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1. 发明公开

EP3111196A1 AIRBORNE MICROBIAL MEASUREMENT APPARATUS AND MEASUREMENT METHOD THEREOF 有权
标题翻译：拉斯维加斯国际米其林MESSVORRICHTUNG MESSVERFAHRENDAFÜR
公开(公告)号：EP3111196A1
公开(公告)日：2017-01-04
申请号：EP14883616.6
申请日：2014-09-19
申请人： LG Electronics Inc. , National University Corporation Toyohashi University of Technology
发明人： PARK, Chulwoo , LEE, Sunghwa , MIZUNO, Akira , TAKASHIMA, Kazunori , KURITA, Hirofumi , YASUDA, Hachiro
IPC分类号： G01N21/64 , G01N33/483 , C12Q1/68
CPC分类号： G01N15/0606 , C12Q1/04 , G01N15/0637 , G01N21/6428 , G01N21/76 , G01N21/8483 , G01N2015/0069 , G01N2021/6439
摘要： An airborne microbial measurement apparatus and a measurement method thereof are provided. An airborne microbial measurement apparatus according to an embodimentincludes a discharge apparatus including a discharge electrode and a voltage supply unit applying a high voltage to the discharge electrode. A substrate is provided to a side of the discharge apparatus to collect an airborne microbe from air by a high voltage applied to the discharge electrode. A reagent injection apparatus supplies a dyeing reagent to the microbe collected on the substrate or a DNA of the microbe. A light emission measurement apparatus senses a quantity of light generated from the DNA to which the dyeing reagent is supplied. The discharge apparatus includes a controller controlling the voltage supply unit so that the voltage is applied to collect the airborne microbe or destroy an external wall of the collected airborne microbe.
摘要翻译：提供了一种空气微生物测定装置及其测定方法。根据实施例的空气微生物测量装置包括放电装置，其包括放电电极和向放电电极施加高电压的电压供给单元。将基板设置在放电装置的一侧，以通过施加到放电电极的高电压从空气收集空气中的微生物。试剂注入装置将染色剂供给到收集在基底上的微生物或微生物的DNA。发光测定装置检测从供给染色剂的DNA所产生的光量。放电装置包括控制电压供应单元的控制器，以便施加电压以收集空气微生物或破坏收集的空气微生物的外壁。

2. 发明公开

EP2918667A1 FOREIGN SUBSTANCE INTRODUCTION DEVICE AND METHOD OF PRODUCING CELLS WITH INTRODUCED FOREIGN SUBSTANCE 有权
标题翻译：外来物质导入装置和方法细胞与综合外电物质的生产
公开(公告)号：EP2918667A1
公开(公告)日：2015-09-16
申请号：EP13861819.4
申请日：2013-12-12
申请人： National University Corporation Toyohashi University of Technology
发明人： MIZUNO, Akira , NUMANO, Rika , KURITA, Hirofumi
IPC分类号： C12M1/00 , C12M1/42 , C12N1/00 , C12N5/00 , C12N5/10
CPC分类号： C12M35/02 , C12N13/00 , C12N15/87
摘要： An exogenous substance transfer apparatus that introduces an exogenous substance into a small amount of cells efficiently and at low cost by electrical action and a method for manufacturing an exogenous substance-bearing cell that is able to manufacture many kinds of exogenous substance-bearing cells at low cost are provided.
In the introducing of an exogenous substance such as a gene or the like into a cell, a cell suspension liquid that contains the cell and the exogenous substance is supplied to a container (2) through an opening portion (17). The cell suspension liquid is supplied to the container (2) in an amount that is prepared such that a liquid droplet (W) that is formed will not come into contact with a pair of electrodes (5a, 5b) at the same time. The liquid droplet (W) is formed in an oil (6) that is stored in the container (2), without the cell suspension liquid mixing with the oil (6). The liquid droplet (W) is disposed between the electrodes (5a, 5b). When a direct current voltage is supplied to the electrodes (5a, 5b) from a power supply (9), the liquid droplet (W) moves between the electrodes (5a, 5b), and the exogenous substance is introduced into the cell within the liquid droplet (W) by electrical action when the liquid droplet (W) comes into contact with the electrodes (5a, 5b).
摘要翻译：外源物质传送装置那样推出到外源物质引入少量细胞的高效且通过电作用和用于制造外源物质轴承单元的方法低成本确实能够在低制造许多种外源物质载带单元的提供了成本。在将外源物质的导入：例如基因或类似物进入细胞，细胞悬浮液确实包含细胞和外源物质通过供给到容器（2），以开口部（17）。将细胞悬浮液的液体被供应到容器（2）量所做的是准备好的搜索做了液滴（W）做了形成不会接触到在在Sametime一对电极（5A，5B）。液滴（W）是在形成油（6）没有被存储在所述容器（2），而不会与油的细胞悬浮液混合（6）。液滴（W）在电极之间设置（5A，5B）。当直流电压被提供给电极（5A，5B）从电源（9），液滴（W）在电极之间移动时（图5a，5b），且外源性的物质被引入到内的细胞液滴（W）通过电作用当液滴（W）进入与电极接触（5A，5B）。

3. 发明授权

EP2918667B1 FOREIGN SUBSTANCE INTRODUCTION DEVICE AND METHOD OF PRODUCING CELLS WITH INTRODUCED FOREIGN SUBSTANCE 有权
标题翻译：外来物质引入装置和生产引入外来物质的细胞的方法
公开(公告)号：EP2918667B1
公开(公告)日：2017-11-01
申请号：EP13861819.4
申请日：2013-12-12
申请人： National University Corporation Toyohashi University of Technology
发明人： MIZUNO, Akira , NUMANO, Rika , KURITA, Hirofumi
IPC分类号： C12M1/00 , C12M1/42 , C12N1/00 , C12N5/00 , C12N5/10 , C12N15/87
CPC分类号： C12M35/02 , C12N13/00 , C12N15/87

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