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标题翻译： XYLANASEN ZUR SOLUBILISIERUNG VON阿拉伯酚醛树脂材料

公开(公告)号：EP2880157A1

公开(公告)日：2015-06-10

申请号：EP13744573.0

申请日：2013-08-02

申请人： Dupont Nutrition Biosciences ApS

发明人： ARENT, Susan Lund , LAURSEN, Brian Søgaard , KIARIE, Elijah Gituanjah , MILLÁN, Luis Fernando Romero , ZHANG, Zhenghong , LAU, Rosalyn , YU, Zheyong , KOOY, Floor Klaaske , VAN TUIJL, Jan Hendrik , KOOPS, Bart Christiaan , PEDERSEN, Mads Brøgger , DALSGAARD, Søren

IPC分类号： C12N9/24 , C12P7/08

CPC分类号： C12N9/2482 , A23K10/10 , A23K10/14 , A23K10/38 , A23K50/30 , A23K50/75 , C12C5/004 , C12P5/007 , C12P7/10 , C12P19/14 , C12Y302/01008 , C13K1/02 , C13K1/06 , Y02E50/16 , Y02E50/17 , Y02P60/873

摘要： The present invention relates to a method for solubilising arabinoxylan-containing material (particularly insoluble arabinoxylan-containing material), comprising admixing a xylan-containing material with a xylanase comprising a polypeptide sequence shown herein as SEQ ID No. 3, SEQ ID No. 2, SEQ ID No. 1, SEQ ID No. 9, SEQ ID No. 10, SEQ ID No. 11 or SEQ ID No. 15, or a variant, homologue, fragment or derivative thereof having at least 75% identity with SEQ ID No. 3 or SEQ ID No. 2 or SEQ ID No. 1 or SEQ ID No. 9 or SEQ ID No. 10 or SEQ ID No. 11 or SEQ ID No. 15; or a polypeptide sequence which comprises SEQ ID No. 3, SEQ ID No. 2, SEQ ID No. 1, SEQ ID No. 9, SEQ ID No. 10, SEQ ID No. 11 or SEQ ID No. 15 with a conservative substitution of at least one of the amino acids; or a xylanase which is encoded by a nucleotide sequence shown herein as SEQ ID No. 6, SEQ ID No. 5, SEQ ID No. 4, SEQ ID No. 12, SEQ ID No. 13, SEQ ID No. 14, SEQ ID No. 16, SEQ ID No. 17 or SEQ ID No. 18, or a nucleotide sequence which can hybridize to SEQ ID No. 6, SEQ ID No. 5, SEQ ID No. 4, SEQ ID No. 12, SEQ ID No. 13, SEQ ID No. 14, SEQ ID No. 16, SEQ ID No. 17 or SEQ ID No. 18 under high stringency conditions, or a nucleotide sequence which has at least 75% identity with SEQ ID No. 6, SEQ ID No. 5, SEQ ID No. 4, SEQ ID No. 12, SEQ ID No. 13, SEQ ID No. 14, SEQ ID No. 16, SEQ ID No. 17 or SEQ ID No. 18, or a nucleotide sequence which differs from SEQ ID No. 6 or SEQ ID No. 5 or SEQ ID No. 4 or SEQ ID No. 12 or SEQ ID No. 13 or SEQ ID No. 14 or SEQ ID No. 16 or SEQ ID No. 17 or SEQ ID No. 18 due to the degeneracy of the genetic code, or a xylanase obtainable (or obtained) from Fusarium verticilloides. The present invention also relates to a novel xylanase comprising (or consisting of) a polypeptide sequence shown herein as SEQ ID No. 3, SEQ ID No. 2 or SEQ ID No. 1, or a variant, homologue, fragment or derivative thereof having at least 99% identity with SEQ ID No. 3 or SEQ ID No. 2 or SEQ ID No. 1; or a xylanase which is encoded by a nucleotide sequence shown herein as SEQ ID No. 6, SEQ ID No. 5 or SEQ ID No. 4, or a nucleotide sequence which can hybridize to SEQ ID No. 4 or SEQ ID No. 5 under high stringency conditions, or a nucleotide sequence which has at least 97.7% identity (preferably 98% identity) with SEQ ID No. 6, SEQ ID No. 5 or SEQ ID No. 4. The present invention yet further relates to methods relating to feedstuffs, malting and brewing, processing of grain-based materials such as during the production of bioethanol or biochemical (e.g. bio-based isopropanol), or wheat gluten-starch separation processes and the like.

摘要翻译：本发明涉及一种用于增溶含阿拉伯木聚糖的材料（特别是不溶性阿拉伯木聚糖材料）的方法，包括将含木聚糖的材料与包含如SEQ ID No.3，SEQ ID No.2所示的多肽序列的木聚糖酶混合，SEQ ID No.1，SEQ ID No.9，SEQ ID No.10，SEQ ID No.11或SEQ ID No.15的变体，同源物，片段或衍生物，其与SEQ ID No： 3或SEQ ID No.2或SEQ ID No.1或SEQ ID No.9或SEQ ID No.10或SEQ ID No.11或SEQ ID No.15; 或包含SEQ ID No.3，SEQ ID No.2，SEQ ID No.1，SEQ ID No.9，SEQ ID No.10，SEQ ID No.11或SEQ ID No.15的多肽序列与保守的取代至少一个氨基酸; 或由本文所示的核苷酸序列编码的木聚糖酶，如SEQ ID No.6，SEQ ID No.5，SEQ ID No.4，SEQ ID No.12，SEQ ID No.13，SEQ ID NO.14，SEQ 可以与SEQ ID No.6，SEQ ID No.5，SEQ ID No.4，SEQ ID No.12，SEQ ID No.6，SEQ ID No. ID号13，SEQ ID No.14，SEQ ID No.16，SEQ ID No.17或SEQ ID No.18，或与SEQ ID No.6具有至少75％同一性的核苷酸序列，SEQ ID No.5，SEQ ID No.4，SEQ ID No.12，SEQ ID No.13，SEQ ID No.14，SEQ ID No.16，SEQ ID No.17或SEQ ID No.18，或不同于SEQ ID No.6或SEQ ID No.5或SEQ ID No.4或SEQ ID No.12或SEQ ID No.13或SEQ ID No.14或SEQ ID No.16或SEQ ID NO.1的核苷酸序列由于遗传密码的简并性，或者从镰刀菌（Fusarium verticilloides）获得的（或获得的）木聚糖酶，可以得到17号或SEQ ID No.18。本发明还涉及包含（或由其组成）的本文作为SEQ ID No.3，SEQ ID No.2或SEQ ID No.1的多肽序列的新型木聚糖酶，或其变体，同系物，片段或衍生物，其具有与SEQ ID No.3或SEQ ID No.2或SEQ ID No.1的至少99％同一性; 或由本文所示的核苷酸序列编码的木聚糖酶，如SEQ ID No.6，SEQ ID No.5或SEQ ID No.4，或可以与SEQ ID No.4或SEQ ID No.5杂交的核苷酸序列或高度严格条件下的核苷酸序列，或与SEQ ID No.6，SEQ ID No.5或SEQ ID No.4具有至少97.7％同一性（优选98％同一性）的核苷酸序列。本发明还涉及与饲料，麦汁和酿造，生产乙醇或生化（例如生物基异丙醇）期间的谷物材料的加工，或小麦谷蛋白 - 淀粉分离方法等。

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