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2. 发明公开

EP2885412A2 USE OF A MAIZE UNTRANSLATED REGION FOR TRANSGENE EXPRESSION IN PLANTS 有权转让
标题翻译： VERWENDUNG EINER UNTRANSLATIERTEN MAISREGION ZUR TRANSGENEXPRESSION BEI PFLANZEN
公开(公告)号：EP2885412A2
公开(公告)日：2015-06-24
申请号：EP13752757.8
申请日：2013-08-08
申请人： Dow AgroSciences LLC , Kumar, Sandeep , Gupta, Manju , Alabed, Diaa
发明人： KUMAR, Sandeep , GUPTA, Manju , ALABED, Diaa
IPC分类号： C12N15/82
CPC分类号： C12N15/8216 , C12N15/8218
摘要： Provided are methods, vectors and gene constructs for enhancing expression of a recombinant nucleic acid sequence in transgenic plants and plant tissues. According to the present invention, nucleic acid sequences are obtained and/or derived from the 3′ untranslated regions of genes encoding ubiquitin proteins and engineered to flank respective portions of a selected coding region of a vector. The vector construct may be introduced into plants and/or plant tissues through conventional or gene targeting procedures, resulting in enhanced expression of the selected coding region. In some embodiments, the selected coding region is a chimeric gene or gene fragment expressing one or more proteins known to impart a level of insecticidal activity to a transgenic plant and/or plant tissue.
摘要翻译：提供了用于增强转基因植物和植物组织中重组核酸序列表达的方法，载体和基因构建体。根据本发明，获得核酸序列和/或衍生自编码遍在蛋白质的基因的3'非翻译区并且被工程化成位于载体的所选编码区的相应部分。可以通过常规或基因靶向程序将载体构建体引入植物和/或植物组织中，导致选择的编码区的表达增强。在一些实施方案中，所选择的编码区是表达一种或多种已知赋予转基因植物和/或植物组织杀虫活性水平的蛋白质的嵌合基因或基因片段。

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