专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明公开

EP1352958A1 GENE DISRUPTED YEAST 审中-公开
标题翻译： HEFE MIT UNTERBROCHENEM GEN
公开(公告)号：EP1352958A1
公开(公告)日：2003-10-15
申请号：EP02716318.7
申请日：2002-01-18
申请人： KANEKA CORPORATION
发明人： FUKUCHI, Ken , YOKOMIZO, Satoru , OSAKADA, Fumio , MATSUMOTO, Keiji , TAKAGI, Masamichi , OTA, Akinori
IPC分类号： C12N15/09 , C12N1/19 , C12P7/62
CPC分类号： C12N9/93 , C12P7/625 , C12Y603/02006
摘要： The invention provides a yeast in which a specific gene alone has been disrupted and to which auxotrophy is impared to construct a system for producing an industrially useful substance through gene recombination by utilizing characteristics of a yeast.
In the practice of the invention, a yeast strain in which ADE1 gene has been disrupted is constructed by homologous recombination with a chromosomal DNA by using an ADE1 gene fragment encoding phosphoribosylaminoimidazole succinocarboxamide synthase (EC6.3.2.6) of Candida maltosa. In the practice of the invention, then, this disrupted strain is transformed by a gene expression cassette containing a biodegradable polyester synthase gene and the obtained transformant is cultured to thereby produce biodegradable polyester.
摘要翻译：本发明提供了一种酵母，其中单独的特定基因已经被破坏并且通过利用酵母的特性通过基因重组构建了用于产生工业上有用物质的制剂的营养缺陷型。在本发明的实践中，通过使用编码拟南芥假丝酵母的磷酸核糖氨基咪唑琥珀酰胺合成酶（EC6.3.2.6）的ADE1基因片段，通过与染色体DNA的同源重组构建其中ADE1基因被破坏的酵母菌株。在本发明的实践中，通过含有可生物降解的聚酯合酶基因的基因表达盒转化该破坏的菌株，培养得到的转化体，从而生成可生物降解的聚酯。

IPRDB

热门服务

关于我们

友情链接

联系方式