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    • 4. 发明公开
    • MONOLITH-BASED PSEUDO-BIOAFFINITY PURIFICATION METHODS FOR FACTOR VIII AND APPLICATIONS THEREOF
    • AUF MONOLITH BASIERENDE PSEUDO-BIOAFFINITÄTSREINIGUNGSVERFAHRENFÜRFAKTOR VIII UND ANWENDUNGEN DAVON
    • EP2925777A1
    • 2015-10-07
    • EP13857951.1
    • 2013-11-27
    • Centre for Bioseparation Technology-VIT
    • JANAKIRAMAN, Vignesh, NarasimhanPRASANNA, Rajasekar, RajagopalKAMALANATHAN, Agamudi, SivasankaranVIJAYALAKSHMI, Mookambeswaran, Arunachalam
    • C07K14/745
    • C07K14/755C07K1/22
    • The present disclosure relates to purification of Factor VIII protein and/or its fragments from various sources by employing monolith based pseudobioaffinity purification methods. In particular, L-histidine over CIM monolith [Histidine Ligand Affinity Chromatography (HLAC)] is used for the purification of wild-type factor VIII from plasma cryoprecipitate, recombinant B-domain deleted factor VIII (rBDD-FVIII) expressed in various host systems and recombinant factor VIII light chain expressed in
      Pichia pastoris . Further, immobilized metal over CIM monolith [Immobilized metal-ion affinity chromatography (IMAC)] is employed for the purification of wild-type factor VIII, rBDD-FVIII expressed in various host systems and recombinant factor VIII heavy chain expressed in
      Pichia pastoris . The purification efficiency showcased by the purification methods of the present disclosure is far superior when compared to the presently available methods for the purification of factor VIII which lead to significantly improved results in therapeutic applications involving factor VIII.
    • 本公开涉及通过使用整体基的假离子亲和纯化方法从各种来源纯化因子VIII蛋白和/或其片段。 特别地,使用CIM整体[组氨酸配体亲和层析(HLAC)]上的L-组氨酸用于从血浆冷沉淀物中纯化野生型因子VIII,在各种宿主系统中表达的重组B结构域缺失因子VIII(rBDD-FVIII) 和重组因子VIII轻链在巴斯德毕赤酵母中表达。 此外,通过CIM整料[固定金属离子亲和层析(IMAC)]上的固定化金属被用于纯化在各种宿主系统中表达的野生型因子VIII,rBDD-FVIII和在巴斯德毕赤酵母中表达的重组因子VIII重链。 与目前可用的纯化因子VIII的方法相比,通过本公开的纯化方法显示的净化效率是显着的,其导致在涉及因子VIII的治疗应用中显着改善的结果。
    • 9. 发明公开
    • SLIDING SHEET TYPE OF VARIABLE-TEETH GEAR
    • ZAHNRAD MIT VARIABLENZÄHNENAUS VERSCHIEBBARENFLÄCHENGEBILDEN
    • EP1811205A1
    • 2007-07-25
    • EP05791902.9
    • 2005-09-21
    • Beijing Vit Mobile Technologies Co., Ltd
    • WANG, Guobin
    • F16H55/17F16H55/26F16H3/00F16H19/02F16G1/28
    • F16H3/002F16G1/28F16G5/16F16H3/423F16H3/426F16H9/24F16H55/17F16H55/171F16H55/54F16H55/56H01L2924/0002H01L2924/00
    • A variable-tooth gear with SSFT pertains to the technical fields of gearing, and variable mechanical transmissions. The variable-tooth gear is designed in accordance with "the principle of configuring variable-teeth through universally shaped SSs". The working surface is composed of a large number of thin SSs (sliding-needles) superposed on one another. The meshing tooth profiles of arbitrary shapes can be configured by the free and infinite sliding of the SSs. Since the direction of sliding of the SSs is different from the direction of forces acting thereupon, the SSs can freely deform with the current tooth profiles. The direction of the force acting upon the SS when transmitting power is perpendicular to the free-sliding direction, or the angle therebetween is within the equivalent friction angle, therefore the SS possesses the self-locking property. The tooth profile thus remains unchanged when the SSs are under stress. All the SSs form an integrally closed elastic enclosure ring, possessing the effect of "rigidity and flexibility being combined, variable-tooth being fixed". Therefore the load capacity is high, the transmission is a one-stage transmission and the transmission efficiency is high. There are only two key components, such that the structure is simple, the cost is low and the size is compact. This variable-tooth gear realizes, to the core, the concept of "continuously variable transmission via gear meshing", and can be applied to vehicles or industrial fields of high speed and large power.
    • 具有SSFT的可变齿齿轮涉及齿轮传动和可变机械变速箱的技术领域。 可变齿齿轮根据“通过普通形状的SS组态可变齿的原理”设计。 工作面由大量相互重叠的薄SS(滑针)组成。 可以通过SS的自由和无限滑动来配置任意形状的啮合齿廓。 由于SS的滑动方向与作用于其上的力的方向不同,所以SS可以随当前的齿形自由变形。 当发射动力时作用在SS上的力的方向垂直于自由滑动方向,或者它们之间的角度在等效摩擦角内,因此SS具有自锁性质。 当SS受到压力时,齿廓保持不变。 所有的SS形成一体的闭合的弹性围环,具有“刚性和柔性组合,可变齿固定”的作用。 因此,负载能力高,传输是一级传输,传输效率高。 只有两个关键部件,结构简单,成本低,尺寸紧凑。 这种可变齿齿轮实现了“通过齿轮啮合的无级变速器”的理念,可以应用于高速,大功率的车辆或工业领域。
    • 10. 发明授权
    • FISHING THROW-OFF BUOY
    • 捕鱼抛弃浮标
    • EP1553825B1
    • 2006-03-15
    • EP03756423.4
    • 2003-10-13
    • Svoboda, JiriPavlicek, Vit
    • Svoboda, JiriPavlicek, Vit
    • A01K93/00
    • A01K93/00
    • The fishing throw-off buoy composed from double float and from anchoring load of the fish-line, where the parts of float are connected with a distancing tube with a going­through fish-line. The partly the front part (11) of float (1) with rubber lock (2) for the fish­bait is located on the front part of distancing tube (3) and the rear part (12) is located sliding on the distancing tube (3). The front part (31) of which has a greater outer diameter as the rear part (32), which is provided with an end stop (33) of rear part (12) of float (1). Partly the anchoring load (5) of fish-line (4) is composed from cylinder (51) eccentrically and sliding located on the front part (31) of distancing tube (3) and in the wall of which is in the reduced diameter place and parallel to axis a cut opening (52) is provided the width of which is greater as the diameter of rear part (32) of distancing tube (3) and less as the outer diameter of front part (31) of distancing tube (3). The cylinder (51) is with help of short thimble (53) fastened to the cap ring (54) sliding on the distancing tube (3) between the rear part (12) of float (1) and between the end stop (33), the inner diameter of which is greater as the outer diameter of end stop (33).
    • 由双浮体和鱼线锚固载荷构成的抛鱼浮标,其中浮体部分与具有通过鱼线的距离管连接。 具有用于鱼饵的橡胶锁(2)的浮体(1)的部分前部(11)位于间隔管(3)的前部,并且后部(12)位于间隔管(3) )。 其前部(31)具有较大的外径作为后部(32),该后部(32)设有浮体(1)的后部(12)的末端止动件(33)。 部分鱼线(4)的锚固载荷(5)由圆柱体(51)偏心并滑动地位于间隔管(3)的前部(31)上并且其壁的直径减小的部位 并且平行于轴线设置切口(52),其宽度大于间隔管(3)的后部(32)的直径并且小于间隔管(3)的前部(31)的外径 )。 圆柱体51借助紧固到在浮体1的后部12之间和端部止挡33之间在间隔管3上滑动的盖环54的短套管53的帮助下, ,其内径与端部止动件(33)的外径相比更大。