专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明公开

EP2316963A1 Combinatorial DNA library for producing modified N-glycans in lower eukaryotes 有权
标题翻译：制造的组合DNA文库修饰的N-葡聚糖在低等真核生物
公开(公告)号：EP2316963A1
公开(公告)日：2011-05-04
申请号：EP10013052.5
申请日：2004-02-20
申请人： GlycoFi, Inc.
发明人： Gerngross, Tillman U. , Wildt, Stefan , Choi, Byung-Kwon , Nett, Juergen Hermann , Bobrowicz, Piotr , Hamilton, Stephen R. , Davidson, Robert C.
IPC分类号： C12P21/00 , C12N9/10 , C12N9/24 , C12N1/14 , C12N5/10 , C12R1/645
CPC分类号： C12P21/005 , C07K14/47 , C12N9/1051 , C12N15/1082 , C12N15/79
摘要： The present invention relates to eukaryotic host cells having modified oligosaccharides which may be modified further by heterologous expression of a set of glycosyltransferases, sugar transporters and mannosidases to become host-strains for the production of mammalian, e.g., human therapeutic glycoproteins. The invention provides nucleic acid molecules and combinatorial libraries which can be used to successfully target and express mammalian enzymatic activities such as those involved in glycosylation to intracellular compartments in a eukaryotic host cell. The process provides an engineered host cell which can be used to express and target any desirable gene(s) involved in glycosylation. Host cells with modified oligosaccharides are created or selected. N-glycans made in the engineered host cells have a Man5GlcNAc2 core structure which may then be modified further by heterologous expression of one or more enzymes, e.g., glycosyltransferases, sugar transporters and mannosidases, to yield human-like glycoproteins. For the production of therapeutic proteins, this method may be adapted to engineer cell lines in which any desired glycosylation structure may be obtained.

2. 发明授权

EP1597381B2 PRODUCTION OF MODIFIED GLYCOPROTEINS HAVING MULTIPLE ANTENNARY STRUCTURES 有权转让
标题翻译：生产改性糖蛋白多分支结构的
公开(公告)号：EP1597381B2
公开(公告)日：2013-05-15
申请号：EP04713388.9
申请日：2004-02-20
申请人： GlycoFi, Inc.
发明人： Bobrowicz, Piotr , Hamilton, Stephen R. , Gerngross, Tillman U. , Wildt, Stefan , Choi, Byung-Kwon , Nett, Juergen Hermann , Davidson, Robert C.
IPC分类号： C12P21/00 , C12N9/10 , C12N5/10 , C12N15/81 , C12R1/84
CPC分类号： C07K14/39 , A61K38/00 , C07K2319/05 , C12N9/1051 , C12P21/005 , Y02A50/396
摘要： The present invention relates to eukaryotic host cells, especially lower eukaryotic host cells, having modified oligosaccharides which may be modified further by heterologous expression of a set of glycosyltransferases, sugar and sugar nucleotide transporters to become host-strains for the production of mammalian, e.g., human therapeutic glycoproteins. The process provides an engineered host cell which can be used to express and target any desirable gene(s) involved in glycosylation. Host cells with modified lipid-linked oligosaccharides are created or selected. N-glycans made in the engineered host cells exhibit GnTIII, GnTIV, GnTV, GnT VI or GnTIX activity, which produce bisected and/or multiantennary N-glycan structures and may be modified further by heterologous expression of one or more enzymes, e.g., glycosyltransferases, sugar, sugar nucleotide transporters, to yield human-like glycoproteins. For the production of therapeutic proteins, this method may be adapted to engineer cell lines in which any desired glycosylation structure may be obtained.

3. 发明授权

EP2316963B1 Combinatorial DNA library for producing modified N-glycans in lower eukaryotes 有权
标题翻译：制造的组合DNA文库修饰的N-葡聚糖在低等真核生物
公开(公告)号：EP2316963B1
公开(公告)日：2013-04-10
申请号：EP10013052.5
申请日：2004-02-20
申请人： GlycoFi, Inc.
发明人： Gerngross, Tillman U. , Wildt, Stefan , Choi, Byung-Kwon , Nett, Juergen Hermann , Bobrowicz, Piotr , Hamilton, Stephen R. , Davidson, Robert C.
IPC分类号： C12P21/00 , C12N9/10 , C12N9/24 , C12N1/14 , C12N5/10 , C12R1/645
CPC分类号： C12P21/005 , C07K14/47 , C12N9/1051 , C12N15/1082 , C12N15/79

4. 发明授权

EP2080809B1 Production of modified glycoproteins having multiple antennary structures 有权
标题翻译：具有结构multiantennären改性的糖蛋白的生产
公开(公告)号：EP2080809B1
公开(公告)日：2012-02-01
申请号：EP09004760.6
申请日：2004-02-20
申请人： GlycoFi, Inc.
发明人： Bobrowicz, Piotr , Hamilton, Stephan R. , Gerngross, Tillman U. , Wildt, Stefhan , Choi, Byung-Kwon , Nett, Juergen Hermann , Davidson, Robert C.
IPC分类号： C12P21/00 , C12N9/10 , C12N5/10 , C12N15/81 , C12R1/84
CPC分类号： C07K14/39 , A61K38/00 , C07K2319/05 , C12N9/1051 , C12P21/005 , Y02A50/396
摘要： The present invention relates to eukaryotic host cells, especially lower eukaryotic host cells, having modified oligosaccharides which may be modified further by heterologous expression of a set of glycosyltransferases, sugar and sugar nucleotide transporters to become host-strains for the production of mammalian, e.g., human therapeutic glycoproteins. The process provides an engineered host cell which can be used to express and target any desirable gene(s) involved in glycosylation. Host cells with modified lipid-linked oligosaccharides are created or selected. N-glycans made in the engineered host cells exhibit GnTIII, GnTIV, GnTV, GnT VI or GnTIX activity, which produce bisected and/or multiantennary N-glycan structures and may be modified further by heterologous expression of one or more enzymes, e.g., glycosyltransferases, sugar, sugar nucleotide transporters, to yield human-like glycoproteins. For the production of therapeutic proteins, this method may be adapted to engineer cell lines in which any desired glycosylation structure may be obtained.

5. 发明公开

EP2196540A3 Combinatorial DNA library for producing modified N-Glycans in lower eukaryotes 有权
标题翻译：制造的组合DNA文库修饰的N-葡聚糖在低等真核生物
公开(公告)号：EP2196540A3
公开(公告)日：2010-09-22
申请号：EP10158532.1
申请日：2004-02-20
申请人： GlycoFi, Inc.
发明人： Gerngross, Tillman U. , Wildt, Stefan , Choi, Byung-Kwon , Nett, Juergen Hermann , Bobrowicz, Piotr , Hamilton, Stephen R. , Davidson, Robert C.
IPC分类号： C12P21/00 , C12N9/10 , C12N9/24 , C12N1/14 , C12N5/10 , C12R1/645
CPC分类号： C12P21/005 , C07K14/47 , C12N9/1051 , C12N15/1082 , C12N15/79
摘要： The present invention relates to eukaryotic host cells having modified oligosaccharides which may be modified further by heterologous expression of a set of glycosyltransferases, sugar transporters and mannosidases to become host-strains for the production of mammalian, e.g., human therapeutic glycoproteins. The invention provides nucleic acid molecules and combinatorial libraries which can be used to successfully target and express mammalian enzymatic activities such as those involved in glycosylation to intracellular compartments in a eukaryotic host cell. The process provides an engineered host cell which can be used to express and target any desirable gene(s) involved in glycosylation. Host cells with modified oligosaccharides are created or selected. N-glycans made in the engineered host cells have a Man5GlcNAc2 core structure which may then be modified further by heterologous expression of one or more enzymes, e.g., glycosyltransferases, sugar transporters and mannosidases, to yield human-like glycoproteins. For the production of therapeutic proteins, this method may be adapted to engineer cell lines in which any desired glycosylation structure may be obtained.

6. 发明公开

EP2196540A2 Combinatorial DNA library for producing modified N-Glycans in lower eukaryotes 有权
标题翻译：在Niederen Eukaryoten中的Kombinatorische DNA-Bibliothek zur Herstellung modifizierter N-Glukane
公开(公告)号：EP2196540A2
公开(公告)日：2010-06-16
申请号：EP10158532.1
申请日：2004-02-20
申请人： GlycoFi, Inc.
发明人： Gerngross, Tillman U. , Wildt, Stefan , Choi, Byung-Kwon , Nett, Juergen Hermann , Bobrowicz, Piotr , Hamilton, Stephen R. , Davidson, Robert C.
IPC分类号： C12P21/00 , C12N9/10 , C12N9/24 , C12N1/14 , C12N5/10 , C12R1/645
CPC分类号： C12P21/005 , C07K14/47 , C12N9/1051 , C12N15/1082 , C12N15/79
摘要： The present invention relates to eukaryotic host cells having modified oligosaccharides which may be modified further by heterologous expression of a set of glycosyltransferases, sugar transporters and mannosidases to become host-strains for the production of mammalian, e.g., human therapeutic glycoproteins. The invention provides nucleic acid molecules and combinatorial libraries which can be used to successfully target and express mammalian enzymatic activities such as those involved in glycosylation to intracellular compartments in a eukaryotic host cell. The process provides an engineered host cell which can be used to express and target any desirable gene(s) involved in glycosylation. Host cells with modified oligosaccharides are created or selected. N-glycans made in the engineered host cells have a Man5GlcNAc2 core structure which may then be modified further by heterologous expression of one or more enzymes, e.g., glycosyltransferases, sugar transporters and mannosidases, to yield human-like glycoproteins. For the production of therapeutic proteins, this method may be adapted to engineer cell lines in which any desired glycosylation structure may be obtained.
摘要翻译：本发明涉及具有修饰的寡糖的真核宿主细胞，其可以通过异源表达一组糖基转移酶，糖转运体和甘露糖苷酶进一步修饰，以成为用于产生哺乳动物例如人治疗性糖蛋白的宿主菌株。本发明提供了可用于成功靶向和表达哺乳动物酶活性的核酸分子和组合文库，例如参与真核宿主细胞中细胞内区室糖基化的那些酶活性。该方法提供了可用于表达和靶向参与糖基化的任何所需基因的工程化宿主细胞。建立或选择具有修饰的寡糖的宿主细胞。在工程化的宿主细胞中制备的N-聚糖具有Man5GlcNAc2核心结构，然后可以通过异源表达一种或多种酶，例如糖基转移酶，糖转运蛋白和甘露糖苷酶来进一步修饰，以产生人样糖蛋白。为了生产治疗性蛋白质，该方法可适用于工程化可能获得任何所需糖基化结构的细胞系。

7. 发明授权

EP1599595B1 N -ACETYLGLUCOSAMINYLTRANSFERASE III EXPRESSION IN LOWER EUKARYOTES 有权转让
标题翻译： N-乙酰III表达在低等真核生物
公开(公告)号：EP1599595B1
公开(公告)日：2009-11-25
申请号：EP04713412.7
申请日：2004-02-20
申请人： GlycoFi, Inc.
发明人： Bobrowicz, Piotr , Hamilton, Stephen R. , Gerngross, Tillman U , Wildt, Stefan , Choi, Byung-Kwon , Nett, Juergen Hermann , Davidson, Robert C
IPC分类号： C12P21/00 , C12N9/10 , C12N5/10 , C12N15/81 , C12R1/645
CPC分类号： C07K14/39 , A61K38/00 , C07K2319/05 , C12N9/1051 , C12P21/005 , Y02A50/396
摘要： The present invention relates to eukaryotic host cells, especially lower eukaryotic host cells, having modified oligosaccharides which may be modified further by heterologous expression of a set of glycosyltransferases, sugar and sugar nucleotide transporters to become host-strains for the production of mammalian, e.g., human therapeutic glycoproteins. The process provides an engineered host cell which can be used to express and target any desirable gene(s) involved in glycosylation. Host cells with modified lipid-linked oligosaccharides are created or selected. N-glycans made in the engineered host cells exhibit GnTIII, GnTIV, GnTV, GnT VI or GnTIX activity, which produce bisected and/or multiantennary N-glycan structures and may be modified further by heterologous expression of one or more enzymes, e.g., glycosyltransferases, sugar, sugar nucleotide transporters, to yield human-like glycoproteins. For the production of therapeutic proteins, this method may be adapted to engineer cell lines in which any desired glycosylation structure may be obtained.

8. 发明公开

EP2080809A1 Production of modified glycoproteins having multiple antennary structures 有权
标题翻译： Herstellung modifizierter Glykoproteine mitmultiantennärenStrukturen
公开(公告)号：EP2080809A1
公开(公告)日：2009-07-22
申请号：EP09004760.6
申请日：2004-02-20
申请人： GlycoFi, Inc.
发明人： Bobrowicz, Piotr , Hamilton, Stephan R. , Gerngross, Tillman U. , Wildt, Stefhan , Choi, Byung-Kwon , Nett, Juergen Hermann , Davidson, Robert C.
IPC分类号： C12P21/00 , C12N9/10 , C12N5/10 , C12N15/81 , C12R1/84
CPC分类号： C07K14/39 , A61K38/00 , C07K2319/05 , C12N9/1051 , C12P21/005 , Y02A50/396
摘要： The present invention relates to eukaryotic host cells, especially lower eukaryotic host cells, having modified oligosaccharides which may be modified further by heterologous expression of a set of glycosyltransferases, sugar and sugar nucleotide transporters to become host-strains for the production of mammalian, e.g., human therapeutic glycoproteins. The process provides an engineered host cell which can be used to express and target any desirable gene(s) involved in glycosylation. Host cells with modified lipid-linked oligosaccharides are created or selected. N-glycans made in the engineered host cells exhibit GnTIII, GnTIV, GnTV, GnT VI or GnTIX activity, which produce bisected and/or multiantennary N-glycan structures and may be modified further by heterologous expression of one or more enzymes, e.g., glycosyltransferases, sugar, sugar nucleotide transporters, to yield human-like glycoproteins. For the production of therapeutic proteins, this method may be adapted to engineer cell lines in which any desired glycosylation structure may be obtained.
摘要翻译：本发明涉及具有修饰的寡糖的真核宿主细胞，特别是低等真核宿主细胞，其可以进一步通过异源表达一组糖基转移酶，糖和糖核苷酸转运蛋白进行修饰，以成为用于产生哺乳动物的宿主菌株，人类治疗糖蛋白。该方法提供了可用于表达和靶向参与糖基化的任何所需基因的工程化宿主细胞。产生或选择具有修饰的脂质连接寡糖的宿主细胞。在工程化宿主细胞中制备的N-聚糖表现出GnTIII，GnTIV，GnTV，GnT VI或GnTIX活性，其产生二等分和/或多元N-聚糖结构，并且可以通过异源表达一种或多种酶，例如糖基转移酶，糖，糖核苷酸转运蛋白，以产生人样糖蛋白。为了生产治疗性蛋白质，该方法可适用于工程化可能获得任何所需糖基化结构的细胞系。

9. 发明授权

EP1599595B2 N -ACETYLGLUCOSAMINYLTRANSFERASE III EXPRESSION IN LOWER EUKARYOTES 有权转让
标题翻译： NEDA乙酰胆碱酯酶III在NIEDEREN EUKARYOTEN中的表达
公开(公告)号：EP1599595B2
公开(公告)日：2013-08-21
申请号：EP04713412.7
申请日：2004-02-20
申请人： GlycoFi, Inc.
发明人： Bobrowicz, Piotr , Hamilton, Stephen R. , Gerngross, Tillman U , Wildt, Stefan , Choi, Byung-Kwon , Nett, Juergen Hermann , Davidson, Robert C
IPC分类号： C12P21/00 , C12N9/10 , C12N5/10 , C12N15/81 , C12R1/645
CPC分类号： C07K14/39 , A61K38/00 , C07K2319/05 , C12N9/1051 , C12P21/005 , Y02A50/396
摘要： The present invention relates to eukaryotic host cells, especially lower eukaryotic host cells, having modified oligosaccharides which may be modified further by heterologous expression of a set of glycosyltransferases, sugar and sugar nucleotide transporters to become host-strains for the production of mammalian, e.g., human therapeutic glycoproteins. The process provides an engineered host cell which can be used to express and target any desirable gene(s) involved in glycosylation. Host cells with modified lipid-linked oligosaccharides are created or selected. N-glycans made in the engineered host cells exhibit GnTIII, GnTIV, GnTV, GnT VI or GnTIX activity, which produce bisected and/or multiantennary N-glycan structures and may be modified further by heterologous expression of one or more enzymes, e.g., glycosyltransferases, sugar, sugar nucleotide transporters, to yield human-like glycoproteins. For the production of therapeutic proteins, this method may be adapted to engineer cell lines in which any desired glycosylation structure may be obtained.

10. 发明授权

EP2196540B1 Combinatorial DNA library for producing modified N-Glycans in lower eukaryotes 有权
标题翻译：制造的组合DNA文库修饰的N-葡聚糖在低等真核生物
公开(公告)号：EP2196540B1
公开(公告)日：2012-07-04
申请号：EP10158532.1
申请日：2004-02-20
申请人： GlycoFi, Inc.
发明人： Gerngross, Tillman U. , Wildt, Stefan , Choi, Byung-Kwon , Nett, Juergen Hermann , Bobrowicz, Piotr , Hamilton, Stephen R. , Davidson, Robert C.
IPC分类号： C12P21/00 , C12N9/10 , C12N9/24 , C12N1/14 , C12N5/10 , C12R1/645
CPC分类号： C12P21/005 , C07K14/47 , C12N9/1051 , C12N15/1082 , C12N15/79

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式