第一流体流中周期性地取出小样品通过在线透析池224去除对质谱仪的有效操作有害的低分子量组分。第二流体流214的第二部分用作透析液。在根据本教导提供的第二和优选系统中，第二流体流的第一部分通过使用三通连接器302进一步分离在采样机构215的下游。每分钟约0.3至5微升继续通过透析池，此后进入质谱仪的电喷发射器238。期望地，电喷射发射器238，透析池224和流体分流器302被提供为可拆卸组件400.在根据本教导提供的系统的第三实施例中，透析液单独提供为第三流体流。

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