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1. 发明公开

EP2716764A4 CONSTRUCTION AND APPLICATION OF MUTANT TYPE B HUMAN ADENOVIRUS AD11 HAVING ENHANCED ONCOLYTIC POWER 有权
标题翻译：建设和突变的人类腺病毒AD11 TYPE B的性能得到改善溶瘤应用
公开(公告)号：EP2716764A4
公开(公告)日：2015-01-14
申请号：EP12792810
申请日：2012-02-29
申请人： BEIJING BIO TARGETING THERAPEUTICS TECHNOLOGY INC
发明人： WANG YAOHE , JIANG HANSHI , WONG HANSHI , CAO FENGYU , LEMOINE NICK
IPC分类号： C12N15/861 , A61K48/00 , A61P35/00 , C12N15/66 , C12R1/93
CPC分类号： C12N15/86 , A61K35/761 , C12N7/00 , C12N2710/10321 , C12N2710/10332 , C12N2710/10343
摘要： A method for constructing type-B recombinant human adenoviral vectors Ad11-5EP and Ad11-5ETel-GFP, and applications thereof. Construction of the adenoviral vector Ad11-5EP utilizes homologous recombination to substitute a 365 bp-long gene upstream segment of the Ad5 E1A coding sequence (comprising the enhancer and promoter of Ad5 E1A) to the corresponding region of type-B human adenovirus subtype 11 Ad11, thereby constructing said vector Ad11-5EP. The method for constructing the type-B recombinant human adenoviral vectorAd11-5ETel-GFP is simultaneous homologous recombination of the genomes of the constructed shuttle vectors pSSENTel, pSSGFP and Ad11-5EP, so as to produce said Ad11-5ETel-GFP. Vector Ad11-5EP possesses a stronger oncolytic effect than the wild-type Ad11, which enhances the power to kill tumor cells. Measurements of tumour growth and tumour clearance rates show that Ad11-5EP effectively reduces tumour growth rates, while the tumour-free ratio among tumour-bearing mice using AdII-5EP is noticeably better than for Ad11. Constructed Ad11-SETel-GFP can be used for treating tumours, or for specific, sensitive and economical detection of tumour cells in circulating blood.
摘要翻译：一种用于构造类型B的重组人腺病毒载体Ad11-5EP和Ad11-5ETel-GFP，和其应用方法。所述腺病毒载体Ad11-5EP的构建利用同源重组以替代的Ad5 E1A的365 bp的基因长上游区段的编码序列（包括Ad5的E1A的增强子和启动子）以B型人类腺病毒亚型11 AD11的相应区域，从而构建所述载体Ad11-5EP。构建B型重组人腺病毒vectorAd11-5ETel-GFP的方法是构建穿梭的基因组的同时同源重组载体pSSENTel，pSSGFP和Ad11-5EP以便产生所述Ad11-5ETel GFP。矢量Ad11-5EP拥有比野生型AD11，从而增强杀死肿瘤细胞的动力更强的杀伤效果。的肿瘤生长和肿瘤清除率测量显示没有Ad11-5EP有效地降低肿瘤的生长速率，同时使用ADII-5EP荷瘤小鼠中的无肿瘤比率明显比为AD11更好。构造AD11-SETEL-GFP可以用于治疗肿瘤，或用于循环血液的肿瘤细胞的特异性的，灵敏的和经济的检测。

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