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1. 发明公开

EP4112741A1 STEPWISE SEQUENCING BY NON-LABELED REVERSIBLE TERMINATORS OR NATURAL NUCLEOTIDES 审中-公开
公开(公告)号：EP4112741A1
公开(公告)日：2023-01-04
申请号：EP22174722.3
申请日：2018-01-04
申请人： MGI Tech Co., Ltd. , BGI Shenzhen
发明人： DRMANAC, Radoje , DRMANAC, Snezana , LI, Handong , XU, Xun , CALLOW, Matthew J. , ECKHARDT, Leon , YANG, Naibo
IPC分类号： C12Q1/6804 , C12Q1/6869 , C07K16/44 , G01N33/53
摘要： The invention provides compositions and methods for sequencing nucleic acids and other applications. In sequencing by synthesis, unlabeled reversible terminators are incorporated by a polymerase in each cycle, then labeled after incorporation by binding to the reversible terminator a directly or indirectly labeled antibody or other affinity reagent.

2. 发明公开

EP3877548A1 MASSIVELY PARALLEL SEQUENCING USING UNLABELED NUCLEOTIDES 审中-公开
公开(公告)号：EP3877548A1
公开(公告)日：2021-09-15
申请号：EP19881829.6
申请日：2019-11-11
申请人： MGI Tech Co., Ltd. , BGI Shenzhen
发明人： DRMANAC, Snezana , LI, Handong , CALLOW, Matthew, J. , ECKHARDT, Leon , GABLENZ, Scott , DRMANAC, Radoje , ZHOU, Ping
IPC分类号： C12Q1/6874 , C12Q1/6876

3. 发明公开

EP3971282A1 ARRAY AND METHOD FOR DETECTING SPATIAL INFORMATION OF NUCLEIC ACIDS 审中-实审
公开(公告)号：EP3971282A1
公开(公告)日：2022-03-23
申请号：EP20806227.3
申请日：2020-05-14
申请人： BGI Shenzhen , MGI Tech Co., Ltd.
发明人： CHEN, Ao , XU, Xun , YANG, Jin , LIU, Longqi , WANG, Ou , LI, Yuxiang , LIAO, Sha , TANG, Guoxin , JIANG, Yuan , XU, Chongjun , NI, Ming , ZHANG, Wenwei , DRMANAC, Radoje , DRMANAC, Snezana
IPC分类号： C12M1/34 , C12Q1/68 , C40B40/06 , C40B20/02
摘要： Provided are a method for detecting spatial information of nucleic acids in a sample, as well as a nucleic acid array used in the method and a method for producing the nucleic acid array.

4. 发明公开

EP3565905A1 STEPWISE SEQUENCING BY NON-LABELED REVERSIBLE TERMINATORS OR NATURAL NUCLEOTIDES 有权
公开(公告)号：EP3565905A1
公开(公告)日：2019-11-13
申请号：EP18736171.2
申请日：2018-01-04
申请人： MGI Tech Co., Ltd. , BGI Shenzhen
发明人： DRMANAC, Radoje , DRMANAC, Snezana , LI, Handong , XU, Xun , CALLOW, Matthew J. , ECKHARDT, Leon , YANG, Naibo , DING, Quan
IPC分类号： C12Q1/6804 , C12Q1/6869 , C07K16/44 , G01N33/53

5. 发明公开

EP3730613A1 HOOKED PROBE, METHOD FOR LIGATING NUCLEIC ACID AND METHOD FOR CONSTRUCTING SEQUENCING LIBRARY 审中-公开
公开(公告)号：EP3730613A1
公开(公告)日：2020-10-28
申请号：EP17931336.6
申请日：2017-11-09
申请人： MGI Tech Co., Ltd.
发明人： JIANG, Yuan , XI, Yang , ZHANG, Wenwei , LIU, Pengjuan , ZHAO, Xia , LI, Qiaoling , SHEN, Hanjie , ZHANG, Yongwei , DRMANAC, Radoje
IPC分类号： C12N15/10 , C12Q1/68
摘要： Provided are a hooked probe, a method for ligating a nucleic acid and a method for constructing a sequencing library. The hooked probe comprises a target specific area and a hooked area ligated thereto; the target specific area comprises a sequence complementarily paired with at least part of the single chain of the nucleic acid fragment to be ligated; the hooked area comprises a sequence unpaired with the nucleic acid fragment; the end of the hooked area is a ligatable end; and the ligatable end can ligate the end of the single chain of the nucleic acid fragment.

6. 发明授权

EP3388519B1 VESICULAR ADAPTOR AND USES THEREOF IN NUCLEIC ACID LIBRARY CONSTRUCTION AND SEQUENCING 有权
公开(公告)号：EP3388519B1
公开(公告)日：2019-07-10
申请号：EP18174793.2
申请日：2014-11-21
申请人： MGI Tech Co., Ltd.
发明人： JIANG, Yuan , GUO, Jing , JI, Xiaojun , GENG, Chunyu , TIAN, Kai , ZHAO, Xia , XU, Huaiqian , ZHANG, Wenwei , JIANG, Hui , DRMANAC, Radoje
IPC分类号： C12N15/10 , C12N15/66 , C12Q1/68 , C40B40/08 , C40B50/06

7. 发明公开

EP3225721A1 METHOD AND REAGENT FOR CONSTRUCTING NUCLEIC ACID DOUBLE-LINKER SINGLE-STRAND CYCLICAL LIBRARY 审中-公开
标题翻译：构建核酸双链单链循环库的方法和试剂
公开(公告)号：EP3225721A1
公开(公告)日：2017-10-04
申请号：EP14907081.5
申请日：2014-11-26
申请人： BGI Shenzhen , BGI Shenzhen Co., Limited
发明人： JIANG, Yuan , ZHAO, Xia , ALEXEEV, Andrei , DRMANAC, Radoje , ZHANG, Wenwei , JIANG, Hui
IPC分类号： C40B50/06 , C40B50/18 , C12N15/11 , C12N15/10 , C12Q1/68
CPC分类号： C12N15/1068 , C12N15/10 , C12N15/11 , C12Q1/68 , C40B50/06 , C40B50/18
摘要： A method and reagent for constructing a nucleic acid double-joint single-strand cyclical library. The method comprises: breaking a nucleic acid into nucleic acid fragments; connecting a first linker sequence; producing by amplification a first product provided with the first linker sequence at either end, where a U nucleobase is provided on a primer sequence; using USER enzyme to cleave the first product and cyclizing to produce a gap; or, a nicking enzyme recognition sequence is also provided on the primer sequence, using the USER enzyme to cleave the first product, cyclizing and using a nicking enzyme for nicking to produce a nick; performing a restrictive nick/gap translation reaction from the nick or the gap; removing by digestion any portion that did not undergo the restrictive nick/gap translation reaction; connecting a second linker sequence; producing by amplification a second product provided with the second linker sequence at either end; denaturing the second product, and using a mediated sequence for cyclization of a single-strand nucleic acid molecule. The method allows an increase in the length of library insert fragments and obviates the need for gel extraction; the single-strand nucleic acid molecule can be cyclized directly when denatured with heat.
摘要翻译：构建核酸双联单链循环文库的方法和试剂。该方法包括：将核酸分解成核酸片段; 连接第一连接序列; 通过扩增产生在任一末端提供有第一接头序列的第一产物，其中在引物序列上提供U核碱基; 使用USER酶裂解第一种产物并环化以产生间隙; 或者，在引物序列上还提供切口酶识别序列，使用USER酶切割第一种产物，环化并使用切口酶进行切口以产生切口; 从缺口或间隙执行限制性缺口/缺口翻译反应; 通过消化除去未经历限制性切口/间隙翻译反应的任何部分; 连接第二连接序列; 通过扩增产生在任一端提供有第二接头序列的第二产物; 使第二种产物变性，并使用介导的序列使单链核酸分子环化。该方法允许增加文库插入片段的长度并且不需要凝胶提取; 单链核酸分子可以在加热变性时直接环化。

8. 发明公开

EP3315461A1 MICRO-POROUS ELECTRODE AND METHOD FOR ANALYSIS OF CHEMICAL SUBSTANCES 有权
标题翻译：微孔电极和用于分析化学物质的方法
公开(公告)号：EP3315461A1
公开(公告)日：2018-05-02
申请号：EP16813709.9
申请日：2016-06-23
申请人： BGI Shenzhen , BGI Shenzhen Co., Limited
发明人： LI, Handong , LIN, Jianxun , YUN, Quanxin , XIANG, Shaohua , DRMANAC, Radoje , DRMANAC, Snezana , ZHANG, Yongwei
IPC分类号： B81B7/02 , C12Q1/68
CPC分类号： G01N27/3276 , B81B7/02 , C12Q1/68 , C12Q1/6869 , G01N27/3277 , G01N27/3278
摘要： Provided is a microwell electrode, comprising one or more first electrodes (301); one or more second electrodes (303) each arranged opposite to one first electrode (301), wherein a channel (601) is provided between each first electrode and the second electrode opposite thereto, and the channel (601) has at least one end in communication with a chamber; and one or more guiding electrodes (501) located in the chamber (401). The microwell electrode electrode can sensitively detect a signal and improve the read length of a sequencer greatly. The invention further relates to a method for manufacturing the micro-porous electrode, a microwell electrode array, a sensor chip, a sequencing system, and a method for analysis of a chemical substance and a nucleic acid molecule based on the microwell electrode.
摘要翻译：提供了一种微孔电极，包括一个或多个第一电极（301）; 一个或多个第二电极（303），每个第二电极与一个第一电极（301）相对布置，其中在每个第一电极和与其相对的第二电极之间提供沟道（601），并且沟道（601）至少具有一端与商会沟通; 以及位于腔室（401）中的一个或多个引导电极（501）。微孔电极可以灵敏地检测信号，大大提高了测序仪的读数长度。本发明还涉及用于制造微孔电极的方法，微孔电极阵列，传感器芯片，测序系统以及基于微孔电极的用于分析化学物质和核酸分子的方法。

9. 发明公开

EP4190910A1 METHOD FOR LOADING NUCLEIC ACID MOLECULE ON SOLID SUPPORT 审中-公开
公开(公告)号：EP4190910A1
公开(公告)日：2023-06-07
申请号：EP20947567.2
申请日：2020-07-29
申请人： MGI Tech Co., Ltd.
发明人： SHAFTO, Jay , DRMANAC, Radoje , DRMANAC, Snezana , XU, Chongjun , GONG, Meihua , WANG, Ping , LONG, Xiaojuan , ZHAO, Wei , LUO, Huan , JIANG, Hui , LIU, Jian
IPC分类号： C12Q1/68 , C40B50/18
摘要： Provided are a method for loading a nucleic acid molecule, for example nucleic acid nanoball (e.g., DNA nanoball (DNB)), on a solid support, and a kit for the method

10. 发明公开

EP4081652A1 CONTROLLED STRAND-DISPLACEMENT FOR PAIRED-END SEQUENCING 审中-实审
公开(公告)号：EP4081652A1
公开(公告)日：2022-11-02
申请号：EP20906868.3
申请日：2020-12-23
申请人： MGI Tech Co., Ltd.
发明人： DRMANAC, Radoje , CALLOW, Matthew J.
IPC分类号： C12Q1/68

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