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1. 发明授权

EP1383732B1 REACTIFS DE MARQUAGE, PROCEDES DE SYNTHESE DE TELS REACTIFS ET PROCEDES DE DETECTION DE MOLECULES BIOLOGIQUES 有权
标题翻译：标记试剂，处理此试剂用于检测生物分子的合成和方法
公开(公告)号：EP1383732B1
公开(公告)日：2010-02-17
申请号：EP02740788.1
申请日：2002-05-03
申请人： BIO MERIEUX , UNIVERSITE JOSEPH FOURIER (GRENOBLE 1) , CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE (CNRS)
发明人： BOURGET, Cécile , LHOMME, Jean , LAAYOUN, Ali , KOTERA, Mitsuharu , TREVISIOL, Emmanuelle , MENOU, Lionel , BERNAL MENDEZ, Eloy
IPC分类号： C07C245/14 , C07C245/18 , C07D209/14 , C07D495/04 , C07D311/82 , C12Q1/68
CPC分类号： C07D209/08 , C07C245/14 , C07C245/18 , C07D311/90 , C07D495/04

2. 发明授权

EP1383925B1 PROCEDE DE MARQUAGE ET DE FRAGMENTATION D'ADN 有权
标题翻译：方法标记和DNA片段化
公开(公告)号：EP1383925B1
公开(公告)日：2006-09-20
申请号：EP02738211.8
申请日：2002-05-03
申请人： BIO MERIEUX , UNIVERSITE JOSEPH FOURIER (GRENOBLE 1) , CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE
发明人： BOURGET, Cécile , KOTERA, Mitsuharu , LHOMME, Jean , TREVISIOL, Emmanuelle , LAAYOUN, Ali , TORA, Christelle , SOTHIER, Isabelle
IPC分类号： C12Q1/68
CPC分类号： C12Q1/68
摘要： The invention concerns a method for labelling and fragmenting a single-stranded or double-stranded deoxyribonucleic acid (DNA) comprising the following steps: chemically fragmenting the DNA by generating an abasic site on said DNA, attaching a marker on at least one of the fragments by means of a labelling reagent, said reagent being covalently and majority bonded on at least one phosphate of said fragment. The invention is applicable in the field of diagnosis.

3. 发明公开

EP1383925A2 PROCEDE DE MARQUAGE ET DE FRAGMENTATION D'ADN 有权
标题翻译：方法标记和DNA片段化
公开(公告)号：EP1383925A2
公开(公告)日：2004-01-28
申请号：EP02738211.8
申请日：2002-05-03
申请人： BIO MERIEUX , UNIVERSITE JOSEPH FOURIER (GRENOBLE 1) , Centre National De La Recherche Scientifique
发明人： BOURGET, Cécile , KOTERA, Mitsuharu , LHOMME, Jean , TREVISIOL, Emmanuelle , LAAYOUN, Ali , TORA, Christelle , SOTHIER, Isabelle
IPC分类号： C12Q1/68
CPC分类号： C12Q1/68
摘要： The invention concerns a method for labelling and fragmenting a single-stranded or double-stranded deoxyribonucleic acid (DNA) comprising the following steps: chemically fragmenting the DNA by generating an abasic site on said DNA, attaching a marker on at least one of the fragments by means of a labelling reagent, said reagent being covalently and majority bonded on at least one phosphate of said fragment. The invention is applicable in the field of diagnosis.

4. 发明公开

EP1383732A1 REACTIFS DE MARQUAGE, PROCEDES DE SYNTHESE DE TELS REACTIFS ET PROCEDES DE DETECTION DE MOLECULES BIOLOGIQUES 有权
标题翻译：标记试剂，处理此试剂用于检测生物分子的合成和方法
公开(公告)号：EP1383732A1
公开(公告)日：2004-01-28
申请号：EP02740788.1
申请日：2002-05-03
申请人： BIO MERIEUX , UNIVERSITE JOSEPH FOURIER (GRENOBLE 1) , CENTRE NATIONAL DELA RECHERCHE SCIENTIFIQUE (CNRS)
发明人： BOURGET, Cécile , LHOMME, Jean , LAAYOUN, Ali , KOTERA, Mitsuharu , TREVISIOL, Emmanuelle , MENOU, Lionel , BERNAL MENDEZ, Eloy
IPC分类号： C07C245/14 , C07C245/18 , C07D209/14 , C07D495/04 , C07D311/82 , C12Q1/68
CPC分类号： C07D209/08 , C07C245/14 , C07C245/18 , C07D311/90 , C07D495/04
摘要： The invention concerns a temperature-stable labelling reagent of formula (I), wherein: R1 represents H or an alkyl, aryl or substituted aryl group; R2 represents a detectable marker or at least two detectable markers linked together by at least a multimeric structure; L is a linking arm comprising a linear catemer concatenation of at least two covalent bonds; and n is an integer equal to 0 or 1; R?3 and R4¿ represent, independently of each other: H, NO¿2?, Cl, Br, F, I, R?2-(L)¿n-Y-X-, OR, SR, NR2, R, NHCOR, CONHR, COOR with R = alkyl or aryl, A is a linking arm comprising at least a double bond enabling the conjugation of the diazo function with the aromatic cycle; and u is a whole number between 0 and 2, preferably between 0 and 1; and -Y-X- represents -CONH-, -NHCO-, -CH2O-, -CH2S-. The invention also concerns a method for the synthesis of said markers and uses for labelling biological molecules, in particular nucleic acids, with a labelling reagent bearing the diazomethyl function. The invention is particularly applicable in the field of diagnosis.

5. 发明公开

EP1973927A2 CATIONIC OLIGONUCLEOTIDES, AUTOMATED METHODS FOR PREPARING SAME AND THEIR USES 有权
标题翻译：阳离子寡核苷酸，自动化的过程作为生产，用途
公开(公告)号：EP1973927A2
公开(公告)日：2008-10-01
申请号：EP06847298.4
申请日：2006-12-14
申请人： Centre National De La Recherche Scientifique-CNRS , PolyPlus Transfection
发明人： BEHR, Jean-Paul , KOTERA, Mitsuharu , PONS, Bénédicte , VOIRIN, Emilie , REMY, Jean-Serge
IPC分类号： C07H21/00
CPC分类号： C07F9/22 , A61K47/59 , A61K48/0041 , C07H21/00 , C12N15/88
摘要： The invention relates to oligonucleotide-oligocation molecules AiBjH that can be synthetized via automated phosphoramidite chemistry having oligonucleotides moieties Ai and oligocations moieties Bj, wherein . Ai is an i-mer oligonucleotide residue, with i = 5 to 50, where nucleotide A is an oligomer with naturally or non naturally occurring nucleobases and/or pentafuranosyl groups and/or native phosphodiester bonds, for example selected from the group comprising deoxyribo, ribo, locked (LNA) nucleotides as well as their chemical modifications or substitutions such as phosphorothioate, 2'-fluoro, 2'-O-alkyl, or a marker group such as a fluorescent agent, . Bj is a j-mer organic oligocation moiety, with j = 1 to 50, where B is selected from the group comprising • - HPO3-R1 -(X-R2n)n1-X-R3-O-, where R1, R2n and R3, identical or different, are lower alkylene, X is NH or NC(NH2)2, n varies from 1 to 5 and n1 = 2 to 20, • - HPO3-R4-CH(R5X1)-R6-O-, where R4 is lower alkylene, R5 and R6, identical or different, are lower alkylene and X1 is putrescine, spermidine or spermine residue, • - HPO3-R7-(aa)n2-R8-O-, where R7 is lower alkylene and R8 is lower alkylene, serine, a natural aminoalcohol, (aa)n2 is a peptide containing natural aminoacids with cationic side chains, such as Arginine, Lysine, Ornithine, - Histidine, Diaminopropionic acid and n2= 2 to 20.
摘要翻译：本发明涉及的寡核苷酸 - 寡聚阳离子分子AiBjH确实可以经由具有寡核苷酸部分A i和寡阳离子部分BJ自动化亚磷酰胺化学synthetized，worin•艾是i聚体寡核苷酸残基，其中i = 5〜50，其中核苷酸A是低聚与选自天然或非天然存在的核碱基和/或pentafuranosyl基团和/或天然磷酸二酯键，例如包括脱氧核糖，核糖，锁定（LNA）核苷酸以及其化学修饰或取代：例如硫代磷酸酯，2'- 氟，2'-O-烷基或标记基团：如荧光剂，•BJ是第j聚体有机寡聚阳离子部分，其中j = 1至50，其中B选自包括以下的组•-HPO3-R1选择 - （X-R 2n）后N1-X-R3-O-，其中R 1，R 2 N和R 3，相同或不同，是低级亚烷基，X是NH或NC（NH 2）2，N从1变化至5并且n1 = 2 至20，•-HPO3-R4-CH（R5X1）-R 6 -O-，其中R 4是低级亚烷基，R5和R6，相同或不同，是低级亚烷基和X 1为腐胺，亚精胺或精胺残基，•-HPO3-R7（AA）N2-R8-O-，其中R 7是低级亚烷基和R 8是低级亚烷基，丝氨酸，天然氨基醇（AA）n2是含有具有阳离子侧链的,:如精氨酸，赖氨酸，鸟氨酸，组氨酸，二氨基丙酸和n2 = 2到20的自然氨基酸的肽

6. 发明授权

EP1973927B1 CATIONIC OLIGONUCLEOTIDES, AUTOMATED METHODS FOR PREPARING SAME AND THEIR USES 有权
标题翻译：阳离子寡核苷酸，自动化的过程作为生产，用途
公开(公告)号：EP1973927B1
公开(公告)日：2013-08-14
申请号：EP06847298.4
申请日：2006-12-14
申请人： Centre National de la Recherche Scientifique - CNRS , PolyPlus Transfection
发明人： BEHR, Jean-Paul , KOTERA, Mitsuharu , PONS, Bénédicte , VOIRIN, Emilie , REMY, Jean-Serge
IPC分类号： C07H21/00
CPC分类号： C07F9/22 , A61K47/59 , A61K48/0041 , C07H21/00 , C12N15/88

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