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1. 发明公开

EP0324157A1 Gene fragment coding for a rabies virus glycoprotein and process for producing a rabies virus glycoprotein using the same 失效
标题翻译：该基因编码的狂犬病毒，以及该基因的方法制备这种糖蛋白的方法的糖蛋白的片段。
公开(公告)号：EP0324157A1
公开(公告)日：1989-07-19
申请号：EP88121602.2
申请日：1988-12-23
申请人： Juridical Foundation The Chemo-Sero-Therapeutic Research Institute
发明人： Sakamoto, Shinichi , Ide, Toshio , Tokiyoshi, Sachio , Yamamoto, Michitaka
IPC分类号： C12N15/00 , A61K39/205 , C12P21/00
CPC分类号： C07K14/005 , A61K39/00 , C12N2760/20122
摘要： A gene fragment is described which encodes a rabies virus glycoprotein. Furthermore, a recombinant rabies virus glycoprotein expressed by using said gene fragment which is extremely useful for preparing rabies vaccines and diagnostic reagents and a process for preparing a rabies virus glycoprotein are described which process comprises introducing into eukaryotic cells a shuttle vector wherein the gene fragment coding for a rabies virus glycoprotein is inserted downstream of a promoter capable of functioning in eukaryotic cells, and culturing said transformed cells to yield a rabies virus glycoprotein in the cytoplasm of said cells. Since the G protein can be expressed in the cytoplasm as a granule, not coupled to the cell membrane, the process of this invention can give the desired G protein in high yields and high purity. The G protein of this invention can not only react with antibodies against the G protein but also induces antibodies having an activity of neutralizing rabies virus when injected into animals.
摘要翻译：的基因片段描述编码狂犬病毒糖蛋白。进一步，通过使用所述基因片段的所有其是用于制备狂犬病疫苗和诊断试剂以及用于制备狂犬病毒糖蛋白的方法是非常有用的过表达的重组狂犬病毒糖蛋白中描述，该方法包括引入到真核细胞的穿梭载体worin基因片段编码用于狂犬病毒糖蛋白被插入能够在真核细胞中起作用，并培养所述的转化细胞中的细胞。所述的细胞质中产生狂犬病毒糖蛋白的启动子的下游。由于G蛋白可在细胞质中过表达。颗粒剂，没有耦合到细胞膜中，本发明的方法可以以高产率和高纯度得到所需的G蛋白。本发明的G蛋白不仅能与针对G蛋白的抗体，从而发生反应，但诱导具有中和狂犬病毒的活性的抗体当注射入动物。

2. 发明授权

EP0522535B1 Recombinant marek's disease virus, process for preparing the same and vaccine containing the same 失效
标题翻译：马立克氏病的重组病毒，方法使得这种病毒和含有这种疫苗
公开(公告)号：EP0522535B1
公开(公告)日：2003-04-16
申请号：EP92111588.7
申请日：1992-07-08
申请人： Juridical Foundation The Chemo-Sero-Therapeutic Research Institute
发明人： Sakaguchi, Masashi , Yamamoto, Michitaka
IPC分类号： C07K2/00 , C12N15/38 , C12N15/86 , C12P21/00 , A61K39/255
CPC分类号： C12N15/86 , A61K39/00 , C07K14/005 , C07K2319/00 , C07K2319/40 , C07K2319/61 , C12N2710/16322 , C12N2710/16343 , C12N2760/18122

3. 发明授权

EP0361182B1 Recombinant Marek's disease virus, process for preparing the same and vaccine containing the same 失效
标题翻译：重组马立克氏病病毒，其制备方法和疫苗。
公开(公告)号：EP0361182B1
公开(公告)日：1994-01-19
申请号：EP89116679.5
申请日：1989-09-08
申请人： Juridical Foundation The Chemo-Sero-Therapeutic Research Institute
发明人： Sakaguchi, Masashi , Maeda, Hiroaki , Yamamoto, Michitaka , Miyazaki, Junichi Higashimachi Minami-jutaku 3-101
IPC分类号： C12N15/86 , C12N15/85 , A61K39/255
CPC分类号： C12N15/86 , A61K39/12 , A61K39/255 , C07K14/005 , C12N2710/16322 , C12N2710/16334 , C12N2710/16343

4. 发明公开

EP0522535A1 Recombinant marek's disease virus, process for preparing the same and vaccine containing the same 失效
标题翻译：马立克氏病的重组病毒，用于制作该病毒与那些含有疫苗的方法。
公开(公告)号：EP0522535A1
公开(公告)日：1993-01-13
申请号：EP92111588.7
申请日：1992-07-08
申请人： Juridical Foundation The Chemo-Sero-Therapeutic Research Institute
发明人： Sakaguchi, Masashi , Yamamoto, Michitaka
IPC分类号： C07K15/00 , C12N15/38 , C12N15/86 , C12P21/00 , A61K39/255
CPC分类号： C12N15/86 , A61K39/00 , C07K14/005 , C07K2319/00 , C07K2319/40 , C07K2319/61 , C12N2710/16322 , C12N2710/16343 , C12N2760/18122
摘要： A recombinant Marek's disease virus produced by the mutation of a Marek's disease virus with a plasmid wherein said plasmid comprises (1) a gene fragment derived from the Us region or inverted repeat sequences adjacent to both ends of said Us region of a Marek's disease virus genome and (2) an exogenous gene expression cassette incorporated in said gene fragment, said cassette comprising an exogenous gene bound downstream of a promoter derived from an animal cell or an animal virus, a process for preparing the same, a multivalent live vaccine for birds comprising the same, and a vector for administration of a physiologically active substance to birds which comprises the same.
摘要翻译：由马立克氏病病毒的与质粒的突变产生的重组马立克氏病病毒worin所述质粒包括：（1）从Us区域或反向重复序列邻近于马立克氏病病毒基因组的说，美国区域的两端来源的基因片段和（2）在所述基因片段掺入外源基因表达盒的，所述盒子外源基因包含结合从动物细胞或动物病毒，其制备方法，多价活疫苗的鸟类的方法，包括的启动子的下游同样的，和生理活性物质施用给鸟类，包括相同的向量。

5. 发明公开

EP0361182A1 Recombinant Marek's disease virus, process for preparing the same and vaccine containing the same 失效
标题翻译： Rekombinantes Marek-Krankheitsvirus，Verfahren zu seiner Herstellung，und Vakzine。
公开(公告)号：EP0361182A1
公开(公告)日：1990-04-04
申请号：EP89116679.5
申请日：1989-09-08
申请人： Juridical Foundation The Chemo-Sero-Therapeutic Research Institute
发明人： Sakaguchi, Masashi , Maeda, Hiroaki , Yamamoto, Michitaka , Miyazaki, Junichi Higashimachi Minami-jutaku 3-101
IPC分类号： C12N15/86 , C12N15/85 , A61K39/255
CPC分类号： C12N15/86 , A61K39/12 , A61K39/255 , C07K14/005 , C12N2710/16322 , C12N2710/16334 , C12N2710/16343
摘要： A recombinant Marek's disease is described which comprises a Marek's disease virus genome and a DNA fragment incorporated therein, said DNA fragment containing
a promoter derived from an animal cell or an animal virus and a structural gene coding for an exogenous protein. Furthermore a process for preparing said recombinant virus is described which comprises
preparing a gene fragment wherein a structural gene coding for an exogenous gene is located downstream of a promoter derived from an animal cell or an animal virus, incorporating said gene fragment into theBamHI - H fragment of a gene of a Marek's disease virus type I, and incorporating said fragment into a Marek's disease virus type I genome. Finally a multivalent live vaccine for birds is described which contains the above recombinant virus.
摘要翻译：描述了重组马立克氏病，其包含马立克氏病病毒基因组和其中掺入的DNA片段，所述DNA片段含有源自动物细胞或动物病毒的启动子和编码外源蛋白质的结构基因。此外，描述了制备所述重组病毒的方法，其包括制备基因片段，其中编码外源基因的结构基因位于来源于动物细胞或动物病毒的启动子的下游，将所述基因片段并入BHIHI-H片段的马立克氏病病毒I型的基因，并将所述片段并入马立克氏病病毒I型基因组。最后描述了含有上述重组病毒的鸟类多价活疫苗。

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