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1. 发明授权

EP1981914B1 CONSTRUCTION OF NEW VARIANTS OF DEXTRANSUCRASE DSR-S BY GENETIC ENGINEERING 有权
标题翻译：基因工程技术构建DEXTRANSUCRASE DSR-S新变异体
公开(公告)号：EP1981914B1
公开(公告)日：2016-05-11
申请号：EP07734270.7
申请日：2007-02-08
申请人： Centre National de la Recherche Scientifique , Institut National de Recherche Agronomique , Institut National des Sciences Appliquées de Toulouse
发明人： MONSAN, Pierre , REMAUD-SIMEON, Magali , POTOCKI-VERONESE, Gabrielle , MOULIS, Claire
IPC分类号： C07K19/00 , C12N15/54 , C12N9/10 , C12P19/04 , C12P19/18
CPC分类号： C12N9/1051 , C07H3/06 , C07K2319/35 , C08B37/0021 , C12P19/04 , C12P19/08 , C12P19/12 , C12P19/18 , C12Y204/01005 , Y02P20/52
摘要： The present invention relates to a recombinant process for the production of truncated and/or mutated dextransucrases while conserving their enzymatic activity and/or their specificity in the synthesis of the ±-1,6 bonds. More precisely, the present invention relates to nucleic acid sequences of truncated and/or mutated dextransucrases, vectors containing said nucleic acid sequences and host cells transformed by sequences encoding truncated and/or mutated dextransucrases. In a further aspect, the invention concerns a method for producing, in a recombinant manner, truncated and/or mutated dextransucrases which conserve their enzymatic activity and/or which conserve their specificity in the synthsis of ±-1,6 bonds and however can produce, from saccharose, dextrans with high molar mass and with modified rheological properties, compared with the properties of dextran obtained with the native enzyme in the same conditions and isomalto-oligosaccharides with a controlled molar mass and dextrans. The dextrans and IMO of the invention can be used namely as texturing agents or as prebiotics.

3. 发明公开

EP3103874A1 CONSTRUCTION OF NEW VARIANTS OF DEXTRANSUCRASE DSR-S BY GENETIC ENGINEERING 审中-公开
标题翻译： KONSTRUKTION中性粒细胞转录酶DSR-S MITTELS GENTECHNOLOGIE
公开(公告)号：EP3103874A1
公开(公告)日：2016-12-14
申请号：EP16162913.4
申请日：2007-02-08
申请人： Centre National de la Recherche Scientifique , Institut National de la Recherche Agronomique , Institut National des Sciences Appliquées de Toulouse
发明人： MONSAN, Pierre , REMAUD-SIMEON, Magali , POTOCKI-VERONESE, Gabrielle , MOULIS, Claire
IPC分类号： C12N15/54 , C12N9/10 , C12P19/04 , C12P19/18 , C07K19/00
摘要： The present invention relates to a recombinant process for the production of truncated and/or mutated dextransucrases while conserving their enzymatic activity and/or their specificity in the synthesis of the alpha-1,6 bonds. More precisely, the present invention relates to nucleic acid sequences of truncated and/or mutated dextransucrases, vectors containing said nucleic acid sequences and host cells transformed by sequences encoding truncated and/or mutated dextransucrases. In a further aspect, the invention concerns a method for producing, in a recombinant manner, truncated and/or mutated dextransucrases which conserve their enzymatic activity and/or which conserve their specificity in the synthesis of alpha-1,6 bonds and however can produce, from saccharose, dextrans with high molar mass and with modified rheological properties, compared with the properties of dextran obtained with the native enzyme in the same conditions and isomalto-oligosaccharides with a controlled molar mass and dextrans. The dextrans and IMO of the invention can be used namely as texturing agents or as prebiotics.
摘要翻译：本发明涉及用于在保护其酶活性和/或其在α-1,6键合成中的特异性的同时产生截短和/或突变的葡聚糖蔗糖酶的重组方法。更准确地说，本发明涉及截短和/或突变的葡聚糖蔗糖酶的核酸序列，含有所述核酸序列的载体和通过编码截短和/或突变的葡聚糖蔗糖酶的序列转化的宿主细胞。在另一方面，本发明涉及一种以重组方式生产截短和/或突变的葡聚糖蔗糖酶的方法，其保留其酶活性和/或保护其在α-1,6键合成中的特异性，然而可产生与具有高摩尔质量和具有改进的流变性质的蔗糖，葡聚糖相比，在相同条件下与天然酶获得的葡聚糖的性质和具有受控摩尔质量的异麦芽低聚糖和葡聚糖相比。本发明的葡聚糖和海藻酸盐可以用作变形剂或益生元。

4. 发明公开

EP1981914A2 CONSTRUCTION OF NEW VARIANTS OF DEXTRANSUCRASE DSR-S BY GENETIC ENGINEERING 有权
标题翻译：施工对基因技术的葡聚糖DSR-S的新版本
公开(公告)号：EP1981914A2
公开(公告)日：2008-10-22
申请号：EP07734270.7
申请日：2007-02-08
申请人： Centre National de la Recherche Scientifique , Institut National de Recherche Agronomique , Institut National Des Sciences Appliquees De Toulouse
发明人： MONSAN, Pierre , REMAUD-SIMEON, Magali , POTOCKI-VERONESE, Gabrielle , MOULIS, Claire
IPC分类号： C07K19/00 , C12N15/54 , C12N9/10 , C12P19/04 , C12P19/18
CPC分类号： C12N9/1051 , C07H3/06 , C07K2319/35 , C08B37/0021 , C12P19/04 , C12P19/08 , C12P19/12 , C12P19/18 , C12Y204/01005 , Y02P20/52
摘要： The present invention relates to a recombinant process for the production of truncated and/or mutated dextransucrases while conserving their enzymatic activity and/or their specificity in the synthesis of the ±-1,6 bonds. More precisely, the present invention relates to nucleic acid sequences of truncated and/or mutated dextransucrases, vectors containing said nucleic acid sequences and host cells transformed by sequences encoding truncated and/or mutated dextransucrases. In a further aspect, the invention concerns a method for producing, in a recombinant manner, truncated and/or mutated dextransucrases which conserve their enzymatic activity and/or which conserve their specificity in the synthsis of ±-1,6 bonds and however can produce, from saccharose, dextrans with high molar mass and with modified rheological properties, compared with the properties of dextran obtained with the native enzyme in the same conditions and isomalto-oligosaccharides with a controlled molar mass and dextrans. The dextrans and IMO of the invention can be used namely as texturing agents or as prebiotics.

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