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1. 发明公开

EP1967593A3 DNA typing with short tandem-repeat polymorphisms and identification of polymorphic short tandem repeats 失效
标题翻译： DNA分型短Tandemwiederholungspolymorphismen和多态短串联重复序列的识别
公开(公告)号：EP1967593A3
公开(公告)日：2008-11-12
申请号：EP08005776.3
申请日：1992-01-28
申请人： Baylor College Of Medicine
发明人： Caskey, Thomas C. , Edwards, Albert O.
IPC分类号： C12Q1/68 , C12P19/34 , C07H15/12 , G01N33/53
CPC分类号： C12Q1/6876 , B01J2219/00574 , B01J2219/00576 , B01J2219/00578 , B01J2219/00722 , C12Q1/6858 , C12Q2600/156 , C12Q2600/16 , Y10S435/81 , Y10T436/143333 , C12Q2537/143 , C12Q2531/113 , C12Q2525/151
摘要： The present invention relates to a DNA profiling assay for detecting polymorphisms in a short tandem repeat. The method includes the steps of extracting DNA from a sample to be tested, amplifying the extracted DNA and identifying the amplified extension products for each different sequence. Each different sequence is differentially labeled. In the method, internal and external standards can also be used. The method is applicable to a wide variety of forensic and medical samples, including blood, semen, vaginal swaps, tissue, hair, saliva, urine and mixtures of body fluids. A short tandem repeat sequence which can be characterized by the formula (A w G x T y C z ) n , wherein A, G, T and C represent the nucleotides, w, x, y and z represent the number of nucleotide and range from 0 to 7 and the sum of w+x+y+z ranges from 3 to 7 and n represents the repeat number and ranges from 5 to 50. The labels can be from a variety of groups, including fluorescers, radioisotopes, chemiluminescers, stains, enzymes and antibodies. Also described is a kit. Further, a method of detecting the polymorphic short tandem repeats comprising the steps of either searching for the repeats in a data base or comparing oligonucleotides and searching for the repeats in a genetic library.

2. 发明公开

EP1609875A3 DNA typing with short tandem repeat polymorphisms and identification of polymorphic short tandem repeats 失效
标题翻译： DNS“打字”短串联重复polimorphismen和短polimorphen串联重复序列的识别
公开(公告)号：EP1609875A3
公开(公告)日：2007-11-28
申请号：EP05020832.1
申请日：1992-01-28
申请人： BAYLOR COLLEGE OF MEDICINE
发明人： Caskey, Thomas C. , Edwards, Albert O.
IPC分类号： C12Q1/68 , C12P19/34 , C07H15/12 , G01N33/53
CPC分类号： C12Q1/6876 , B01J2219/00574 , B01J2219/00576 , B01J2219/00578 , B01J2219/00722 , C12Q1/6858 , C12Q2600/156 , C12Q2600/16 , Y10S435/81 , Y10T436/143333 , C12Q2537/143 , C12Q2531/113 , C12Q2525/151
摘要： The present invention relates to a DNA profiling assay for detecting polymorphisms in a short tandem repeat. The method includes the steps of extracting DNA from a sample to be tested, amplifying the extracted DNA and identifying the amplified extension products for each different sequence. Each different sequence is differentially labeled. In the method, internal and external standards can also be used. The method is applicable to a wide variety of forensic and medical samples, including blood, semen, vaginal swaps, tissue, hair, saliva, urine and mixtures of body fluids. A short tandem repeat sequence which can be characterized by the formula (A w G x T y C z ) n , wherein A, G, T and C represent the nucleotides, w, x, y and z represent the number of nucleotide and range from 0 to 7 and the sum of w+x+y+z ranges from 3 to 7 and n represents the repeat number and ranges from 5 to 50. The labels can be from a variety of groups, including fluorescers, radioisotopes, chemiluminescers, stains, enzymes and antibodies. Also described is a kit. Further, a method of detecting the polymorphic short tandem repeats comprising the steps of either searching for the repeats in a data base or comparing oligonucleotides and searching for the repeats in a genetic library.

3. 发明授权

EP1609875B9 DNA typing with short tandem repeat polymorphisms and identification of polymorphic short tandem repeats 失效
标题翻译： DNS“输入”与短串联重复Polimorphismen和短串联重复鉴定polimorphen
公开(公告)号：EP1609875B9
公开(公告)日：2011-03-02
申请号：EP05020832.1
申请日：1992-01-28
申请人： BAYLOR COLLEGE OF MEDICINE
发明人： Caskey, Thomas C. , Edwards, Albert O.
IPC分类号： C12Q1/68 , C12P19/34 , C07H15/12 , G01N33/53
CPC分类号： C12Q1/6876 , B01J2219/00574 , B01J2219/00576 , B01J2219/00578 , B01J2219/00722 , C12Q1/6858 , C12Q2600/156 , C12Q2600/16 , Y10S435/81 , Y10T436/143333 , C12Q2537/143 , C12Q2531/113 , C12Q2525/151

4. 发明公开

EP1967593A2 DNA typing with short tandem-repeat polymorphisms and identification od polymorphic short tandem repeats 失效
标题翻译：短串联重复多态性的DNA分型和多态性短串联重复序列的鉴定
公开(公告)号：EP1967593A2
公开(公告)日：2008-09-10
申请号：EP08005776.3
申请日：1992-01-28
申请人： Baylor College Of Medicine
发明人： Caskey, Thomas C. , Edwards, Albert O.
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6876 , B01J2219/00574 , B01J2219/00576 , B01J2219/00578 , B01J2219/00722 , C12Q1/6858 , C12Q2600/156 , C12Q2600/16 , Y10S435/81 , Y10T436/143333 , C12Q2537/143 , C12Q2531/113 , C12Q2525/151
摘要： The present invention relates to a DNA profiling assay for detecting polymorphisms in a short tandem repeat. The method includes the steps of extracting DNA from a sample to be tested, amplifying the extracted DNA and identifying the amplified extension products for each different sequence. Each different sequence is differentially labeled. In the method, internal and external standards can also be used. The method is applicable to a wide variety of forensic and medical samples, including blood, semen, vaginal swaps, tissue, hair, saliva, urine and mixtures of body fluids. A short tandem repeat sequence which can be characterized by the formula (A w G x T y C z ) n , wherein A, G, T and C represent the nucleotides, w, x, y and z represent the number of nucleotide and range from 0 to 7 and the sum of w+x+y+z ranges from 3 to 7 and n represents the repeat number and ranges from 5 to 50. The labels can be from a variety of groups, including fluorescers, radioisotopes, chemiluminescers, stains, enzymes and antibodies. Also described is a kit. Further, a method of detecting the polymorphic short tandem repeats comprising the steps of either searching for the repeats in a data base or comparing oligonucleotides and searching for the repeats in a genetic library.
摘要翻译：本发明涉及用于检测短串联重复中的多态性的DNA概况分析测定法。该方法包括从待测样品中提取DNA，扩增提取的DNA并鉴定每个不同序列的扩增延伸产物的步骤。每个不同的序列都有不同的标签。在该方法中，也可以使用内部和外部标准。该方法适用于各种法医和医学样品，包括血液，精液，阴道隔离物，组织，头发，唾液，尿液和体液混合物。可由式（AwGxTyCz）n表征的短串联重复序列，其中A，G，T和C表示核苷酸，w，x，y和z表示核苷酸的数量并且范围从0到7，的W + X + Y + z为3至7，n表示重复次数和范围为5〜50的标签可以是从多种基团，包括荧光剂，放射性同位素，化学发光剂，着色剂，酶和抗体。还描述了一个套件。此外，检测多态性短串联重复序列的方法包括以下步骤：搜索数据库中的重复序列或比较寡核苷酸并在遗传文库中搜索重复序列。

5. 发明授权

EP1609875B1 DNA typing with short tandem repeat polymorphisms and identification of polymorphic short tandem repeats 失效
标题翻译： DNS“输入”与短串联重复Polimorphismen和短串联重复鉴定polimorphen
公开(公告)号：EP1609875B1
公开(公告)日：2010-11-03
申请号：EP05020832.1
申请日：1992-01-28
申请人： BAYLOR COLLEGE OF MEDICINE
发明人： Caskey, Thomas C. , Edwards, Albert O.
IPC分类号： C12Q1/68 , C12P19/34 , C07H15/12 , G01N33/53
CPC分类号： C12Q1/6876 , B01J2219/00574 , B01J2219/00576 , B01J2219/00578 , B01J2219/00722 , C12Q1/6858 , C12Q2600/156 , C12Q2600/16 , Y10S435/81 , Y10T436/143333 , C12Q2537/143 , C12Q2531/113 , C12Q2525/151

6. 发明公开

EP0969103A3 Parallel primer extension approach to nucleic acid sequence analysis 失效
标题翻译：由平行的引物延伸的方法的核酸序列分析
公开(公告)号：EP0969103A3
公开(公告)日：2004-01-02
申请号：EP99200676.7
申请日：1994-06-22
申请人： Pharmacia Biotech AB , BAYLOR COLLEGE OF MEDICINE
发明人： Caskey, Thomas C. , Shumaker, John , Metspalu, Andres
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6874 , C12Q1/6858 , C12Q2531/113 , C12Q2525/186
摘要： A method of analyzing a polynucleotide of interest, comprising providing one or more sets of consecutive oligonucleotide primers differing within each set by one base at the growing end thereof; annealing a single strand of the polynucleotide or a fragment of the polynucleotide to the oligonucleotide primers under hybridization conditions; subjecting the primers to single base extension reactions with a polymerase and terminating nucleotides, the terminating nucleotides being mutually distinguishable; and observing the location and identity of each terminating nucleotide to thereby analyze the sequence or a part of the nucleotide sequence of the polynucleotide of interest, is disclosed. An apparatus comprising a solid support to which is attached at defined locations thereon one or more sets of consecutive oligonucleotide primers differing within each set by one base at the growing end thereof is also described.

7. 发明公开

EP0969103A2 Parallel primer extension approach to nucleic acid sequence analysis 失效
标题翻译： Nukleinsäure-Sequenzanalyse durch die Methode der Parallelen Primerextension
公开(公告)号：EP0969103A2
公开(公告)日：2000-01-05
申请号：EP99200676.7
申请日：1994-06-22
申请人： Pharmacia Biotech AB , BAYLOR COLLEGE OF MEDICINE
发明人： Caskey, Thomas C. , Shumaker, John , Metspalu, Andres
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6874 , C12Q1/6858 , C12Q2531/113 , C12Q2525/186
摘要： A method of analyzing a polynucleotide of interest, comprising providing one or more sets of consecutive oligonucleotide primers differing within each set by one base at the growing end thereof; annealing a single strand of the polynucleotide or a fragment of the polynucleotide to the oligonucleotide primers under hybridization conditions; subjecting the primers to single base extension reactions with a polymerase and terminating nucleotides, the terminating nucleotides being mutually distinguishable; and observing the location and identity of each terminating nucleotide to thereby analyze the sequence or a part of the nucleotide sequence of the polynucleotide of interest, is disclosed. An apparatus comprising a solid support to which is attached at defined locations thereon one or more sets of consecutive oligonucleotide primers differing within each set by one base at the growing end thereof is also described.
摘要翻译：一种分析感兴趣的多核苷酸的方法，包括在其生长末端提供一组或多组连续的寡核苷酸引物，所述引物在每组中由一个碱基不同; 在杂交条件下将多核苷酸的单链或多核苷酸的片段退火至寡核苷酸引物; 使用引物对聚合酶进行单碱基延伸反应和终止核苷酸，终止核苷酸是相互区分的; 并且观察每个终止核苷酸的位置和身份，从而分析目标多核苷酸的序列或部分核苷酸序列。还描述了一种包括固体支持物的装置，其中在其一定位置附着有一组或多组连续的寡核苷酸引物，其在每一组中在其生长端处由一个碱基不同。

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