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1. 发明公开

EP2627449A2 REAL-TIME AMPLIFICATION AND MICRO-ARRAY BASED DETECTION OF NUCLEIC ACID TARGETS IN A FLOW CHIP ASSAY 审中-公开
标题翻译： NUCLEIC靶区在河里芯片分析ECHTZEITVERSTÄRKUNGS-和微阵列，基于视觉的检测
公开(公告)号：EP2627449A2
公开(公告)日：2013-08-21
申请号：EP11764750.3
申请日：2011-10-06
申请人： Eppendorf AG
发明人： ALEXANDRE, Isabelle , DEROECK, Sven , REMACLE, Jose , PLUESTER, Wilhelm
IPC分类号： B01L7/00 , B01L3/00 , G01N21/64 , C12Q1/68
CPC分类号： C12Q1/6837 , B01L3/502784 , B01L7/525 , B01L2300/0636 , B01L2300/0816 , B01L2300/088 , B01L2300/0883 , C12Q1/6851 , C12Q1/6876 , C12Q2561/113 , C12Q2565/629
摘要： The present method is related to a method for identification and/or quantification of at least one polynucleotide target compound present in a biological sample among possible other ones by its amplification in a cycling flow chip solution passing through different temperatures required for the amplification and its detection in real-time onto a micro-array of specific capture molecules.

2. 发明公开

EP2389576A1 DETECTION AND/OR QUANTIFICATION METHOD OF TARGET MOLECULES ON A SOLID SUPPORT 审中-公开
标题翻译：检测和/或定量方法靶分子固相载体上
公开(公告)号：EP2389576A1
公开(公告)日：2011-11-30
申请号：EP09786297.3
申请日：2009-01-21
申请人： EPPENDORF AG
发明人： ALEXANDRE, Isabelle , REMACLE, Jose , KOEHN, Heinz
IPC分类号： G01N21/64 , C12Q1/68
CPC分类号： G01N21/6452 , C12Q1/6825 , G01N21/648 , C12Q2565/501 , C12Q2561/113
摘要： The invention relates a method for detecting and/or quantifying different target molecules being bound at different locations (spots) of a surface of an optically transparent solid support having a refractive index n1 by specific interaction with capture molecules being immobilized on the solid support surface, said solid support being in contact with a solution having refractive index n2, whereby n1 > n2 and wherein the target molecules are in contact with the capture molecules, said method comprising the steps of: a) illuminating homogeneously the surface of the support on which the target molecules are bound, thereby causing homogeneous excitation of the target molecule; b) detecting light emitted from the target molecules in at least four different locations (spots) in the following way: - collecting the emitted light through a side of said support which is inclined relative to the surface of the support on which the target molecules are bound, - focusing the emitted light by a lens on a detector surface which is positioned at an observation angle θobin relative to the normal to the said solid support surface in the support, such that 90° > θobin > sin
-1 (n2/n1), - obtaining an image of at least 4 different locations (spots) being spatially discriminated onto the surface of the detector.

3. 发明授权

EP3183576B1 METHOD FOR COATING A SOLID SUPPORT 有权
公开(公告)号：EP3183576B1
公开(公告)日：2019-11-13
申请号：EP15754181.4
申请日：2015-08-20
申请人： Eppendorf AG
发明人： PLUESTER, Wilhelm , REMACLE, Jose , ALEXANDRE, Isabelle , PLENNEVAUX, Christelle , DE LONGUEVILLE, Francoise
IPC分类号： G01N33/543 , C12M1/32 , C12M1/00

4. 发明公开

EP3183576A1 METHOD FOR COATING A SOLID SUPPORT 审中-公开
标题翻译：涂布固体支持物的方法
公开(公告)号：EP3183576A1
公开(公告)日：2017-06-28
申请号：EP15754181.4
申请日：2015-08-20
申请人： Eppendorf AG
发明人： PLUESTER, Wilhelm , REMACLE, Jose , ALEXANDRE, Isabelle , PLENNEVAUX, Christelle , DE LONGUEVILLE, Francoise
IPC分类号： G01N33/543 , C12M1/32 , C12M1/00
摘要： The present invention relates to a method for coating a solid support which is suitable for being used in cell culture applications. The method comprises a step in which a solution of a carrier protein, which comprises attached thereto or as a part of its amino acid sequence at least one cell function-modulating peptide sequence, is heated to at least 50°C and incubated same under conditions which result in the formation of soluble carrier protein aggregates comprising between 2 and 1000 protein molecules. Subsequently, the protein aggregates are brought into contact with the solid support under conditions that allow for the non-covalent adsorption of the aggregates to the support. The invention also relates to a coated solid support having non-covalently adsorbed on its surface at least one of the above-mentioned aggregate of carrier proteins aggregates.
摘要翻译：本发明涉及涂覆适用于细胞培养应用的固体载体的方法。该方法包括这样一个步骤，其中载体蛋白质的溶液被加热到至少50℃并且在一定条件下温育，所述载体蛋白质的溶液包含与其连接的或作为其氨基酸序列的一部分的至少一种细胞功能调节肽序列其导致形成包含2至1000个蛋白质分子的可溶性载体蛋白质聚集体。随后，使蛋白质聚集体在允许聚集体非共价吸附到载体上的条件下与固体载体接触。本发明还涉及具有非共价吸附在其表面上的至少一种上述载体蛋白质聚集体聚集体的包衣固体载体。

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