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1. 发明公开

EP2481799A9 METHOD FOR TRANSFORMING STRAMENOPILE 审中-公开
标题翻译：转换STRAMENOPILE的方法
公开(公告)号：EP2481799A9
公开(公告)日：2013-02-13
申请号：EP10818879.8
申请日：2010-09-24
申请人： Kyushu University, National University Corporation , University of Miyazaki , Konan Gakuen , Nippon Suisan Kaisha, Ltd.
发明人： SAKAGUCHI, Keishi , MATSUDA, Takanori , KOBAYASHI, Takumi , ITO, Makoto , NAGANO, Naoki , HAYASHI, Masahiro , HONDA, Daisuke , TAOKA, Yosuke , OKITA, Yuji , IZUMIDA, Hitoshi , SUGIMOTO, Shinichi
IPC分类号： C12N15/09 , C11C3/00 , C12N1/15 , C12N9/02 , C12P7/64 , C12R1/645
CPC分类号： C12P7/6409 , C11C3/00 , C12N9/0071 , C12N9/0083 , C12N15/79 , C12N15/8201 , C12N15/8247 , C12P7/6427
摘要： Disclosed is a transformation method whereby an ability to produce a useful substance of a stramenopile can be improved. The method for transforming a stramenopile comprises transferring a foreign gene into the stramenopile which is a microorganism belonging to the class Labyrinthula, more specifically, to a genus Labyrinthula, Altornia, Aplanochytrium, Schizochytrium, Aurantiochytrium, Thraustochytrium, Ulkenia, etc. Said foreign gene, which is a gene relating to tolerance against an antibiotic, a colorimetric protein and/or a fatty acid desaturase (Δ5 desaturase gene, Δ12 desaturase gene and/or ω3 desaturase gene), is transferred by using the electroporation or gene-gun technique.
摘要翻译：公开了一种转化方法，由此可以提高生产菌株的有用物质的能力。用于转化原生藻菌的方法包括将外来基因转移到作为迷迭香属的微生物，更具体地讲是迷迭香属，Altornia属，Aplanochytrium属，Schizochytrium属，Aurantiochytrium属，破囊壶菌属，Ulkenia属等的原生藻属。所述外源基因，通过使用电穿孔或基因枪技术转移与抗生素，比色蛋白质和/或脂肪酸去饱和酶（Δ5去饱和酶基因，Δ12去饱和酶基因和/或ω3去饱和酶基因）耐受性有关的基因。

2. 发明公开

EP2623588A1 METHOD FOR TRANSFORMATION OF STRAMENOPILE 有权
标题翻译： VERFAHREN ZUR UMWANDLUNG VON STRAMENOPIL
公开(公告)号：EP2623588A1
公开(公告)日：2013-08-07
申请号：EP11829379.4
申请日：2011-09-30
申请人： Kyushu University National University Corporation , National University Corporation University Of Miyazaki , Konan Gakuen , Nippon Suisan Kaisha, Ltd.
发明人： SAKAGUCHI, Keishi , HAMAGUCHI, Rie , MATSUDA, Takanori , ITO, Makoto , NAGANO, Naoki , HAYASHI, Masahiro , HONDA, Daisuke , OKITA, Yuji , SUGIMOTO, Shinichi
IPC分类号： C12N1/15 , C12P7/64 , C12N9/02 , C12N9/10 , C12N15/09
CPC分类号： C12N15/79 , C12N9/0071 , C12N9/0083 , C12N9/1029 , C12N15/113 , C12N15/52 , C12N15/895 , C12N2310/11 , C12N2310/14 , C12P7/6409 , C12P7/6427 , C12P7/6472 , C12Y114/19001 , C12Y114/19006 , C12Y203/01119
摘要： [Problem] To provide a transformation method for producing a stramenopile organism having an improved unsaturated fatty acid production capability by disrupting a gene of the
stramenopile organism or inhibiting the expression of the gene in a genetically engineering manner. [Solution] A method for transforming a stramenopile organism, which comprises disrupting a gene of the stramenopile organism or inhibiting the expression of the gene in a genetically engineering manner, and which is characterized in that the stramenopile organism is selected from Thraustochytrium aureum, Parietichytrium sarkarianum, Thraustochytrium roseum and Parietichytrium sp. and the gene to be disrupted or of which the expression is to be inhibited is a gene associated with the biosynthesis of a fatty acid.
摘要翻译： [问题]提供一种通过破坏前体生物体的基因或以遗传工程方式抑制该基因的表达来生产具有改善的不饱和脂肪酸生产能力的延胡索生物的转化方法。 [解决方案]一种转基因生物体的方法，其包括以基因工程方式破坏所述前列腺生物体的基因或抑制所述基因的表达，其特征在于，所述前列腺生物体选自甲状腺杆菌（Thraustochytrium aureum），棒状杆菌（Parietichytrium sarkarianum），玫瑰色胸um和Parietichytrium sp。并且待破坏的基因或其表达被抑制的基因是与脂肪酸的生物合成相关的基因。

3. 发明公开

EP2966169A1 METHOD FOR TRANSFORMING STRAMENOPILES 审中-公开
标题翻译： VERFAHREN ZUR UMWANDLUNG VON STRAMENOPILEN
公开(公告)号：EP2966169A1
公开(公告)日：2016-01-13
申请号：EP15178199.4
申请日：2010-09-24
申请人： Kyushu University , University of Miyazaki , Konan Gakuen , NIPPON SUISAN KAISHA, LTD.
发明人： SAKAGUCHI, Keishi , MATSUDA, Takanori , KOBAYASHI, Takumi , ITO, Makoto , NAGANO, Naoki , HAYASHI, Masahiro , HONDA, Daisuke , TAOKA, Yosuke , OKITA, Yuji , IZUMIDA, Hitoshi , SUGIMOTO, Shinichi
IPC分类号： C12N15/09 , C11C3/00 , C12N1/15 , C12N9/02 , C12P7/64 , C12R1/645
CPC分类号： C12P7/6409 , C11C3/00 , C12N9/0071 , C12N9/0083 , C12N15/79 , C12N15/8201 , C12N15/8247 , C12P7/6427
摘要： Disclosed is a transformation method whereby an ability to produce a useful substance of a stramenopile can be improved. The method for transforming a stramenopile comprises transferring a foreign gene into the stramenopile which is a microorganism belonging to the class Labyrinthula, more specifically, to a genus Labyrinthula , Altornia , Aplanochytrium, Schizochytrium, Aurantiochytrium, Thraustochytrium, Ulkenia, etc. Said foreign gene, which is a gene relating to tolerance against an antibiotic, a colorimetric protein and/or a fatty acid desaturase (Δ5 desaturase gene, Δ12 desaturase gene and/or ω3 desaturase gene), is transferred by using the electroporation or gene-gun technique.
摘要翻译：公开了可以提高生产有机物的能力的转化方法。用于转化stramenopile的方法包括将外源基因转移到作为属于Labyrinthula类的微生物的更青霉属，更具体地是属于Labyrinthula，Altornia，Aplanochytrium，Schizochytrium，Aurantiochytrium，Thraustochytrium，Ulkenia等的所述外源基因，其是通过使用电穿孔或基因枪技术转移与抗生素，比色蛋白和/或脂肪酸去饱和酶（“5去饱和酶基因”，12去饱和酶基因和/或É3去饱和酶基因）有关的基因。

4. 发明公开

EP2481799A1 METHOD FOR TRANSFORMING STRAMENOPILE 审中-公开
标题翻译： VERFAHREN ZUR TRANSFORMATION VON STRAMENOPILEN
公开(公告)号：EP2481799A1
公开(公告)日：2012-08-01
申请号：EP10818879.8
申请日：2010-09-24
申请人： Kyushu University, National University Corporation , University of Miyazaki , Konan Gakuen , Nippon Suisan Kaisha, Ltd.
发明人： SAKAGUCHI, Keishi , MATSUDA, Takanobu , KOBAYASHI, Takumi , ITO, Makoto , NAGANO, Naoki , HAYASHI, Masahiro , HONDA, Daisuke , TAOKA, Yosuke , OKITA, Yuji , IZUMIDA, Hitoshi , SUGIMOTO, Shinichi
IPC分类号： C12N15/09 , C11C3/00 , C12N1/15 , C12N9/02 , C12P7/64 , C12R1/645
CPC分类号： C12P7/6409 , C11C3/00 , C12N9/0071 , C12N9/0083 , C12N15/79 , C12N15/8201 , C12N15/8247 , C12P7/6427
摘要： Disclosed is a transformation method whereby an ability to produce a useful substance of a stramenopile can be improved. The method for transforming a stramenopile comprises transferring a foreign gene into the stramenopile which is a microorganism belonging to the class Labyrinthula, more specifically, to a genus Labyrinthula, Altornia, Aplanochytrium, Schizochytrium, Aurantiochytrium, Thraustochytrium, Ulkenia, etc. Said foreign gene, which is a gene relating to tolerance against an antibiotic, a colorimetric protein and/or a fatty acid desaturase (Δ5 desaturase gene, Δ12 desaturase gene and/or ω3 desaturase gene), is transferred by using the electroporation or gene-gun technique.
摘要翻译：公开了可以提高生产有机物的能力的转化方法。用于转化stramenopile的方法包括将外源基因转移到作为属于Labyrinthula类的微生物的更青霉属，更具体地是属于Labyrinthula，Altornia，Aplanochytrium，Schizochytrium，Aurantiochytrium，Thraustochytrium，Ulkenia等的所述外源基因，其是通过使用电穿孔或基因枪技术转移与抗生素，比色蛋白和/或脂肪酸去饱和酶（“5去饱和酶基因”，12去饱和酶基因和/或É3去饱和酶基因）有关的基因。

5. 发明公开

EP2405018A1 METHOD FOR DETERMINING FATTY ACID SYNTHESIS PATHWAY OF MICROORGANISM, AND PCR PRIMER SET FOR USE IN THE METHOD 审中-公开
标题翻译：程序对测定微生物和PCR引物的脂肪酸合成PATH用于该方法
公开(公告)号：EP2405018A1
公开(公告)日：2012-01-11
申请号：EP10748811.6
申请日：2010-03-04
申请人： University of Miyazaki , Nippon Suisan Kaisha, Ltd. , Kyushu University, National University Corporation
发明人： NAGANO, Naoki , HAYASHI, Masahiro , SAKAGUCHI, Keishi , ITO, Makoto , TAOKA, Yosuke , OKITA, Yuji
IPC分类号： C12Q1/68 , C12N15/09
CPC分类号： C12Q1/689
摘要： Disclosed is a method for determining the fatty acid synthesis pathway of a microorganism. Specifically disclosed is a method for determining the fatty acid synthesis pathway of a microorganism, which is characterized by producing degenerate primers for a fatty acid synthesis-related enzyme gene and determining the presence or absence of the fatty acid synthesis-related gene in the genome gene extracted from the microorganism using the degenerate primers. The sequences for degenerate primers for C20-elongase gene, which is a fatty acid synthesis-related enzyme gene, are ATHGARTWYTKBRTITTYGTICA (SEQ ID NO:1) and TARTRISWRTACATIADIAMRTG (SEQ ID NO:2), and the sequences for degenerate primers for Δ4-desaturase gene are GGNCAYCAYCMITAYACNAA (SEQ ID NO:3) and TCDATYTGRTGIBWIARNCC (SEQ ID NO:4). The microorganism is one belonging to the class Labyrinthulea. Also specifically disclosed is a PCR primer set for use in the determination method. The method is useful as a method for screening a microorganism capable of synthesizing a fatty acid.
摘要翻译：公开了用于确定性的采矿方法的微生物的脂肪酸合成途径。具体地说游离缺失盘是由制造简并引物用于脂肪酸合成有关的酶基因和确定性采矿在基因组中基因的存在或不存在的脂肪酸合成相关的基因的表征确定性采矿的方法的微生物的脂肪酸合成途径，所有使用简并引物微生物提取。对于简并引物用于C20的序列延伸酶的基因，所有这些是脂肪酸合成相关酶的基因，是ATHGARTWYTKBRTITTYGTICA（SEQ ID NO：1）和TARTRISWRTACATIADIAMRTG（SEQ ID NO：2），以及用于简并引物的序列为“4 去饱和酶基因是GGNCAYCAYCMITAYACNAA（SEQ ID NO：3）和TCDATYTGRTGIBWIARNCC（SEQ ID NO：4）。微生物是一个属于类Labyrinthulea。所以特别游离缺失盘是用于在确定方法中使用的PCR引物组。该方法是作为用于筛选能够合成脂肪酸的微生物的方法是有用的。

6. 发明公开

EP2405018A9 METHOD FOR DETERMINING FATTY ACID SYNTHESIS PATHWAY OF MICROORGANISM, AND PCR PRIMER SET FOR USE IN THE METHOD 审中-公开
标题翻译：程序对测定微生物和PCR引物的脂肪酸合成PATH用于该方法
公开(公告)号：EP2405018A9
公开(公告)日：2012-03-14
申请号：EP10748811.6
申请日：2010-03-04
申请人： University of Miyazaki , Nippon Suisan Kaisha, Ltd. , Kyushu University, National University Corporation
发明人： NAGANO, Naoki , HAYASHI, Masahiro , SAKAGUCHI, Keishi , ITO, Makoto , TAOKA, Yosuke , OKITA, Yuji
IPC分类号： C12Q1/68 , C12N15/09
CPC分类号： C12Q1/689
摘要： Disclosed is a method for determining the fatty acid synthesis pathway of a microorganism. Specifically disclosed is a method for determining the fatty acid synthesis pathway of a microorganism, which is characterized by producing degenerate primers for a fatty acid synthesis-related enzyme gene and determining the presence or absence of the fatty acid synthesis-related gene in the genome gene extracted from the microorganism using the degenerate primers. The sequences for degenerate primers for C20-elongase gene, which is a fatty acid synthesis-related enzyme gene, are ATHGARTWYTKBRTITTYGTICA (SEQ ID NO:1) and TARTRISWRTACATIADIAMRTG (SEQ ID NO:2), and the sequences for degenerate primers for Δ4-desaturase gene are GGNCAYCAYCMITAYACNAA (SEQ ID NO:3) and TCDATYTGRTGIBWIARNCC (SEQ ID NO:4). The microorganism is one belonging to the class Labyrinthulea. Also specifically disclosed is a PCR primer set for use in the determination method. The method is useful as a method for screening a microorganism capable of synthesizing a fatty acid.

7. 发明公开

EP3318640A1 LABYRINTHULID MICROORGANISM CAPABLE OF PRODUCING MICROBIAL OIL, MICROBIAL OIL, METHODS RESPECTIVELY FOR PRODUCING SAID MICROORGANISM AND FOR PRODUCING SAID MICROBIAL OIL, AND USES OF SAID MICROORGANISM AND SAID MICROBIAL OIL 审中-公开
标题翻译：可用于生产微生物油，微生物油的微生物群落，分别用于产生所述微生物和生产所述微生物油的方法以及所述微生物和所述微生物油的用途
公开(公告)号：EP3318640A1
公开(公告)日：2018-05-09
申请号：EP16821387.4
申请日：2016-07-04
申请人： Kuysyu University, National University Corporation , Konan Gakuen , Nippon Suisan Kaisha, Ltd.
发明人： OKITA, Yuji , ITO, Makoto , HAMAGUCHI, Rie , GODA, Hatsumi , MOCHINAGA, Seiya , HONDA, Daisuke
IPC分类号： C12P7/64 , C12N1/13 , C12N15/09 , C12P1/00 , C12R1/89
CPC分类号： C12P7/6427 , C10G3/00 , C12N15/09 , C12N15/52 , C12N15/79 , C12P1/00 , C12P7/64 , C12P7/6472 , C12P19/34 , C12R1/00 , C12R1/89
摘要： [Problem] To provide a Labyrinthulid microorganism capable of producing a PUFA only through the elongase-desaturase pathway. [Solution] A Labyrinthulid microorganism which does not have an ability of producing a PUFA through the endogenous PUFA-PKS pathway or has the ability at an extremely weak level and which has an ability of producing a PUFA through the endogenous elongase-desaturase pathway. The Labyrinthulid microorganism is a microorganism belonging to the genus Parietichytrium or Parietichytrium
摘要翻译： [问题]提供仅通过延伸酶 - 去饱和酶途径能够产生PUFA的迷迭香属微生物。 [1]一种迷迭香属微生物，其不具有通过内源性PUFA-PKS途径产生PUFA的能力或具有极弱水平的能力并且具有通过内源性延伸酶 - 去饱和酶途径产生PUFA的能力。迷迭香属微生物是属于Parietichytrium或Parietichytrium属的微生物

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