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1. 发明公开

EP2560475A4 COMBINATIONS INCLUDING CRY34AB/35AB AND CRY3AA PROTEINS TO PREVENT DEVELOPMENT OF RESISTANCE IN CORN ROOTWORMS (DIABROTICA SPP.) 有权
公开(公告)号：EP2560475A4
公开(公告)日：2013-10-23
申请号：EP11772785
申请日：2011-04-22
申请人： DOW AGROSCIENCES LLC
发明人： NARVA KENNETH E , MEADE THOMAS , FENCIL KRISTIN J , LI HUARONG , HEY TIMOTHY D , WOOSLEY AARON T , OLSON MONICA BRITT
IPC分类号： A01H5/00 , A01H5/10 , A01N63/02 , C12N5/04 , C12N15/82
CPC分类号： C12N15/8286 , A01G1/001 , A01H5/10 , A01N37/18 , A01N43/50 , B32B1/02 , B65D85/00 , C07K14/325 , Y02A40/162 , Y10T428/13
摘要： The subject invention relates in part to Cry34Ab/35Ab in combination with Cry6Aa. The subject invention relates in part to the surprising discovery that combinations of Cry34Ab/Cry35Ab and Cry6Aa are useful for preventing development of resistance (to either insecticidal protein system alone) by a corn rootworm (Diabrotica spp.) population. Included within the subject invention are plants producing these insecticidal Cry proteins, which are useful to mitigate concern that a corn rootworm population could develop that would be resistant to either of these insecticidal protein systems alone. Plants (and acreage planted with such plants) that produce these two insecticidal protein systems are included within the scope of the subject invention. The subject invention also relates in part to combinations of Cry34Ab/35Ab and Cry3Aa proteins "triple stacked" with a Cry6Aa protein. Transgenic plants, including corn, comprising a cry6Aa gene, cry34Ab/35Ab genes, and a cry3Aa gene are included within the scope of the subject invention. Thus, such embodiments target rootworms with three modes of action.

2. 发明公开

EP2739133A4 USE OF DIG3 INSECTICIDAL CRYSTAL PROTEIN IN COMBINATION WITH CRY1AB 审中-公开
标题翻译：杀虫DIG3晶体蛋白转cry1Ab中的联合应用
公开(公告)号：EP2739133A4
公开(公告)日：2014-12-17
申请号：EP12822625
申请日：2012-08-03
申请人： DOW AGROSCIENCES LLC
发明人： BURTON STEPHANIE L , MEADE THOMAS , NARVA KENNETH , SHEETS JOEL J , STORER NICHOLAS P , WOOSLEY AARON T
IPC分类号： A01H5/00 , C12N15/82
CPC分类号： C12N15/8286 , Y02A40/162
摘要： The subject invention includes methods and plants for controlling European corn borer, said plants comprising a Cry1Ab insecticidal protein and a DIG-3 insecticidal protein to delay or prevent development of resistance by the insect.

3. 发明公开

EP2560476A4 COMBINATIONS INCLUDING CRY34AB/35AB AND CRY3BA PROTEINS TO PREVENT DEVELOPMENT OF RESISTANCE IN CORN ROOTWORMS (DIABROTICA SPP.) 有权
标题翻译： WITH CRY34AB / 35AB- AND的Cry3Aa蛋白结合，阻止抗性的玉米根钻DEVELOPMENT（叶甲属）。
公开(公告)号：EP2560476A4
公开(公告)日：2013-10-23
申请号：EP11772786
申请日：2011-04-22
申请人： DOW AGROSCIENCES LLC
发明人： NARVA KENNETH E , MEADE THOMAS , FENCIL KRISTIN J , LI HUARONG , HEY TIMOTHY D , WOOSLEY AARON T , OLSON MONICA BRITT
IPC分类号： A01H5/00 , A01H5/10 , A01N63/02 , C12N5/04 , C12N15/82
CPC分类号： C12N15/8286 , A01G1/001 , A01H5/10 , A01N37/18 , A01N43/50 , B32B1/02 , B65D85/00 , C07K14/325 , Y02A40/162 , Y10T428/13
摘要： The subject invention relates in part to Cry34Ab/35Ab in combination with Cry6Aa. The subject invention relates in part to the surprising discovery that combinations of Cry34Ab/Cry35Ab and Cry6Aa are useful for preventing development of resistance (to either insecticidal protein system alone) by a corn rootworm (Diabrotica spp.) population. Included within the subject invention are plants producing these insecticidal Cry proteins, which are useful to mitigate concern that a corn rootworm population could develop that would be resistant to either of these insecticidal protein systems alone. Plants (and acreage planted with such plants) that produce these two insecticidal protein systems are included within the scope of the subject invention. The subject invention also relates in part to combinations of Cry34Ab/35Ab and Cry3Aa proteins "triple stacked" with a Cry6Aa protein. Transgenic plants, including corn, comprising a cry6Aa gene, cry34Ab/35Ab genes, and a cry3Aa gene are included within the scope of the subject invention. Thus, such embodiments target rootworms with three modes of action.

4. 发明公开

EP2512223A4 INSECTICIDAL PROTEIN COMBINATION COMPRISING CRY1AB AND CRY2AA FOR CONTROLLING EUROPEAN CORN BORER, AND METHODS FOR INSECT RESISTANCE MANAGEMENT 有权
标题翻译：转cry1Ab和Cry2Aa杀虫剂蛋白结合，打击以INSECTICIDE管理玉米螟和方法
公开(公告)号：EP2512223A4
公开(公告)日：2013-10-02
申请号：EP10838262
申请日：2010-12-16
申请人： DOW AGROSCIENCES LLC
发明人： MEADE THOMAS , NARVA KENNETH , STORER NICHOLAS P , SHEETS JOEL J , WOOSLEY AARON T , BURTON STEPHANIE L
IPC分类号： C12N15/82 , A01H5/10
CPC分类号： C12N15/8286 , A01N63/02 , Y02A40/162 , A01N65/00 , A01N2300/00
摘要： The subject invention relates in part to stacking certain Cry genes along with Cry 1Fa to result in products that are more durable and less prone towards insects developing resistance towards the activity of any of the toxins (such as Cry 1Fa) by itself. Embodiments of the Cry 1F stacking partners include Cry2Aa, Cry 1I, and Cry 1E. These stacks can be used to control FAW and/or ECB as described herein.
摘要翻译：本主题发明部分地涉及堆叠某些基因的Cry惊魂地连同1FA导致产品确实是更耐用并且朝向昆虫发展朝向任何的毒素的活性的电阻不易发生（叫声：如1FA）本身。的惊魂1F堆叠伙伴实施方案包括Cry2Aa，惊魂1I中，与哭1E。这些堆叠可以用作在描述控制FAW和/或ECB。

5. 发明公开

EP3240560A4 MODIFIED Cry1Ca TOXINS USEFUL FOR CONTROL OF INSECT PESTS 有权转让
公开(公告)号：EP3240560A4
公开(公告)日：2018-05-30
申请号：EP15875965
申请日：2015-12-16
申请人： DOW AGROSCIENCES LLC
发明人： SHEETS JOEL J , NARVA KENNETH , MEADE THOMAS , HEY TIMOTHY D , TAN SEK YEE , ETTER AUDREY JANE , GLANCY TODD P , ARMSTRONG JANNA MAI , CORAM TRISTAN E , MADDURI KRISHNA M , KING JAMES E , LEE RYAN M , LIN GAOFENG , LI JIANQUAN
IPC分类号： A61K38/00 , A01N63/02 , A61K38/16 , C07K1/00 , C07K14/00 , C07K17/00 , C12N15/82
CPC分类号： C07K14/325 , A01N37/46 , A01N63/02 , C12N15/8286 , Y02A40/162
摘要： The subject invention concerns Bacillus thuringiensis modified Cry1Ca insecticidal toxins and the polynucleotide sequences which encode these toxins. Uses in transgenic plants are described as are methods for protecting crops from insect pest damage.

6. 发明公开

EP2560478A4 COMBINATIONS INCLUDING CRY3AA AND CRY6AA PROTEINS TO PREVENT DEVELOPMENT OF RESISTANCE IN CORN ROOTWORMS (DIABROTICA SPP.) 有权
标题翻译： KOMBINATIONEN MIT CRY3AA- UND CRY6AA-PROTEINEN ZUR VERHINDERUNG DER ENTWICKLUNG EINES WIDERSTANDS IN MAISWURZELBOHRERN（DIABROTICA SPP。）
公开(公告)号：EP2560478A4
公开(公告)日：2013-12-04
申请号：EP11772790
申请日：2011-04-22
申请人： DOW AGROSCIENCES LLC
发明人： NARVA KENNETH E , MEADE THOMAS , FENCIL KRISTIN J , LI HUARONG , HEY TIMOTHY D , WOOSLEY AARON T , OLSON MONICA BRITT
IPC分类号： A01H5/00 , A01H5/10 , A01N63/02 , C12N5/04 , C12N15/82
CPC分类号： C12N15/8286 , A01G1/001 , A01H5/10 , A01N37/18 , A01N43/50 , B32B1/02 , B65D85/00 , C07K14/325 , Y02A40/162 , Y10T428/13
摘要： The subject invention relates in part to Cry34Ab/35Ab in combination with Cry6Aa. The subject invention relates in part to the surprising discovery that combinations of Cry34Ab/Cry35Ab and Cry6Aa are useful for preventing development of resistance (to either insecticidal protein system alone) by a corn rootworm (Diabrotica spp.) population. Included within the subject invention are plants producing these insecticidal Cry proteins, which are useful to mitigate concern that a corn rootworm population could develop that would be resistant to either of these insecticidal protein systems alone. Plants (and acreage planted with such plants) that produce these two insecticidal protein systems are included within the scope of the subject invention. The subject invention also relates in part to combinations of Cry34Ab/35Ab and Cry3Aa proteins "triple stacked" with a Cry6Aa protein. Transgenic plants, including corn, comprising a cry6Aa gene, cry34Ab/35Ab genes, and a cry3Aa gene are included within the scope of the subject invention. Thus, such embodiments target rootworms with three modes of action.
摘要翻译：本发明部分涉及与Cry6Aa组合的Cry34Ab / 35Ab。本发明部分涉及令人惊讶的发现，即Cry34Ab / Cry35Ab和Cry6Aa的组合可用于预防玉米根虫（Diabrotica spp。）种群对抗（单独针对杀虫蛋白系统）的抗性发展。包括在本发明中的是产生这些杀虫Cry蛋白的植物，其可用于减轻玉米根虫群体可能发展的对单独对这些杀虫蛋白系统中的任一个有抗性的担忧。产生这两种杀虫蛋白质系统的植物（和种植这些植物的种植面积）包括在本发明的范围内。本发明还部分涉及Cry3Ab / 35Ab和Cry3Aa蛋白与Cry6Aa蛋白“三重堆叠”的组合。包含cry6Aa基因，cry34Ab / 35Ab基因和cry3Aa基因的转基因植物，包括玉米，包括在本发明的范围内。因此，这些实施方案以三种作用模式针对根虫。

7. 发明公开

EP2513318A4 USE OF CRY1AB IN COMBINATION WITH CRY1BE FOR MANAGEMENT OF RESISTANT INSECTS 有权
标题翻译： VERWENDUNG VON CRY1AB在KOMBINATION MIT CRY1BE ZURBEKÄMPFUNGRESISTENTER INSEKTEN
公开(公告)号：EP2513318A4
公开(公告)日：2013-10-02
申请号：EP10842620
申请日：2010-12-16
申请人： DOW AGROSCIENCES LLC
发明人： MEADE THOMAS , NARVA KENNETH , STORER NICHOLAS P , SHEETS JOEL J , WOOSLEY AARON T , BURTON STEPHANIE L
IPC分类号： C12N15/82 , A01H5/00 , A01H5/10 , A01N63/02 , A01N65/00
CPC分类号： C12N15/8286 , A01N63/02 , A01N65/00 , Y02A40/162 , A01N2300/00
摘要： The subject invention relates in part to stacking Cry1Be toxins along with Cry1Fa toxins to prevent insects from developing resistance towards either toxin by itself. As discussed in more detail herein, the subject pair of proteins is a particularly advantageous combination, as no other pair of proteins is known to provide high levels of control and non-cross-resistant activity against both Spodoptera frugiperda (FAW) and Ostrinia nubilalis (ECB) insects. This dual, non-cross-resistant activity is also advantageous because it can reduce the number of proteins/genes needed to target these insects with multiple, non-cross-resistant proteins. This can reduce or eliminate the need for refuge acreage. Accordingly, the subject invention also relates generally to using four genes to provide three proteins for non-cross-resistant control of a first insect, and three proteins for non-cross-resistant control of a second insect. In preferred embodiments, the targeted insects are FAW and ECB.
摘要翻译：本发明部分地涉及将Cry1Be毒素与Cry1Fa毒素一起堆叠以防止昆虫本身对毒素产生抗性。如本文更详细讨论的那样，本发明的一对蛋白质是特别有利的组合，因为没有其他蛋白质对可以提供针对草地贪夜蛾（One Spodoptera frugiperda，FAW）和鸵鸟（Ostrinia nubilalis）的高水平控制和非交叉抗性活性 ECB）昆虫。这种双重非交叉抗性活性也是有利的，因为它可以减少用多种非交叉抗性蛋白质靶向这些昆虫所需的蛋白质/基因的数量。这可以减少或消除避难所面积的需要。因此，本发明还通常涉及使用四个基因提供三种用于第一种昆虫的非交叉耐药性控制的蛋白质和三种用于非交叉耐药性控制第二种昆虫的蛋白质。在优选的实施方案中，目标昆虫是FAW和ECB。

8. 发明公开

EP2513315A4 COMBINED USE OF CRY1FA AND CRY1AB PROTEINS FOR CONTROL OF CRY-RESISTANT SUGARCANE BORER AND FOR INSECT RESISTANCE MANAGEMENT IN SUGARCANE 审中-公开
标题翻译：作者CRY1FA-和Cry1Ab蛋白组合使用，进行战斗CRY性甘蔗演习和杀虫剂管理甘蔗
公开(公告)号：EP2513315A4
公开(公告)日：2013-08-21
申请号：EP10842616
申请日：2010-12-16
申请人： DOW AGROSCIENCES LLC
发明人： MEADE THOMAS , NARVA KENNETH , STORER NICHOLAS P , SHEETS JOEL J , BURTON STEPHANIE L
IPC分类号： C12N15/82 , A01H5/04
CPC分类号： C12N15/8286 , Y02A40/162
摘要： The subject invention includes methods and sugarcane plants for controlling sugarcane borer (SCB) insects, said sugarcane plants comprising Cry1Fa and Cry1Ab core toxin containing proteins in combination to delay or prevent development of resistance by the SCB.

9. 发明公开

EP2512220A4 INSECTICIDAL PROTEIN COMBINATIONS FOR CONTROLLING FALL ARMYWORM AND EUROPEAN CORN BORER, AND METHODS FOR INSECT RESISTANCE MANAGEMENTS 有权
标题翻译：杀虫剂蛋白结合，打击以INSECTICIDE管理行军虫和欧洲玉米螟，和方法
公开(公告)号：EP2512220A4
公开(公告)日：2013-09-25
申请号：EP10838258
申请日：2010-12-16
申请人： DOW AGROSCIENCES LLC
发明人： MEADE THOMAS , NARVA KENNETH , STORER NICHOLAS P , SHEETS JOEL J , BURTON STEPHANIE L
IPC分类号： C12N15/82 , A01H5/00 , A01H5/10 , A01N63/02 , A01N65/00
CPC分类号： C12N15/8286 , A01N63/02 , Y02A40/162 , A01N65/00 , A01N2300/00
摘要： The subject invention relates in part to stacking certain Cry genes along with Cry 1Fa to result in products that are more durable and less prone towards insects developing resistance towards the activity of any of the toxins (such as Cry 1Fa) by itself. Embodiments of the Cry 1F stacking partners include Cry2Aa, Cry 1I, and Cry 1E. These stacks can be used to control FAW and/or ECB as described herein.
摘要翻译：本主题发明部分地涉及堆叠某些基因的Cry惊魂地连同1FA导致产品确实是更耐用并且朝向昆虫发展朝向任何的毒素的活性的电阻不易发生（叫声：如1FA）本身。的惊魂1F堆叠伙伴实施方案包括Cry2Aa，惊魂1I中，与哭1E。这些堆叠可以用作在描述控制FAW和/或ECB。

10. 发明公开

EP2512218A4 INSECT RESISTANCE MANAGEMENT WITH COMBINATIONS OF CRY1BE AND CRY1F PROTEINS 有权
标题翻译： INSEKTENBEKÄMPFUNGSMANAGEMENTMIT KOMBINATIONEN AUS CRY1BE- UND CRY1F-PROTEINEN
公开(公告)号：EP2512218A4
公开(公告)日：2013-09-25
申请号：EP10838256
申请日：2010-12-16
申请人： DOW AGROSCIENCES LLC
发明人： MEADE THOMAS , NARVA KENNETH , STORER NICHOLAS P , SHEETS JOEL J , WOOSLEY AARON T , BURTON STEPHENIE L
IPC分类号： C12N15/82 , A01H5/00 , A01H5/10 , A01N63/02 , A01N65/00
CPC分类号： C12N15/8286 , A01N63/02 , A01N65/00 , Y02A40/162 , A01N2300/00
摘要： The subject invention relates in part to stacking Cry1Be toxins along with Cry1Fa toxins to prevent insects from developing resistance towards either toxin by itself. As discussed in more detail herein, the subject pair of proteins is a particularly advantageous combination, as no other pair of proteins is known to provide high levels of control and non-cross-resistant activity against both Spodoptera frugiperda (FAW) and Ostrinia nubilalis (ECB) insects. This dual, non-cross-resistant activity is also advantageous because it can reduce the number of proteins/genes needed to target these insects with multiple, non-cross-resistant proteins. This can reduce or eliminate the need for refuge acreage. Accordingly, the subject invention also relates generally to using four genes to provide three proteins for non-cross-resistant control of a first insect, and three proteins for non-cross-resistant control of a second insect. In preferred embodiments, the targeted insects are FAW and ECB.
摘要翻译：本发明部分地涉及将Cry1Be毒素与Cry1Fa毒素一起堆叠以防止昆虫本身对毒素产生抗性。如本文更详细讨论的那样，本发明的一对蛋白质是特别有利的组合，因为没有其他蛋白质对可以提供针对草地贪夜蛾（One Spodoptera frugiperda，FAW）和鸵鸟（Ostrinia nubilalis）的高水平的控制和非交叉抗性活性 ECB）昆虫。这种双重非交叉抗性活性也是有利的，因为它可以减少用多种非交叉抗性蛋白质靶向这些昆虫所需的蛋白质/基因的数量。这可以减少或消除避难所面积的需要。因此，本发明还通常涉及使用四个基因来提供用于第一昆虫的非交叉耐药性控制的三种蛋白质和用于第二种昆虫的非交叉耐药性控制的三种蛋白质。在优选的实施方案中，目标昆虫是FAW和ECB。

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