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1. 发明公开

EP3313996A1 MALTOSE DEPENDENT DEGRONS, MALTOSE-RESPONSIVE PROMOTERS, STABILIZATION CONSTRUCTS, AND THEIR USE IN PRODUCTION OF NON-CATABOLIC COMPOUNDS 审中-公开
标题翻译：麦芽糖依赖性的麦芽糖，麦芽糖响应的促进剂，稳定化结构及其在生产非催化性化合物中的用途
公开(公告)号：EP3313996A1
公开(公告)日：2018-05-02
申请号：EP16739303.2
申请日：2016-06-24
申请人： Amyris, Inc. , Total Marketing Services
发明人： CHUA, Penelope R. , JIANG, Hanxiao , MEADOWS, Adam Leon
IPC分类号： C12P5/02 , C12P7/64 , C12P19/62 , C12P21/00
CPC分类号： C07K14/395 , C07K2319/95 , C12N1/16 , C12N15/52 , C12N15/635 , C12N15/65 , C12N2330/51 , C12P5/02 , C12P5/026 , C12P7/64 , C12P19/62 , C12P21/00 , C12P21/02 , C12Y503/03002
摘要： The present disclosure relates to the use of a maltose dependent degron to control stability of a protein of interest fused thereto at the post-translational level. The present disclosure also relates to the use of a maltose dependent degron in combination with a maltose-responsive promoter to control gene expression at the transcriptional level and to control protein stability at the post-translational level. The present disclosure also relates to the use of a stabilization construct that couples expression of a cell-growth-affecting protein with the production of non-catabolic compounds. The present disclosure further relates to the use of a synthetic maltose-responsive promoter. The present disclosure further provides compositions and methods for using a maltose dependent degron, a maltose-responsive promoter, and a stabilization construct, either alone or in various combinations, for the production of non-catabolic compounds in genetically modified host cells.

2. 发明公开

EP3313995A1 MALTOSE DEPENDENT DEGRONS, MALTOSE-RESPONSIVE PROMOTERS, STABILIZATION CONSTRUCTS, AND THEIR USE IN PRODUCTION OF NON-CATABOLIC COMPOUNDS 有权
公开(公告)号：EP3313995A1
公开(公告)日：2018-05-02
申请号：EP16734852.3
申请日：2016-06-24
申请人： Amyris, Inc. , Total Marketing Services
发明人： CHUA, Penelope R. , JIANG, Hanxiao , MEADOWS, Adam Leon
IPC分类号： C12P5/02 , C12P7/64 , C12P19/62 , C12P21/00
CPC分类号： C07K14/395 , C07K2319/95 , C12N1/16 , C12N15/52 , C12N15/635 , C12N15/65 , C12N2330/51 , C12P5/02 , C12P5/026 , C12P7/64 , C12P19/62 , C12P21/00 , C12P21/02 , C12Y503/03002
摘要： The present disclosure relates to the use of a maltose dependent degron to control stability of a protein of interest fused thereto at the post-translational level. The present disclosure also relates to the use of a maltose dependent degron in combination with a maltose-responsive promoter to control gene expression at the transcriptional level and to control protein stability at the post-translational level. The present disclosure also relates to the use of a stabilization construct that couples expression of a cell-growth-affecting protein with the production of non-catabolic compounds. The present disclosure further relates to the use of a synthetic maltose-responsive promoter. The present disclosure further provides compositions and methods for using a maltose dependent degron, a maltose-responsive promoter, and a stabilization construct, either alone or in various combinations, for the production of non-catabolic compounds in genetically modified host cells.

3. 发明公开

EP2971027A2 USE OF PHOSPHOKETOLASE AND PHOSPHOTRANSACETYLASE FOR PRODUCTION OF ACETYL-COENZYME A DERIVED COMPOUNDS 有权
标题翻译：面对化合物从乙酰辅酶A的生产中使用和磷酸磷酸
公开(公告)号：EP2971027A2
公开(公告)日：2016-01-20
申请号：EP14714149.3
申请日：2014-03-14
申请人： Amyris, Inc. , Total Marketing Services
发明人： HAWKINS, Kristy Michelle , MAHATDEJKUL-MEADOWS, Tina Tipawan , MEADOWS, Adam Leon , PICKENS, Lauren Barbara , TAI, Anna , TSONG, Annie Ening
IPC分类号： C12P19/00 , C12P23/00 , C12P33/00 , C12P5/00 , C12P7/00 , C12P15/00 , C12P17/00 , C12N9/10 , C12N9/16 , C12N9/88
CPC分类号： C12Y401/02009 , C12N9/1029 , C12N9/16 , C12N9/88 , C12P5/007 , C12P7/54 , C12Y203/01008 , C12Y301/03021 , Y02P20/52
摘要： Provided herein are compositions and methods for improved production of acetyl-CoA and acetyl-CoA derived compounds in a host cell. In some embodiments, the host cell is genetically modified to comprise a heterologous nucleotide sequence encoding a phosphoketolase (PK), and a functional disruption of an endogenous enzyme that converts acetyl phosphate to acetate. In some embodiments, the host cell further comprises a heterologous nucleotide sequence encoding a phosphotransacetylase (PTA). In some embodiments, the enzyme that converts acetyl phosphate to acetate is a glycerol-1-phosphatase. In some embodiments, the glycerol-1-phosphatase is GPP1/RHR2. In some embodiments, the glycerol-1-phosphatase is GPP2/HOR2. The compositions and methods described herein provide an efficient route for the heterologous production of acetyl-CoA-derived compounds, including but not limited to, isoprenoids, polyketides, and fatty acids.

4. 发明公开

EP2776571A1 PRODUCTION OF ACETYL-COENZYME A DERIVED ISOPRENOIDS 有权
标题翻译： UNG ON ON ON EN EN EN EN EN EN EN EN EN EN EN EN EN EN EN EN
公开(公告)号：EP2776571A1
公开(公告)日：2014-09-17
申请号：EP12795948.4
申请日：2012-11-09
申请人： Amyris, Inc.
发明人： GARDNER, Timothy Stevens , HAWKINS, Kristy Michelle , MEADOWS, Adam Leon , TSONG, Annie Ening , TSEGAYE, Yoseph
IPC分类号： C12P23/00 , C12P5/00 , C12N15/52
CPC分类号： C12P5/007 , C12N9/0006 , C12N9/0008 , C12N9/1288 , C12N15/52 , C12N15/81 , C12P7/42 , C12P23/00 , C12Y101/01034 , C12Y102/0101 , C12Y207/08
摘要： Provided herein are compositions and methods for the heterologous production of acetyl-CoA-derived isoprenoids in a host cell. In some embodiments, the host cell is genetically modified to comprise a heterologous nucleotide sequence encoding an acetaldehyde dehydrogenase, acetylating (ADA, E.C. 1.2.1.10) and an MEV pathway comprising an NADH-using HMG-CoA reductase. In some embodiments, the host cell is genetically modified to comprise a heterologous nucleotide sequence encoding an ADA and an MEV pathway comprising an acetoacetyl-CoA synthase. In some embodiments, the genetically modified host cell further comprises one or more heterologous nucleotide sequences encoding a phosphoketolase and a phosphotransacetylase. In some embodiments, the genetically modified host cell further comprises a functional disruption of the native PDH-bypass. The compositions and methods described herein provide an energy-efficient yet redox balanced route for the heterologous production of acetyl-CoA-derived isoprenoids.
摘要翻译：本文提供了在宿主细胞中异源产生乙酰辅酶A衍生的类异戊二烯的组合物和方法。在一些实施方案中，宿主细胞经遗传修饰以包含编码乙醛脱氢酶的异源核苷酸序列，乙酰化（ADA，E.C.1.2.1.10）和包含使用NADH的HMG-CoA还原酶的MEV途径。在一些实施方案中，宿主细胞被遗传修饰以包含编码包含乙酰乙酰辅酶A合成酶的ADA和MEV途径的异源核苷酸序列。在一些实施方案中，经遗传修饰的宿主细胞还包含一个或多个编码磷酸酮醇酶和磷酸转乙酰酶的异源核苷酸序列。在一些实施方案中，遗传修饰的宿主细胞还包括天然PDH旁路的功能性破坏。本文描述的组合物和方法提供了用于异源产生乙酰辅酶A衍生的类异戊二烯的能量效率高的均衡路线。

6. 发明授权

EP2776571B1 PRODUCTION OF ACETYL-COENZYME A DERIVED ISOPRENOIDS 有权
标题翻译：生产乙酰辅酶A第二类异戊二烯
公开(公告)号：EP2776571B1
公开(公告)日：2017-04-12
申请号：EP12795948.4
申请日：2012-11-09
申请人： Amyris, Inc.
发明人： GARDNER, Timothy Stevens , HAWKINS, Kristy Michelle , MEADOWS, Adam Leon , TSONG, Annie Ening , TSEGAYE, Yoseph
IPC分类号： C12P23/00 , C12P5/00 , C12N15/52
CPC分类号： C12P5/007 , C12N9/0006 , C12N9/0008 , C12N9/1288 , C12N15/52 , C12N15/81 , C12P7/42 , C12P23/00 , C12Y101/01034 , C12Y102/0101 , C12Y207/08
摘要： Provided herein are compositions and methods for the heterologous production of acetyl-CoA-derived isoprenoids in a host cell. In some embodiments, the host cell is genetically modified to comprise a heterologous nucleotide sequence encoding an acetaldehyde dehydrogenase, acetylating (ADA, E.C. 1.2.1.10) and an MEV pathway comprising an NADH-using HMG-CoA reductase. In some embodiments, the host cell is genetically modified to comprise a heterologous nucleotide sequence encoding an ADA and an MEV pathway comprising an acetoacetyl-CoA synthase. In some embodiments, the genetically modified host cell further comprises one or more heterologous nucleotide sequences encoding a phosphoketolase and a phosphotransacetylase. In some embodiments, the genetically modified host cell further comprises a functional disruption of the native PDH-bypass. The compositions and methods described herein provide an energy-efficient yet redox balanced route for the heterologous production of acetyl-CoA-derived isoprenoids.

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