专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明公开

EP2087897A1 METHOD FOR PRODUCING APYROGENIC IMMUNOMODULATOR 有权转让
标题翻译： VERFAHREN ZUR HERSTELLUNG EINES APYROGENEN免疫调节剂
公开(公告)号：EP2087897A1
公开(公告)日：2009-08-12
申请号：EP07866932.2
申请日：2007-10-09
申请人： Kaplina, Elli Nikolaevna , Ladyigin, Alexandr Evgenevich , Kaplin, Valeri Yurievich
发明人： Kaplina, Elli Nikolaevna , Ladyigin, Alexandr Evgenevich , Kaplin, Valeri Yurievich
IPC分类号： A61K35/60 , A61P37/04
CPC分类号： A61K35/60 , A61K31/711
摘要： The invention relates to bioengineering, in particular to a method for producing apyrogenic immunomodulator. The inventive method consists in purifying, grinding and in homogenizing a milt roe of salmon or sturgeon fish in a aqueous citric-salt solution solution, in centrifuging a homogenate in a settling centrifuge, in removing a supernatant fluid, in homogenizing a substrate and in simultaneously adding the calculated quantity of the citric-salt aqueous solution thereto, in centrifuging and in decanting said substrate, in incubating the purified homogenate in the presence of a detergent, in adding a saturated sodium chloride solution into a reaction mass and in holding it for 2 hours, in cooling said reaction mass and in exposing it to ultrasonic treatment in baths provided with piezoelectric ceramic radiators, in separating a liquid phase by means of a sorbent agent, in eluting said sorbent agent, in microfiltering the eluate together with the liquid phase and in concentrating it, in settling a sodium desoxyribonucleate precipitate, in centrifuging it in such a way that it is separated from the liquid phase, in drying the precipitate in aseptic conditions, diluting the thus obtained powder in a sodium chloride aqueous solution and in dispensing said solution in a sterile manner. The invention makes it possible to obtain a highly-efficient and safe medicinal preparation which meets modem pharmaceutical production requirements.
摘要翻译：本发明涉及生物工程，特别涉及一种生产无创免疫调节剂的方法。本发明的方法包括在柠檬酸盐水溶液溶液中净化，研磨和匀化鲑鱼或鲟鱼的盐，在沉降离心机中离心匀浆，去除上清液，使基质均质化，同时加入计算量的柠檬酸盐水溶液，在离心分离和倾析所述底物时，在洗涤剂存在下培养纯化的匀浆液，向饱和氯化钠溶液中加入饱和氯化钠溶液并保持2 小时，在冷却所述反应物质并将其在具有压电陶瓷散热器的浴中暴露于超声波处理中，在通过吸附剂分离液相时，在洗脱所述吸附剂时，将洗脱液与液相一起微滤; 在浓缩时，在沉淀脱氧核糖核酸沉淀物的过程中，以离心分离在液相中，在无菌条件下干燥沉淀物，将如此获得的粉末稀释在氯化钠水溶液中并以无菌方式分配所述溶液。本发明可以获得满足现代医药生产要求的高效安全的药物制剂。

2. 发明公开

EP1338281A4 METHOD FOR PRODUCING AN IMMUNOTROPIC ANTIVIRAL PREPARATION 有权
标题翻译：一种用于生产IMMUNOTROPISCHEN抗病毒药剂组合物
公开(公告)号：EP1338281A4
公开(公告)日：2004-03-24
申请号：EP01930355
申请日：2001-04-24
申请人： VERITAS S R L , KAPLINA ELLI NIKOLAEVNA , LADYGINA ALEXANDRA YUREVNA , KAPLIN VALERY YUREVICH , VAINBERG MIHAIL YUREVICH
发明人： KAPLINA ELLI NIKOLAEVNA , LADYGINA ALEXANDRA YUREVNA , KAPLIN VALERY YUREVICH , VAINBERG MIHAIL YUREVICH
IPC分类号： A61K31/70 , A61K31/711 , A61K33/24 , A61K33/30 , A61K33/32 , A61K35/14 , A61K35/26 , A61K35/407 , A61K35/60 , A61P31/14 , C12N15/10 , A61K33/26 , A61K35/28 , A61K35/55 , C07H21/00 , C07H21/04
CPC分类号： A61K31/70 , A61K31/711 , A61K33/24 , A61K33/30 , A61K33/32
摘要： The invention relates to the chemical pharmaceutical industry, in particular to a method for producing an immunotropic preparation exhibiting a high antiviral activity with respect to RNA and DNA viruses. In order to produce the inventive preparation, fat-free sturgeon or salmon milt, or a calf thymus, or chicken blood, or porcine liver is pulverised and homogenised in a saline citrate solution. The produced homogenate is incubated in the presence of a detergent, a calcium chlorine solution being added at a high temperature. Afterwards, said homogenate is treated by ultrasound, a sorbent and detergent and concentrated, thereby producing a white amorphous deposit of DNA which has the following characteristics: a molecular mass ranging from 270 to 500 kD, a hyperchromatic action not less than 37 %, a protein content of less than 1 %. The DNA deposit is dried and the obtained powder is diluted in an aqueous solution of the following transition metal: Zn, Co, Mn, Sb, whereby a 1.5 % sterilised solution having a hyperchromatic action equal to or higher than 10 % is obtained.

3. 发明授权

EP2087897B1 METHOD FOR PRODUCING APYROGENIC IMMUNOMODULATOR 有权转让
标题翻译：方法用于生产免疫调节剂不致热
公开(公告)号：EP2087897B1
公开(公告)日：2011-11-16
申请号：EP07866932.2
申请日：2007-10-09
申请人： Kaplina, Elli Nikolaevna , Ladyigin, Alexandr Evgenevich , Kaplin, Valeri Yurievich
发明人： Kaplina, Elli Nikolaevna , Ladyigin, Alexandr Evgenevich , Kaplin, Valeri Yurievich
IPC分类号： A61K35/60 , A61P37/04 , A61K31/711
CPC分类号： A61K35/60 , A61K31/711
摘要： The invention relates to bioengineering, in particular to a method for producing apyrogenic immunomodulator. The inventive method consists in cleaning, grinding and in homogenising a milt roe of salmon or sturgeon fish in a citric-salt aqueous solution, in centrifuging a homogenate in a settling centrifuge, in removing a supernatant fluid, in homogenising a substrate and in simultaneously adding the calculated quantity of the citric-salt aqueous solution thereto, in centrifuging and in decanting said substrate, in incubating the cleaned homogenate in the presence of a detergent, in adding a saturated sodium chloride solution into a reaction mass and in holding it for 2 hours, in cooling said reaction mass and in exposing it to ultrasonic treatment in baths provided with piezoelectric ceramic radiators, in separating a liquid phase by means of a sorbent agent, in eluting said sorbent agent, in microfiltering the eluate together with the liquid phase and in concentrating it, in settling a sodium desoxyribonucleate precipitate, in centrifuging it in such a way that it is separated from the liquid phase, in drying the precipitate in aseptic conditions, diluting the thus obtained powder in a sodium chloride aqueous solution and in dispensing said solution in a sterile manner. The invention makes it possible to obtain a highly-efficient and safe medicinal preparation which meets modern pharmaceutical production requirements

4. 发明公开

EP2087897A4 METHOD FOR PRODUCING APYROGENIC IMMUNOMODULATOR 有权转让
标题翻译：方法用于生产免疫调节剂致热
公开(公告)号：EP2087897A4
公开(公告)日：2010-06-02
申请号：EP07866932
申请日：2007-10-09
申请人： KAPLINA ELLI NIKOLAEVNA , LADYIGIN ALEXANDR EVGENEVICH , KAPLIN VALERI YURIEVICH
发明人： KAPLINA ELLI NIKOLAEVNA , LADYIGIN ALEXANDR EVGENEVICH , KAPLIN VALERI YURIEVICH
IPC分类号： A61K35/60 , A61K31/711 , A61P37/04
CPC分类号： A61K35/60 , A61K31/711

5. 发明授权

EP1338281B1 METHOD FOR PRODUCING AN IMMUNOTROPIC ANTIVIRAL PREPARATION 有权
标题翻译：一种用于生产IMMUNOTROPISCHEN抗病毒药剂组合物
公开(公告)号：EP1338281B1
公开(公告)日：2006-03-22
申请号：EP01930355.1
申请日：2001-04-24
申请人： Kaplina, Elli Nikolaevna , Ladygina, Alexandra Yurievna , Kaplin, Valery Yurievich , Vainberg, Mihail Yurievich
发明人： Kaplina, Elli Nikolaevna , Ladygina, Alexandra Yurievna , Kaplin, Valery Yurievich , Vainberg, Mihail Yurievich
IPC分类号： C12N15/10 , A61K31/70 , A61K31/711 , A61K33/24 , A61K33/26 , A61K35/60 , A61K35/14 , A61K35/28 , A61K35/55 , C07H21/04 , C07H21/00 , A61P31/14
CPC分类号： A61K31/70 , A61K31/711 , A61K33/24 , A61K33/30 , A61K33/32
摘要： The invention relates to the chemical pharmaceutical industry, in particular to a method for producing an immunotropic preparation exhibiting a high antiviral activity with respect to RNA and DNA viruses. In order to produce the inventive preparation, fat-free sturgeon or salmon milt, or a calf thymus, or chicken blood, or porcine liver is pulverised and homogenised in a saline citrate solution. The produced homogenate is incubated in the presence of a detergent, a calcium chlorine solution being added at a high temperature. Afterwards, said homogenate is treated by ultrasound, a sorbent and detergent and concentrated, thereby producing a white amorphous deposit of DNA which has the following characteristics: a molecular mass ranging from 270 to 500 kD, a hyperchromatic action not less than 37 %, a protein content of less than 1 %. The DNA deposit is dried and the obtained powder is diluted in an aqueous solution of the following transition metal: Zn, Co, Mn, Sb, whereby a 1.5 % sterilised solution having a hyperchromatic action equal to or higher than 10 % is obtained.

6. 发明公开

EP1338281A1 METHOD FOR PRODUCING AN IMMUNOTROPIC ANTIVIRAL PREPARATION 有权
标题翻译：方法ZER HERSTELLUNG EINER IMMUNOTROPISCHEN ANTIVIRALEN ZUSAMMENSETZUNG
公开(公告)号：EP1338281A1
公开(公告)日：2003-08-27
申请号：EP01930355.1
申请日：2001-04-24
申请人： Veritas s.r.l. , Kaplina, Elli Nikolaevna , Ladygina, Alexandra Yurevna , Kaplin, Valery Yurevich , Vainberg, Mihail Yurevich
发明人： Kaplina, Elli Nikolaevna , Ladygina, Alexandra Yurevna , Kaplin, Valery Yurevich , Vainberg, Mihail Yurevich
IPC分类号： A61K31/70 , A61K35/60 , C07H21/04 , A61P31/14 , A61K31/711 , A61K35/14 , A61K35/28 , A61K35/55
CPC分类号： A61K31/70 , A61K31/711 , A61K33/24 , A61K33/30 , A61K33/32
摘要： The invention relates to the chemical pharmaceutical industry, in particular to a method for producing an immunotropic preparation exhibiting a high antiviral activity with respect to RNA and DNA viruses. In order to produce the inventive preparation, fat-free sturgeon or salmon milt, or a calf thymus, or chicken blood, or porcine liver is pulverised and homogenised in a saline citrate solution. The produced homogenate is incubated in the presence of a detergent, a calcium chlorine solution being added at a high temperature. Afterwards, said homogenate is treated by ultrasound, a sorbent and detergent and concentrated, thereby producing a white amorphous deposit of DNA which has the following characteristics: a molecular mass ranging from 270 to 500 kD, a hyperchromatic action not less than 37 %, a protein content of less than 1 %. The DNA deposit is dried and the obtained powder is diluted in an aqueous solution of the following transition metal: Zn, Co, Mn, Sb, whereby a 1.5 % sterilised solution having a hyperchromatic action equal to or higher than 10 % is obtained.
摘要翻译：本发明涉及化学制药工业，特别涉及一种相对于RNA和DNA病毒表现出高抗病毒活性的免疫调节剂的制备方法。为了生产本发明的制剂，将无脂鲟鱼或鲑鱼浆，或小牛胸腺，鸡血或猪肝在盐水柠檬酸盐溶液中粉碎和匀浆。将生产的匀浆在洗涤剂存在下温育，在高温下加入氯化钙溶液。之后，通过超声，吸附剂和洗涤剂处理所述匀浆物并浓缩，由此产生DNA的白色无定形沉积物，其具有以下特征：分子量范围为270至500kD，不超过37％的多色作用，蛋白质含量小于1％。将DNA沉积物干燥，将得到的粉末在以下过渡金属Zn，Co，Mn，Sb的水溶液中稀释，由此获得具有等于或高于10％的多色性作用的1.5％灭菌溶液。

7. 发明授权

EP0649428B1 VERFAHREN ZUR HERSTELLUNG DES NATRIUMSALZES DER DNS UND VORRICHTUNG ZUR DURCHFÜHRUNG DES VERFAHRENS 失效
标题翻译：用于钠盐DNS的用于实现工序的准备和设备
公开(公告)号：EP0649428B1
公开(公告)日：1999-11-10
申请号：EP94909281.1
申请日：1994-02-28
申请人： EFTAG ENTSTAUBUNGS- UND FÖRDERTECHNIK AG , WEINBERG, Juri Petrowitsch , KAPLINA, Elli Nikolaevna , KAPLIN, Walerij Jurjewitsch , LADIGIN, Alexander Jewgenjewitsch
发明人： WEINBERG, Juri Petrowitsch , KAPLINA, Elli Nikolajewna , KAPLIN, Walerij Jurjewitsch , LADIGIN, Alexander Jewgenjewitsch
IPC分类号： C07H1/08
CPC分类号： C12N15/1017
摘要： A process is disclosed for preparing desoxyribonucleic acid (DNA) from raw materials of animal origin. The raw materials of animal origin are crushed and homogenised in a nitrate salt solution, the homogenised material is treated at a high temperature with detergents and a concentrated sodium chloride solution, the reacted material is cooled, the cooled material is mixed with diatomite, the phases are separated by filtering and the end product is precipitated with ethyl alcohol. In order to improve the process so that it may be used on an industrial scale, ultrasound is applied for 10 to 80 minutes to the reacted material, before it is mixed with diatomite, the material being disposed in layers so that it may be uniformly and completely treated with ultrasound in predetermined conditions and at predetermined temperatures. The mixture ratio between the diatomite and the ultrasonically treated material equals 1:5 to 25 (weight in mg/volume in ml). The liquid phase separated by filtering is concentrated by pressure filtration and the precipitated DNA sodium salt is dried at 20 to 60 DEG C.

8. 发明公开

EP0649428A1 VERFAHREN ZUR HERSTELLUNG DES NATRIUMSALZES DER DNS UND VORRICHTUNG ZUR DURCHFÜHRUNG DES VERFAHRENS 失效
标题翻译：用于钠盐DNS的用于实现工序的准备和设备。
公开(公告)号：EP0649428A1
公开(公告)日：1995-04-26
申请号：EP94909281.0
申请日：1994-02-28
申请人： EFTAG ENTSTAUBUNGS- UND FÖRDERTECHNIK AG , WEINBERG, Juri Petrowitsch , KAPLINA, Elli Nikolaevna , KAPLIN, Walerij Jurjewitsch , LADIGIN, Alexander Jewgenjewitsch
发明人： WEINBERG, Juri Petrowitsch , KAPLINA, Elli Nikolajewna , KAPLIN, Walerij Jurjewitsch , LADIGIN, Alexander Jewgenjewitsch
IPC分类号： C07H21 , C12M3 , C12N15 , C12P19
CPC分类号： C12N15/1017
摘要： A process is disclosed for preparing desoxyribonucleic acid (DNA) from raw materials of animal origin. The raw materials of animal origin are crushed and homogenised in a nitrate salt solution, the homogenised material is treated at a high temperature with detergents and a concentrated sodium chloride solution, the reacted material is cooled, the cooled material is mixed with diatomite, the phases are separated by filtering and the end product is precipitated with ethyl alcohol. In order to improve the process so that it may be used on an industrial scale, ultrasound is applied for 10 to 80 minutes to the reacted material, before it is mixed with diatomite, the material being disposed in layers so that it may be uniformly and completely treated with ultrasound in predetermined conditions and at predetermined temperatures. The mixture ratio between the diatomite and the ultrasonically treated material equals 1:5 to 25 (weight in mg/volume in ml). The liquid phase separated by filtering is concentrated by pressure filtration and the precipitated DNA sodium salt is dried at 20 to 60 °C.

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式