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1. 发明公开

EP2494015A1 KLONIERUNG, EXPRESSION UND VERWENDUNG SAURER PHOSPHOLIPASEN 有权
标题翻译： KLONIERUNG，VERWENDUNG SAURER PHOSPHOLIPASEN表示
公开(公告)号：EP2494015A1
公开(公告)日：2012-09-05
申请号：EP10774185.2
申请日：2010-10-27
申请人： AB Enzymes GmbH
发明人： NGUYEN, Quoc, Khanh , TITZE, Kornelia , SCHWARZ, Tatiana , PALADINO, Silvia , MARSCHNER, Volker , LORENZ, Patrick
IPC分类号： C11B3/00 , C12N9/16
CPC分类号： C12N9/18 , A23D9/00 , A23D9/02 , A23J7/00 , A23K20/189 , A23V2002/00 , C11B3/00 , C11B3/003 , C12N9/16 , C12Y301/01004 , C12Y301/01005 , C12Y301/01032
摘要： The invention relates to a DNA sequence, which codes for a polypeptide having phospholipase activity essentially without lipase activity, characterized in that the DNA sequence is selected from a) DNA sequences that comprise a nucleotide sequence according to SEQ ID No: 1, b) DNA sequences that comprise the coding sequence according to SEQ ID NO: 1, c) DNA sequences that code for the protein sequence according to SEQ ID NO: 2, d) DNA sequences that are coded for with the restriction map by the pPL3940-Topo2.5 plasmid according to figure 7 and that are stored under number DSM 22741, e) DNA sequences that hybridize under stringent conditions with one of the DNA sequences according to a), b), c) or d), f) DNA sequences that are related to the DNA sequences according to a), b), c), d) or e) in the basis of the degeneration of the genetic code, and g) complementary strands to the sequences according to a) to f), wherein the DNA sequence is preferably derived from Aspergillus, and more preferably from Aspergillus fumigatus, and a polypeptide having phospholipase activity essentially without lipase activity selected from a) a polypeptide which is coded for by the coding part of a DNA sequence as defined above, b) a polypeptide having the sequence according to SEQ ID NO: a or a sequence derived therefrom, which can be obtained by substituting, adding, or deleting one or more amino acids, c) a polypeptide having a sequence that has at least 83% identity with the amino acids 1 to 299 of SEQ ID NO: 2, d) a polypeptide for which a nucleic acid sequence codes, which is hybridized under stringent conditions with (i) nucleotides 55 to 1106 of SEQ ID NO: 1, (ii) the cDNA sequence contained in nucleotides 55 to 1106 of SEQ ID NO: 1, (iii) a partial sequence of (i) or (ii) composed of at least 100 nucleotides, or (iv) a complementary strand of (i), (ii) or (iii), e) a variant of the polypeptide having SEQ ID NO: 2, comprising a substitution, deletion and/or insertion of one or more amino acids, f) allelic variants for amino acid sequences a) to e).
摘要翻译：本发明涉及编码具有基本上没有脂肪酶活性的磷脂酶活性的多肽的DNA序列，其特征在于所述DNA序列选自a）包含根据SEQ ID NO：1的核苷酸序列的DNA序列，b）DNA 包含根据SEQ ID NO：1的编码序列的序列，c）编码根据SEQ ID NO：2的蛋白质序列的DNA序列，d）由质粒pPL3940-Topo2.5编码的DNA序列，其中根据图1的限制图。 7，其以保藏号DSM 22741保藏，e）在严格条件下与根据a），b），c）或d）的DNA序列之一杂交的DNA序列，f）与DNA相关的DNA序列根据a），b），c），d）或e）由于遗传密码子的退化而产生的序列，以及g）与根据a）至f）的序列的互补链，其中所述DNA序列优选衍生自曲霉菌，更优选来自烟曲霉，以及具有基本上没有脂肪酶活性的磷脂酶活性的多肽，选自a）由上述DNA序列的编码部分编码的多肽，b）具有根据 SEQ ID NO：2或其衍生的序列，其可以通过取代，添加，缺失一个或多个氨基酸获得，c）具有与氨基酸1具有至少83％同一性的序列的多肽至299个氨基酸，d）多肽（i）SEQ ID NO：1的核苷酸55至1106，（ii）SEQ ID NO：1的核苷酸55至1106中包含的cDNA序列，（iii）的核酸序列编码，由至少100个核苷酸组成的（i）或（ii）的部分序列，或（iv）（i），（ii）或（iii）的互补链，e）具有SEQ ID NO： 2，包含一个或多个氨基酸的取代，缺失和/或插入，f）氨基酸序列a）至e）的等位基因变体。

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