专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明授权

EP0778351B1 Method of fingerprinting polynucleotides 失效
标题翻译：一种用于多核苷酸的指纹识别方法
公开(公告)号：EP0778351B1
公开(公告)日：2002-08-28
申请号：EP96118921.4
申请日：1996-11-26
申请人： Hitachi, Ltd.
发明人： Kambara, Hideki , Okano, Kazunori , Uematsu, Chihiro
IPC分类号： C12Q1/68 , B01L7/00
CPC分类号： C12Q1/6855 , C12Q1/683

2. 发明授权

EP0649852B1 Fractionation method for nucleotide fragments 失效
标题翻译：核苷酸片段的Fraktioniermethode
公开(公告)号：EP0649852B1
公开(公告)日：2000-01-05
申请号：EP94116671.2
申请日：1994-10-21
申请人： HITACHI, LTD.
发明人： Kambara, Hideki , Okano, Kazunori , Uematsu, Chihiro
IPC分类号： C07H1/06 , C12Q1/68
CPC分类号： G01N27/44743 , C12Q1/6837 , C12Q1/6874 , G01N27/447 , C12Q2535/101 , C12Q2525/179 , C12Q2565/537 , C12Q2525/191

3. 发明公开

EP0778351A3 Method of analysis or assay for polynucleotides and analyzer or instrument for polynucleotides 失效
标题翻译：一种用于分析或检测多核苷酸和分析系统或仪器多核苷酸的方法
公开(公告)号：EP0778351A3
公开(公告)日：1999-01-20
申请号：EP96118921.4
申请日：1996-11-26
申请人： HITACHI, LTD.
发明人： Kambara, Hideki , Okano, Kazunori , Uematsu, Chihiro
IPC分类号： C12Q1/68 , B01L7/00
CPC分类号： C12Q1/6855 , C12Q1/683
摘要： A method of analysis or assay for nucleotides comprises:
(1) a step of digesting DNA with a restriction enzyme; (2) a step of discriminating a difference in sequences of the DNA fragments obtained in step (1) above around the 3' termini thereof with a DNA probe and extending the DNA probe by a complementary strand synthesis to fractionate the DNA fragments into groups; and, (3) a step of measuring lengths of the DNA fragments which belong to said groups, or length of the DNA probe extended by said complementary strand extension reaction;
wherein the thus measured lengths obtained for every sequence of the bases of the DNA fragments around the 3' termini thereof are employed as fingerprints.

4. 发明公开

EP0778351A2 Method of analysis or assay for polynucleotides and analyzer or instrument for polynucleotides 失效
标题翻译： Verfahren zur Analyze oder Nachweis von Polynukleotiden und Analysesystem oder InstrumentfürPolynuklotide
公开(公告)号：EP0778351A2
公开(公告)日：1997-06-11
申请号：EP96118921.4
申请日：1996-11-26
申请人： HITACHI, LTD.
发明人： Kambara, Hideki , Okano, Kazunori , Uematsu, Chihiro
IPC分类号： C12Q1/68 , B01L7/00
CPC分类号： C12Q1/6855 , C12Q1/683
摘要： A method of analysis or assay for nucleotides comprises:

(1) a step of digesting DNA with a restriction enzyme;
(2) a step of discriminating a difference in sequences of the DNA fragments obtained in step (1) above around the 3' termini thereof with a DNA probe and extending the DNA probe by a complementary strand synthesis to fractionate the DNA fragments into groups; and,
(3) a step of measuring lengths of the DNA fragments which belong to said groups, or length of the DNA probe extended by said complementary strand extension reaction;
wherein the thus measured lengths obtained for every sequence of the bases of the DNA fragments around the 3' termini thereof are employed as fingerprints.
摘要翻译：核苷酸的分析或测定方法包括：（1）用限制酶消化DNA的步骤; （2）通过DNA探针区分3'末端的步骤（1）中获得的DNA片段的序列差异的步骤，并通过互补链合成扩增DNA探针，将DNA片段分馏成组; 和（3）测量属于所述组的DNA片段的长度或由所述互补链延伸反应延伸的DNA探针的长度的步骤; 其中将由此测得的长度为3'末端周围的DNA片段的碱基的每个序列获得的长度作为指纹。

5. 发明公开

EP0649852A1 Fractionation method for nucleotide fragments 失效
标题翻译： FraktioniermethodefürNukleotidfragmente。
公开(公告)号：EP0649852A1
公开(公告)日：1995-04-26
申请号：EP94116671.2
申请日：1994-10-21
申请人： HITACHI, LTD.
发明人： Kambara, Hideki , Okano, Kazunori , Uematsu, Chihiro
IPC分类号： C07H1/06 , C12Q1/68
CPC分类号： G01N27/44743 , C12Q1/6837 , C12Q1/6874 , G01N27/447 , C12Q2535/101 , C12Q2525/179 , C12Q2565/537 , C12Q2525/191
摘要： A fractionation method for DNA fragments according to the present invention comprises a first step of preparing a probe chip or a set of probe chips immobilizing independently a DNA probe having a first sequence part having a specific known sequence part together with a part of enzyme recognition sequence and a second sequence part composed of a combination of one to six bases adjacent to the first sequence part at 3' terminus, a second step of introducing a DNA oligomer composed of a part of enzyme recognition sequence and a sequence complementary to the known sequence part into the fragment termini of DNA fragments from restriction enzyme cleavage, and a third step of placing the probe chip or the set of probe chips in a solution containing the nucleotide fragments with the introduced DNA oligomer produced at the second step, for at least hybridization and the complementary strand extension of the DNA probe, whereby the DNA fragments are fractionated.
摘要翻译：根据本发明的DNA片段的分级方法包括制备探针芯片或一组探针芯片的第一步骤，其独立地固定有具有特定已知序列部分的第一序列部分的DNA探针以及酶识别序列的一部分和由3'末端与第一序列部分相邻的1〜6个碱基的组合构成的第2序列部分，第2步骤，导入由部分酶识别序列构成的DNA寡聚物和与已知序列部分互补的序列进入限制酶切割的DNA片段的片段末端，以及第三步骤，将探针片或该组探针片放置在含有第二步产生的引入的DNA寡聚物的核苷酸片段的溶液中至少杂交和 DNA探针的互补链延伸，从而分离DNA片段。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式