专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明公开

EP0759465A1 GENE IMPARTING FLOCCULABILITY TO YEAST AND GENE PRODUCT 失效
标题翻译： DEF HEFE DIEFLOCKUNGSFÄHIGKEITVERLEIHENDES GEN UND GENPRODUKT
公开(公告)号：EP0759465A1
公开(公告)日：1997-02-26
申请号：EP96901494.3
申请日：1996-01-31
申请人： KIRIN BEER KABUSHIKI KAISHA
发明人： KOBAYASHI, Osamu, Kirin Beer Kabushiki Kaisha , HAYASHI, Nobuyuki Kirin Beer Kabushiki Kaisha , SONE, Hidetaka, Kirin Beer Kabushiki Kaisha
IPC分类号： C12N15/11 , C12N15/31 , C12N15/62 , C12P21/02 , C12N1/19 , C07K14/395 , C12C11/02
CPC分类号： C07K14/395
摘要： The present invention relates to a protein having an activity of conferring on yeast brewer's yeast-type flocculating property; a DNA coding for the protein; a plasmid containing the DNA; a method for producing a yeast strain wherein brewer's yeast-type flocculating property has been conferred or enhanced with the use of the DNA and a method for producing a yeast strain wherein brewer's yeast-type flocculating property has been eliminated or reduced with the use of the DNA; and a method for eliminating or reducing brewer's yeast-type flocculating property of yeast by inhibiting the expression of the DNA.
The present invention also relates to a yeast strain wherein brewer's yeast-type flocculating property has been conferred, enhanced, eliminated or reduced by the above method.
Further, the present invention relates to a method for producing a brewed product comprising culturing the above yeast strain as well as a brewed product obtained by the method.
According to the present invention, there are provided the Lg-Flo1 protein having an activity of conferring on yeast brewer's yeast-type flocculating property and the DNA strand of Lg- FLO1 coding for the protein. By introducing the above DNA of the invention into yeast cells as a foreign gene by genetic engineering techniques, i.e., introducing the DNA into yeast cells as an extranuclear gene and(/or) a nuclear gene, it is possible to confer on yeast brewer's yeast-type flocculating property or enhance this property in yeast. On the contrary, by introducing a DNA is obtained by destroying the DNA of the invention into yeast cells, or by inhibiting the expression of the DNA of the invention, it is possible to convert a flocculent yeast strain into a non-flocculent strain, or to reduce flocculating property.
摘要翻译：本发明涉及具有赋予酵母酿酒酵母型絮凝性的活性的蛋白质，编码蛋白质的DNA; 含有DNA的质粒; 利用DNA赋予或增强了啤酒酵母型絮凝性的酵母菌株的制造方法以及利用该酵母菌种的啤酒酵母型絮凝性已被消除或减少的酵母菌株的制造方法脱氧核糖核酸; 以及通过抑制DNA的表达来消除或减少酵母的酵母型絮凝特性的方法。本发明还涉及通过上述方法赋予，增强，消除或减少啤酒酵母型絮凝性的酵母菌株。此外，本发明涉及一种生产酿造产品的方法，包括培养上述酵母菌株以及通过该方法获得的酿造产品。根据本发明，提供具有赋予酵母酿酒酵母型絮凝性的活性的Lg-Flo1蛋白质和编码该蛋白质的Lg-FLO1的DNA链。通过基因工程技术将本发明的上述DNA引入作为外源基因的酵母细胞，即将DNA引入作为核外基因的酵母细胞和（/或）核基因，可以赋予酵母酿酒酵母型絮凝性或提高酵母中的这一性质。相反，通过将通过将本发明的DNA破坏成酵母细胞获得的DNA或通过抑制本发明的DNA的表达获得，可以将絮状酵母菌株转化为非絮状菌株，或以减少絮凝性。

IPRDB

热门服务

关于我们

友情链接

联系方式