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    • 6. 发明公开
    • ARTIFIZIELLE FORISOMENKÖRPER MIT SEO-F-FUSIONSPROTEINEN, PFLANZLICHE ODER HEFEZELLEN MIT VEKTOREN, DIE FÜR DIESE PROTEINE CODIEREN, SOWIE VEKTOREN, DIE FÜR SEO-F-FUSIONSPROTEINE CODIEREN
    • 与这种蛋白质编码载体和载体的搜索引擎优化F-融合蛋白编码SEO F-FUSION蛋白,植物或酵母细胞人工FORISOMENKÖRPER
    • EP2847334A1
    • 2015-03-18
    • EP13723041.3
    • 2013-05-02
    • Fraunhofer Gesellschaft zur Förderung der angewandten Forschung e.V.
    • MÜLLER, BojePRÜFER, DirkFISCHER, Rainer
    • C12N15/29C12N5/10C12N15/81C12N15/62C12N11/00C07K14/415
    • The invention relates to an artificial forisome body which comprises a fusion protein consisting of at least one SEO-F protein or a section thereof that comprises at least 50 amino acids, and at least one additional protein or peptide with the exception of the GFP and Venus proteins, in which (a) said additional protein or peptide has a mass of no more than 30 kDa, and/or (b) the forisome body also comprises a non-fusion SEO-F protein or a form of this protein that is C-terminally deleted by up to 45 amino acids and/or N-terminally deleted by up to 13 amino acids, the non-fusion SEO-F protein being selected from those proteins which have the property of being able to form homomeric forisome bodies in the absence of additional SEO-F proteins, or (c) the additional protein or peptide is a section of a second SEO-F protein provided that one of the two SEO-F proteins, in a non-fusion form, is capable of forming homomeric forisome bodies, and the fusion protein consists of an N-terminal SEO-F protein section and a C-terminal SEO-F protein section, said sections constituting an SEO-F protein that is complete or C-terminally deleted by up to 50 amino acids and/or N-terminally deleted by up to 13 amino acids, provided that, if required, any number of the four cysteines present in the native SEO-F proteins in the C-terminal section between AS 600 and AS 670 may be exchanged for amino acids that are unable to form an S-S bridge bond. The invention also relates to a plant cell or yeast cell comprising a vector that codes for the given fusion protein and, if required, also for the non-fusion SEO-F protein or its deleted form as defined in (b), as well as to a vector that can be multiplied in a yeast cell and that comprises a section which codes for the given fusion protein. The artificial forisome body of the invention is suitable for protein-chemical purposes, in particular for providing immobilised enzymes, antibodies, antigens and such proteins or peptides that can be immobilised, on a substrate, on the basis of an affinity reaction with a substrate-bound biological or biochemically-produced material.
    • 人工forisome体包括(a)由forisomes(SEO-F)蛋白或该蛋白质的形式的筛元件闭塞所做的是C末端至多45个氨基酸和/或N-末端至多13个氨基酸缺失缺失 ,其中SEO-F蛋白具有能够形成同聚在没有附加SEO-F蛋白的forisome体的性质,和(b)的融合蛋白由至少一种SEO-F蛋白的或SEO的截面 F蛋白确实包含至少50种氨基酸和至少一种另外的蛋白质或肽。 人工forisome体包括(a)由forisomes(SEO-F)蛋白或该蛋白质的形式的筛元件闭塞所做的是C末端至多45个氨基酸和/或N-末端至多13个氨基酸缺失缺失 ,其中SEO-F蛋白具有能够形成同聚在没有附加SEO-F蛋白的forisome体的性质,和(b)的融合蛋白由至少一种SEO-F蛋白的或SEO的截面 F蛋白确实包含至少50个氨基酸和至少一种另外的蛋白质或肽由......组成生物化学活性或可激活蛋白质或肽的,条件是,如果需要的话,任何数量存在于C.天然SEO-F蛋白的四个半胱氨酸的 AS 600和AS之间的670末端部的更换氨基酸确实是不能形成在SS桥键。 独立权利要求中包括了:(1)植物细胞或酵母细胞,其包含(a)第一向量做为SEO-F蛋白的代码,以及(b)第二向量做融合蛋白码; (2)在酵母细胞,其包含的部分再现的向量做融合蛋白码; 和(3)使用融合蛋白,包括在SEO-F蛋白和另外的蛋白质或肽,用于人造forisome体的制备。