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1. 发明公开

EP1130117A2 Method and device for detection of nucleic acid using total internal reflectance 失效
标题翻译：为使用全内反射的核酸的检测方法和装置
公开(公告)号：EP1130117A2
公开(公告)日：2001-09-05
申请号：EP01107486.1
申请日：1993-04-05
申请人： Abbott Laboratories
发明人： Bouma, Stanley R. , Khalil, Omar S. , Pabich, Edward K.
IPC分类号： C12Q1/68 , C12N9/12 , C07H21/04 , C12M1/38
CPC分类号： G01N21/648 , B01L7/52 , C12Q1/6813 , C12Q1/6816 , C12Q1/6825 , C12Q1/6844 , C12Q1/686 , C12Q1/6862 , G01N21/6428 , G01N2021/6484 , C12Q2565/549 , C12Q2565/537 , C12Q2563/107
摘要： An apparatus for detecting a target molecule in a specific binding assay using a fluorescence total internal reflection (TIR) element in a sealed static-volumetric reaction vessel is provided wherein the reaction vessel wall and TIR element surface are separated from one another by a distance which precludes capillary migration of an aqueous fluid.
摘要翻译：用于使用在密封静电容积反应容器中的荧光全内反射（TIR）元件中的特异性结合测定法检测靶分子提供了一种装置worin反应容器壁和TIR元件表面的距离是彼此分离，这排除了wässrige流体的毛细迁移。

2. 发明授权

EP0424633B1 Automated method and device for performing solid-phase diagnostic assay 失效
标题翻译：用于进行固相诊断分析的自动化方法和装置
公开(公告)号：EP0424633B1
公开(公告)日：1996-01-17
申请号：EP90116379.0
申请日：1990-08-27
申请人： ABBOTT LABORATORIES
发明人： Khalil, Omar S. , Huff, Denise G. , Hanna, Charles F. , Zurek, Thomas F.
IPC分类号： G01N33/543 , B01L3/00 , B01L11/00
CPC分类号： B01L3/50255 , B01L2300/069 , B01L2300/0829 , B01L2400/0406 , G01N33/54313 , G01N33/54366 , G01N33/5762 , G01N33/94 , G01N35/1002 , Y10S435/805 , Y10T436/255 , Y10T436/2575

3. 发明授权

EP0424634B1 Method and apparatus for heterogeneous chemiluminescence assay 失效
标题翻译：异构化学发光调查的方法和设备。
公开(公告)号：EP0424634B1
公开(公告)日：1995-06-14
申请号：EP90116380.8
申请日：1990-08-27
申请人： ABBOTT LABORATORIES
发明人： Khalil, Omar S. , Zurek, Thomas F. , Genger, Kevin R. , Pepe, Curtis J. , Jou, Yi-Her , Cotter, Stephen M.
IPC分类号： G01N33/52 , G01N33/58
CPC分类号： G01N33/54346 , G01N21/76 , G01N33/521 , G01N33/525 , G01N33/54366 , G01N33/54386 , G01N33/5764
摘要： Apparatus and method for performing a chemiluminescende assay involving the immobilization of a chemiluminescent reaction complex to a solid, porous element. The solid, porous element is preferably treated to provide an immobilizing interaction with the chemiluminescent reaction complex wherein the chemiluminescent reaction complex is thereby immobilized to the solid, porous element. The activating and reading of the chemiluminescent reaction are separately performed by evenly distributing a concentrated chemiluminescent activating solution to form a puddle on the surface of the porous element to which the chemiluminescent reaction complex is immobilized.

4. 发明公开

EP0424633A2 Automated method and device for performing solid-phase diagnostic assay 失效
标题翻译：用于进行固相诊断分析的自动化方法和装置。
公开(公告)号：EP0424633A2
公开(公告)日：1991-05-02
申请号：EP90116379.0
申请日：1990-08-27
申请人： ABBOTT LABORATORIES
发明人： Khalil, Omar S. , Huff, Denise G. , Hanna, Charles F. , Zurek, Thomas F.
IPC分类号： G01N33/543 , B01L3/00 , B01L11/00
CPC分类号： B01L3/50255 , B01L2300/069 , B01L2300/0829 , B01L2400/0406 , G01N33/54313 , G01N33/54366 , G01N33/5762 , G01N33/94 , G01N35/1002 , Y10S435/805 , Y10T436/255 , Y10T436/2575
摘要： A disposable device (10) suitable for performing automated solidphase diagnostic assays which employs microparticles to complex an analyte and where the microparticle complex becomes retained and immobilized on a fibrous matrix such that the presence of analyte on the microparticles can be detected by optical means. A device (10) is disclosed having a sample well (12) for receiving a sample and reagents for forming a reaction mixture, a read well (14) comprising (a) an entrance port and holding means for receiving and holding a quantity of sample and assay reagents, (b) a fibrous matrix (22) for retaining and immobilizing microparticle/analyte complexes for detection, said fibrous matrix (22) positioned below said holding means, and having an average spatial separation of fibers greater than the average diameter of said microparticles, (c) means (24) positioned below said fibrous matrix for assisting the flow of sample and assay reagents through said fibrous matrix, such as an absorbent material (24) or vacuum below said fibrous matrix, (d) means (26) for creating a substantially light-tight real surrounding the well, (e) means (28) for venting air entrapped in the absorbent material and which is displaced by fluids absorbed therein, and (f) passage means (16) communicating between the sample well and the read well whereby sample and reaction mixtures can be transferred and washed from said sample well into said read well without being contacted by any apparatus.
摘要翻译：适合于执行其采用微粒到复杂的分析物上，并且其中所述微粒复合物变得保留并固定在检测纤维基质做了微粒分析物的存在可以通过光学手段被检测到自动固相诊断测定的一次性装置。一种设备盘游离缺失具有样品孔用于接收样品和试剂形成反应混合物，读出以及包括入口端口的（a）和保持装置，用于接收和保持样品和检测试剂的量，（b）一种纤维矩阵用于保持和固定微粒/分析物复合物进行检测，所述位于下方，所述纤维基质保持装置，以及具有在纤维上比所述微粒的平均直径大的平均空间分离，（C）是指位于其下方，所述纤维基质用于辅助通过所述纤维性基质，色样和分析试剂的流：如在吸收材料或低于所述纤维基质真空，（D）用于产生基本不透光的实际围绕井，（e）用于排放在吸湿截留的空气这是通过在其中被吸收的流体移动，并且样品之间（F）的装置通道连通孔中，将读好，由此样品和反应混合物材料和所有功能能够被传输并从所述样品井到所述不受任何装置被接触以及读洗涤。

5. 发明公开

EP0424633A3 Automated method and device for performing solid-phase diagnostic assay 失效
标题翻译：用于执行固相诊断测定的自动方法和装置
公开(公告)号：EP0424633A3
公开(公告)日：1992-09-23
申请号：EP90116379.0
申请日：1990-08-27
申请人： ABBOTT LABORATORIES
发明人： Khalil, Omar S. , Huff, Denise G. , Hanna, Charles F. , Zurek, Thomas F.
IPC分类号： G01N33/543 , B01L3/00 , B01L11/00
CPC分类号： B01L3/50255 , B01L2300/069 , B01L2300/0829 , B01L2400/0406 , G01N33/54313 , G01N33/54366 , G01N33/5762 , G01N33/94 , G01N35/1002 , Y10S435/805 , Y10T436/255 , Y10T436/2575
摘要： A disposable device (10) suitable for performing automated solidphase diagnostic assays which employs microparticles to complex an analyte and where the microparticle complex becomes retained and immobilized on a fibrous matrix such that the presence of analyte on the microparticles can be detected by optical means. A device (10) is disclosed having a sample well (12) for receiving a sample and reagents for forming a reaction mixture, a read well (14) comprising (a) an entrance port and holding means for receiving and holding a quantity of sample and assay reagents, (b) a fibrous matrix (22) for retaining and immobilizing microparticle/analyte complexes for detection, said fibrous matrix (22) positioned below said holding means, and having an average spatial separation of fibers greater than the average diameter of said microparticles, (c) means (24) positioned below said fibrous matrix for assisting the flow of sample and assay reagents through said fibrous matrix, such as an absorbent material (24) or vacuum below said fibrous matrix, (d) means (26) for creating a substantially light-tight real surrounding the well, (e) means (28) for venting air entrapped in the absorbent material and which is displaced by fluids absorbed therein, and (f) passage means (16) communicating between the sample well and the read well whereby sample and reaction mixtures can be transferred and washed from said sample well into said read well without being contacted by any apparatus.

6. 发明公开

EP0424634A2 Method and apparatus for heterogeneous chemiluminescence assay 失效
标题翻译： Verfahren und Vorrichtungfürheterogene ChemilumineszierendePrüfung。
公开(公告)号：EP0424634A2
公开(公告)日：1991-05-02
申请号：EP90116380.8
申请日：1990-08-27
申请人： ABBOTT LABORATORIES
发明人： Khalil, Omar S. , Zurek, Thomas F. , Genger, Kevin R. , Pepe, Curtis J. , Jou, Yi-Her , Cotter, Stephen M.
IPC分类号： G01N33/52 , G01N33/58
CPC分类号： G01N33/54346 , G01N21/76 , G01N33/521 , G01N33/525 , G01N33/54366 , G01N33/54386 , G01N33/5764
摘要： Apparatus and method for performing a chemiluminescende assay involving the immobilization of a chemiluminescent reaction complex to a solid, porous element. The solid, porous element is preferably treated to provide an immobilizing interaction with the chemiluminescent reaction complex wherein the chemiluminescent reaction complex is thereby immobilized to the solid, porous element. The activating and reading of the chemiluminescent reaction are separately performed by evenly distributing a concentrated chemiluminescent activating solution to form a puddle on the surface of the porous element to which the chemiluminescent reaction complex is immobilized.
摘要翻译：用于进行涉及将化学发光反应复合物固定在固体多孔元件上的化学发光测定的装置和方法。优选处理固体多孔元件以提供与化学发光反应复合物的固定相互作用，其中化学发光反应复合物由此固定在固体多孔元件上。化学发光反应的激活和读取分别通过均匀分布浓缩的化学发光活化溶液在化学发光反应复合物固定的多孔元件的表面上形成一个水坑。

7. 发明公开

EP1130116A3 Method and device for detection of nucleic acid using total internal reflectance 失效
标题翻译：用于检测使用全内反射的核酸的方法和装置
公开(公告)号：EP1130116A3
公开(公告)日：2004-01-14
申请号：EP01107485.3
申请日：1993-04-05
申请人： Abbott Laboratories
发明人： Bouma, Stanley R. , Khalil, Omar S. , Pabich, Edward K.
IPC分类号： C12Q1/68 , G01N21/77
CPC分类号： G01N21/648 , B01L7/52 , C12Q1/6813 , C12Q1/6816 , C12Q1/6825 , C12Q1/6844 , C12Q1/686 , C12Q1/6862 , G01N21/6428 , G01N2021/6484 , C12Q2565/549 , C12Q2565/537 , C12Q2563/107
摘要： An apparatus and method for detecting amplified target nucleic acid is provided wherein the presence and concentration of amplified target is determined by total internal reflection (TIR) and coupling of the target to the the TIR element by scissile linkage.

8. 发明公开

EP1130117A9 Method and device for detection of nucleic acid using total internal reflectance 失效
标题翻译：为使用全内反射的核酸的检测方法和装置
公开(公告)号：EP1130117A9
公开(公告)日：2002-02-27
申请号：EP01107486.1
申请日：1993-04-05
申请人： Abbott Laboratories
发明人： Bouma, Stanley R. , Khalil, Omar S. , Pabich, Edward K.
IPC分类号： C12Q1/68 , C12N9/12 , C07H21/04 , C12M1/38
CPC分类号： G01N21/648 , B01L7/52 , C12Q1/6813 , C12Q1/6816 , C12Q1/6825 , C12Q1/6844 , C12Q1/686 , C12Q1/6862 , G01N21/6428 , G01N2021/6484 , C12Q2565/549 , C12Q2565/537 , C12Q2563/107
摘要： An apparatus for detecting a target molecule in a specific binding assay using a fluorescence total internal reflection (TIR) element in a sealed static-volumetric reaction vessel is provided wherein the reaction vessel wall and TIR element surface are separated from one another by a distance which precludes capillary migration of an aqueous fluid.

9. 发明公开

EP1130116A2 Method and device for detection of nucleic acid using total internal reflectance 失效
标题翻译：用于检测使用全内反射的核酸的方法和装置
公开(公告)号：EP1130116A2
公开(公告)日：2001-09-05
申请号：EP01107485.3
申请日：1993-04-05
申请人： Abbott Laboratories
发明人： Bouma, Stanley R. , Khalil, Omar S. , Pabich, Edward K.
IPC分类号： C12Q1/68 , C12N9/12 , C07H21/04 , C12M1/38
CPC分类号： G01N21/648 , B01L7/52 , C12Q1/6813 , C12Q1/6816 , C12Q1/6825 , C12Q1/6844 , C12Q1/686 , C12Q1/6862 , G01N21/6428 , G01N2021/6484 , C12Q2565/549 , C12Q2565/537 , C12Q2563/107
摘要： An apparatus and method for detecting amplified target nucleic acid is provided wherein the presence and concentration of amplified target is determined by total internal reflection (TIR) and coupling of the target to the the TIR element by scissile linkage.
摘要翻译：用于检测扩增的靶核酸的装置和方法被提供worin的存在和扩增的靶的浓度是确定的通过全内反射（TIR）和目标到所述TIR元件的耦合通过易切断的连接开采。

10. 发明公开

EP1130117A3 Method and device for detection of nucleic acid using total internal reflectance 失效
标题翻译：为使用全内反射的核酸的检测方法和装置
公开(公告)号：EP1130117A3
公开(公告)日：2003-12-03
申请号：EP01107486.1
申请日：1993-04-05
申请人： Abbott Laboratories
发明人： Bouma, Stanley R. , Khalil, Omar S. , Pabich, Edward K.
IPC分类号： C12Q1/68
CPC分类号： G01N21/648 , B01L7/52 , C12Q1/6813 , C12Q1/6816 , C12Q1/6825 , C12Q1/6844 , C12Q1/686 , C12Q1/6862 , G01N21/6428 , G01N2021/6484 , C12Q2565/549 , C12Q2565/537 , C12Q2563/107
摘要： An apparatus for detecting a target molecule in a specific binding assay using a fluorescence total internal reflection (TIR) element in a sealed static-volumetric reaction vessel is provided wherein the reaction vessel wall and TIR element surface are separated from one another by a distance which precludes capillary migration of an aqueous fluid.

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