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    • 5. 发明公开
    • Stabilised enzyme dispersion
    • Stabilisierte Enzymdispersion。
    • EP0351162A1
    • 1990-01-17
    • EP89306974.0
    • 1989-07-10
    • Albright & Wilson LimitedNOVO NORDISK A/S
    • Hawkins, JohnChadwick, PhilipMessenger, Edward TunstallLykke, Mads
    • C11D3/386C12N9/96
    • C11D3/38663
    • A convenient method for preparing a stabilized aqueous enzyme dispersion comprises:

      (1) precipitating a water-soluble polymer from aqueous solution to form an aqueous dispersion, and
      (2) before, during or after (1), contacting the dissolved or dispersed polymer with an aqueous solution or fine aqueous dispersion of enzyme.

      Using this method, substantial improvement of the enzyme stability during storage can be obtained with surpris­ingly little polymer (relative to enzyme). And enzyme stabilization can, surprisingly, even be obtained by contact­ing precipitated polymer with dissolved enzyme. The stabiliz­ing effect therefore appears not to be due (or at least not primarily due) to encapsulation.
    • 制备稳定的含水酶分散体的方便方法包括:(1)从水溶液中沉淀出水溶性聚合物以形成水分散体,和(2)在(1)之前,之前或之后,将溶解或分散的聚合物与 酶的水溶液或细水分散体。 使用这种方法,可以获得令人惊讶的少量聚合物(相对于酶)在储存期间显着提高酶稳定性。 令人惊讶的是,甚至可以通过使沉淀的聚合物与溶解的酶接触来获得酶稳定化。 因此,稳定效应似乎不是由于(或至少不是主要是由于)封装。