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    • 8. 发明公开
    • GLUCAN AND MANNAN, THEIR PREPARATION METHOD AND USE
    • GLANAN UND MANNAN,HERSTELLUNGSVERFAHRENDAFÜRUND VERWENDUNG DAVON
    • EP2272876A1
    • 2011-01-12
    • EP08874088.1
    • 2008-12-31
    • Angel Yeast Co., Ltd.
    • YU, XuefengLI, ZhihongYU, MinghuaYAO, JuanZHANG, Yan
    • C08B37/00C12P19/04
    • C12P19/04C08B37/0003C08B37/0087
    • The present invention provides a method for extracting glucan and mannan from the cell wall of a microorganism. Specifically, the method of the present invention in one embodiment comprises the steps of: a) treating the cells of the microorganism with an alkaline protease and an mannanase; b) separating the mixture from step a) into a heavy phase and a light phase; c) drying the heavy phase obtained from step b), obtaining the glucan preparation; and d) drying the light phase obtained from step b), obtaining the mannan preparation. Optionally, in the step c), the heavy phase obtained from step b) may be treated sequentially with an alkali and an acid, and separated again into a heavy phase and a light phase. The heavy phase is dried, obtaining the glucan preparation. The present invention further relates to the glucan preparation and mannan preparation produced thereby, and the uses thereof.
    • 本发明提供从微生物细胞壁提取葡聚糖和甘露聚糖的方法。 具体地说,本发明的一个实施方案包括以下步骤:a)用碱性蛋白酶和甘露聚糖酶处理微生物细胞; b)将步骤a)中的混合物分离成重相和轻相; c)干燥从步骤b)获得的重相,得到葡聚糖制剂; 和d)干燥从步骤b)得到的轻相,得到甘露聚糖制剂。 任选地,在步骤c)中,可以用碱和酸依次处理从步骤b)获得的重相,并再次分离成重相和轻相。 将重相干燥,得到葡聚糖制剂。 本发明还涉及由此制备的葡聚糖制剂和甘露聚糖制剂及其用途。