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1. 发明公开

EP0085548A3 Cloning vectors 失效
标题翻译：克隆载体
公开(公告)号：EP0085548A3
公开(公告)日：1985-08-21
申请号：EP83300442
申请日：1983-01-28
申请人： ELI LILLY AND COMPANY
发明人： Miller, James Robert , Fayerman, Jeffrey Tobin , Kovacevic, Steven , Malin née Beerman, Nancy Ellen
IPC分类号： C12N15/00
CPC分类号： C12N15/75 , C12N15/76 , Y10S435/832 , Y10S435/849 , Y10S435/886
摘要： The present invention discloses multi-functional recombinant DNA cloning vectors comprising
a) two or more functionally different origins of replication that are indpendently selected from the group consisting of any origin of replication that is functional in Streptomyces, any origin of replication that is functional in Bacillus, and any origin of replication that is functional in E. coli, and b) one or more DNA segments that confer resistance to at least one antibiotic when transformed into a sensitive host cell in which an origin of replication comprising said vector is functional, said host cells being susceptible to transformation, cell division, and culture,
subject to the limitation that when. said vector is limited to two functionally different origins of replication, neither of the origins of replication is functional in E. coli.
摘要翻译：本发明公开了多功能重组DNA克隆载体，其包含a）两种或更多种功能上不同的复制起点，其独立地选自任何在链霉菌中有功能的复制起点，任何在芽孢杆菌中起作用的复制起点，以及在大肠杆菌中有功能的任何复制起点，以及b）一个或多个DNA片段，当转化成其中包含所述载体的复制起点具有功能的敏感宿主细胞时赋予对至少一种抗生素的抗性，所述宿主细胞易受转化，细胞分裂和培养的影响，但受限于何时。所述载体限于两个功能上不同的复制起点，复制起点都不在大肠杆菌中起作用。

2. 发明授权

EP0116411B1 DNA expression vectors useful in bacillus cells 失效
标题翻译： DNA表达载体用于贝类细胞
公开(公告)号：EP0116411B1
公开(公告)日：1990-05-16
申请号：EP84300184.3
申请日：1984-01-12
申请人： ELI LILLY AND COMPANY
发明人： Kovacevic, Steven , Miller, James Robert , Hsiung, Hansen Maxwell
IPC分类号： C12N15/00 , C12P21/02 , C07H21/04 , C12N1/20
CPC分类号： C12N15/76 , C12N15/75

3. 发明授权

EP0117041B1 DNA sequence in streptomyces 失效
标题翻译：链霉菌中的DNA序列
公开(公告)号：EP0117041B1
公开(公告)日：1990-04-25
申请号：EP84300185.0
申请日：1984-01-12
申请人： ELI LILLY AND COMPANY
发明人： Kovacevic, Steven , Fayerman, Jeffrey Tobin , Miller, James Robert , Richardson, Mark Alan
IPC分类号： C12N15/00 , C12N1/20
CPC分类号： C12N15/74 , C12N15/76 , Y10S435/886

4. 发明公开

EP0116411A3 DNA expression vectors useful in bacillus cells 失效
标题翻译： DNA表达载体可用于贝类细胞
公开(公告)号：EP0116411A3
公开(公告)日：1986-03-19
申请号：EP84300184
申请日：1984-01-12
申请人： ELI LILLY AND COMPANY
发明人： Kovacevic, Steven , Miller, James Robert , Hsiung, Hansen Maxwell
IPC分类号： C12N15/00 , C12P21/02 , C07H21/04 , C12N01/20 , C12R01/125 , C12R01/465 , C12R01/19
CPC分类号： C12N15/76 , C12N15/75
摘要： Recombinant DNA expression vectors for use in Bacillus and other host cells are disclosed. The vectors comprise a Bacillus subtilis ribosome binding site-encoding DNA fragment ligated to the veg promoter of B. subtilis and to a gene that encodes a functional polypeptide, the DNA fragment sequence being wherein
A is deoxyadenyl, G is deoxyguanyl, C is deoxycytosyl, T is thymidyl, R is G or C and R' is G or C, subject to the limitation that R and R 1 are not simultaneously the same deoxyribonucleotide.

5. 发明授权

EP0176320B1 A method for expression and secretion in bacillus 失效
标题翻译： BACILLUS中表达和分泌的方法
公开(公告)号：EP0176320B1
公开(公告)日：1990-07-25
申请号：EP85306665.2
申请日：1985-09-19
申请人： ELI LILLY AND COMPANY
发明人： Kovacevic, Steven , Miller, James Robert
IPC分类号： C12N15/00 , C12P21/02 , C12N1/20
CPC分类号： C07K14/62 , C07K2319/00 , C07K2319/02 , C07K2319/036 , C07K2319/75 , C12N9/22 , C12N15/75

6. 发明公开

EP0341892A1 Recombinant DNA expression vectors and DNA compounds that encode deacetoxycephalosporin C synthetase 失效
标题翻译： Rekombinante DNS-Expressionsvektoren und DNS-Zusammensetzungen，diefürDeacetoxycephalosporin C-Synthetase kodieren。
公开(公告)号：EP0341892A1
公开(公告)日：1989-11-15
申请号：EP89304452.9
申请日：1989-05-03
申请人： ELI LILLY AND COMPANY
发明人： Ingolia, Thomas Dominick , Kovacevic, Steven , Miller, James Robert , Skatrud, Paul Luther
IPC分类号： C12N15/00 , C12N1/20 , C12N1/14
CPC分类号： C12N9/0071
摘要： The present invention provides DNA compounds that encode deacetoxycephalosporin C synthetase (DAOCS) activity. The compounds can be used to construct recombinant DNA expression vectors for a wide variety of host cells, including E. coli , Penicillium , Streptomyces , Aspergillus , and Cephalosporium .
摘要翻译：本发明提供编码去乙酰氧基头孢菌素C合成酶（DAOCS）活性的DNA化合物。该化合物可用于构建多种宿主细胞的重组DNA表达载体，包括大肠杆菌，青霉菌，链霉菌属，曲霉属和头孢菌属。

7. 发明公开

EP0116411A2 DNA expression vectors useful in bacillus cells 失效
标题翻译：芽孢杆菌中的ADN-Expressionstoktoren verwendbar。
公开(公告)号：EP0116411A2
公开(公告)日：1984-08-22
申请号：EP84300184.3
申请日：1984-01-12
申请人： ELI LILLY AND COMPANY
发明人： Kovacevic, Steven , Miller, James Robert , Hsiung, Hansen Maxwell
IPC分类号： C12N15/00 , C12P21/02 , C07H21/04 , C12N1/20
CPC分类号： C12N15/76 , C12N15/75
摘要： Recombinant DNA expression vectors for use in Bacillus and other host cells are disclosed. The vectors comprise a Bacillus subtilis ribosome binding site-encoding DNA fragment ligated to the veg promoter of B. subtilis and to a gene that encodes a functional polypeptide, the DNA fragment sequence being wherein

A is deoxyadenyl,
G is deoxyguanyl,
C is deoxycytosyl,
T is thymidyl,
R is G or C and
R' is G or C,
subject to the limitation that R and R 1 are not simultaneously the same deoxyribonucleotide.
摘要翻译：公开了用于芽孢杆菌属和其他宿主细胞的重组DNA表达载体。载体包含与枯草芽孢杆菌的veg启动子连接的枯草芽孢杆菌核糖体结合位点编码DNA片段和编码功能性多肽的基因，DNA片段序列为“IMAGE”，其中A为脱氧腺苷，G为脱氧鸟苷基，C 是脱氧胞嘧啶，T是胸苷酸，R是G或C，R min是G或C，受限于R和R min不同时是脱氧核糖核苷酸。

8. 发明授权

EP0377295B1 Recombinant DNA expression vectors and DNA compounds that encode isopenicillin N epimerase (racemase) activity 失效
标题翻译：含有和异青霉素N差向异构酶（消旋酶）活性编码DNA化合物的重组DNA表达载体。
公开(公告)号：EP0377295B1
公开(公告)日：1995-02-01
申请号：EP89313150.8
申请日：1989-12-15
申请人： ELI LILLY AND COMPANY
发明人： Kovacevic, Steven , Miller, James Robert , Skatrud, Paul Luther , Tobin, Matthew Barry
IPC分类号： C12N15/61 , C12N9/90 , C12N1/21 , C12N1/15
CPC分类号： C12N9/90 , C12N15/80

9. 发明公开

EP0465189A3 Recombinant DNA expression vectors and DNA compounds that encode deacetylcephalosporin C synthase activity 失效
标题翻译：重组DNA表达载体和编码脱乙酰壳多糖C合成酶活性的DNA化合物
公开(公告)号：EP0465189A3
公开(公告)日：1992-03-04
申请号：EP91305939.0
申请日：1991-07-01
申请人： ELI LILLY AND COMPANY
发明人： Kovacevic, Steven , Miller, James Robert
IPC分类号： C12N15/52 , C12N9/00 , C07K15/00 , C12P21/02
CPC分类号： C12N9/0071
摘要： The present invention provides DNA compounds that encode hydroxylase activity of Streptomyces clavuligerus . The compounds can be used to construct recombinant DNA expression vectors for a wide variety of host cells, including E . coli , Penicillium , Streptomyces , Aspergillus , and Cephalosporium .

10. 发明公开

EP0377295A1 Recombinant DNA expression vectors and DNA compounds that encode isopenicillin N epimerase (racemase) activity 失效
标题翻译：含有和异青霉素N差向异构酶（消旋酶）活性编码DNA化合物的重组DNA表达载体。
公开(公告)号：EP0377295A1
公开(公告)日：1990-07-11
申请号：EP89313150.8
申请日：1989-12-15
申请人： ELI LILLY AND COMPANY
发明人： Kovacevic, Steven , Miller, James Robert , Skatrud, Paul Luther , Tobin, Matthew Barry
IPC分类号： C12N15/61 , C12N9/90 , C12N1/21 , C12N1/15
CPC分类号： C12N9/90 , C12N15/80
摘要： The present invention provides DNA compounds that encode isopenicillin N epimerase (racemase) activity. The compounds can be used to construct recombinant DNA expression vectors for a wide variety of host cells, including E. coli , Penicillium , Streptomyces , Aspergillus , and Cephalosporium .
摘要翻译：本发明提供的DNA化合物编码做异青霉素N差向异构酶（消旋酶）活性。该化合物可用于构建重组DNA表达载体为多种宿主细胞，包括大肠杆菌（E.coli），青霉属，链霉菌属，曲霉属，和头孢的。

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