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2. 发明公开

EP1564285A1 METHOD OF OBTAINING CELL LINES IN A PROTEIN-FREE MEDIUM AND CELL LINES THUS OBTAINED 有权
标题翻译：程序用于获得适于生长无血清和蛋白MEDIA重组哺乳动物骨髓瘤细胞系
公开(公告)号：EP1564285A1
公开(公告)日：2005-08-17
申请号：EP03757654.3
申请日：2003-10-22
申请人： Centro de Inmunologia Molecular
发明人： PEREZ RODRIGUEZ, Rolando , CASTILLO VITLLOCH, Adolfo , VITORES SARAZOLA, Svieta , BOGGIANO AYO, Tammy , ROJAS DEL CALVO, Luis
IPC分类号： C12N5/00 , C12P21/08
CPC分类号： C07K16/2809 , C07K16/2863 , C07K16/2896 , C07K2317/24 , C12N5/0693 , C12N5/0694 , C12N2500/95 , C12N2510/02
摘要： The present invention relates to a method of recovering mammalian cell clones adapted to serum and protein-free media, the procedure includes a two-stage adaptation process to grow in that condition. The present invention discloses a critical protein concentration interval in which cells must grow in order to gain the capacity to survive in serum and protein-free condition, once the cells have grown at the critical interval concentrations, subsequent decreases of the concentration will affect neither viability nor cellular doubling time. The critical protein concentration interval is cell line specific.
Furthermore, in the present invention mammalian cells clones are disclosed, which are stable in serum- and protein-free media for at least 40 generations; additionally, clones disclosed in the present invention express a recombinant product.
The cell clones disclosed in the present invention produce the humanized anti-EGF-R antibody hR3, the humanized anti-CD6 antibody T1hT, the chimeric anti CD3 antibody T3Q, or fragments thereof.
摘要翻译：本发明涉及回收哺乳动物细胞克隆angepasst血清和无蛋白质培养基，该过程包括两个阶段的适应过程在条件生长做的方法。本发明盘松动，其中细胞必须按顺序生长获得的能力，血清和无蛋白质条件生存，一旦细胞已经在临界间隔浓度生长的临界蛋白浓度间隔中，浓度的后续的减量，将既不影响生存力也不蜂窝倍增时间。临界蛋白质浓度间隔是细胞系特异的。进一步地，在本发明的哺乳动物细胞克隆是游离缺失盘，这是在无血清和无蛋白质培养基为至少40个世代稳定; 另外，在本发明的克隆游离缺失盘表达的重组产物。在本发明中的游离缺失细胞克隆盘产生的人源化抗EGF-R抗体HR3，人源化抗CD6抗体T1hT，嵌合抗CD3抗体T3Q，或其片段。

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