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1. 发明公开

EP3929283A1 POLYMERASE VARIANTS 审中-公开
公开(公告)号：EP3929283A1
公开(公告)日：2021-12-29
申请号：EP21164826.6
申请日：2017-02-27
申请人： Genia Technologies, Inc.
发明人： AYER, Aruna , DALAL, Dhruti , SARVABHOWMAN, Preethi
IPC分类号： C12N9/12
摘要： The present disclosure provides variant Pol6 polymerase polypeptides, compositions comprising the Pol6 variant polypeptides, and methods for using the variant Pol6 polypeptides for determining the sequencing of nucleic acids, for example, by nanopore sequencing. The variant Pol6 polymerases possess decreased rates of dissociation of template from the polymerase-template complex, which result in increased processivity relative to the parental Pol6 polypeptides from which they are derived.

2. 发明公开

EP3733874A1 UNIDIRECTIONAL SEQUENCING USING PANDOMERS 审中-公开
公开(公告)号：EP3733874A1
公开(公告)日：2020-11-04
申请号：EP20164391.3
申请日：2013-11-07
申请人： Genia Technologies, Inc.
发明人： DAVIS, Randall , CHEN, Roger , BIBILLO, Arkadiusz , KORENBLUM, Daniel
IPC分类号： C12Q1/6869 , G01N33/487
摘要： The present invention relates to a method for nucleic acid sequencing, the method comprising: (a) providing a single stranded nucleic acid; (b) providing a plurality of probes; (c) polymerizing the plurality of probes in an order determined by hybridization of the hybridization moieties with the single stranded nucleic acid to be sequenced; (d) cleaving the cleavable groups to provide an expanded thread to be sequenced; (e) threading the expanded thread through a nanopore, wherein the gates prevent the expanded thread from threading through the nanopore in the reverse direction; and (f) detecting, with the aid of the nanopore, the loop structures of the expanded thread in the order determined by hybridization of the hybridization moieties with the single stranded nucleic acid to be sequenced, thereby sequencing the single stranded nucleic acid to be sequenced.

3. 发明授权

EP2668645B1 SYSTEM FOR COMMUNICATING INFORMATION FROM AN ARRAY OF SENSORS 有权转让
公开(公告)号：EP2668645B1
公开(公告)日：2020-09-30
申请号：EP11857055.5
申请日：2011-12-12
申请人： Genia Technologies, Inc.
发明人： CHEN, Roger
IPC分类号： G01N33/487

4. 发明公开

EP3212810A2 ALPHA-HEMOLYSIN VARIANTS WITH ALTERED CHARACTERISTICS 有权转让
标题翻译： ALPHA-溶血素变异体具有改变的特征
公开(公告)号：EP3212810A2
公开(公告)日：2017-09-06
申请号：EP15855632.4
申请日：2015-10-28
申请人： Genia Technologies, Inc.
发明人： DORWART, Michael , KORENBLUM, Daniel
IPC分类号： C12Q1/68
摘要： Described herein are variants of alpha-hemolysin having at least one mutation selected from T12R, T12K, N17R, N17K or combinations of T12 and N17 mutations. The variants in some embodiments may further comprise H144A. The α-hemolysin variants have a decreased time to thread.
摘要翻译：本文描述了具有选自T12R，T12K，N17R，N17K或T12和N17突变组合的至少一个突变的α-溶血素的变体。一些实施方案中的变体可以进一步包含H144A。 α-溶血素变体具有减少的螺纹时间。

5. 发明公开

EP3122891A1 NANOPORE-BASED SEQUENCING CHIPS USING STACKED WAFER TECHNOLOGY 有权转让
标题翻译： NANOPORENBASIERTE CHIPSEQUENZIERUNG MIT GESTAPELTER WAFERTECHNOLOGIE
公开(公告)号：EP3122891A1
公开(公告)日：2017-02-01
申请号：EP15769702.0
申请日：2015-03-11
申请人： Genia Technologies, Inc.
发明人： TIAN, Hui
IPC分类号： C12Q1/00 , C12Q1/68 , C12M1/34
CPC分类号： G01N33/48721
摘要： A nanopore based sequencing chip is disclosed. The sequencing chip comprises a first portion made from a first wafer. The first portion includes an array of nanopore cells. The first portion further includes a measurement circuit connected to one or more nanopore cells, the measurement circuit producing an output measurement signal. The first portion further includes one or more vias transmitting the output measurement signal. The sequencing chip further includes a second portion made from a second wafer, the second portion comprising one or more corresponding vias receiving the output measurement signal.
摘要翻译：公开了一种基于纳米孔的测序芯片。测序芯片包括由第一晶片制成的第一部分。第一部分包括纳米孔细胞阵列。第一部分还包括连接到一个或多个纳米孔单元的测量电路，测量电路产生输出测量信号。第一部分还包括传输输出测量信号的一个或多个通孔。排序芯片还包括由第二晶片制成的第二部分，第二部分包括接收输出测量信号的一个或多个相应的通孔。

6. 发明公开

EP3058102A2 NON-FARADAIC, CAPACITIVELY COUPLED MEASUREMENT IN A NANOPORE CELL ARRAY 有权转让
标题翻译：西班牙尼泊尔 - 泽尔马蒂克的KAPAZITIV GEKOPPELTE NICHT-FARADAY-MESSUNG
公开(公告)号：EP3058102A2
公开(公告)日：2016-08-24
申请号：EP14853691.5
申请日：2014-09-11
申请人： Genia Technologies, Inc.
发明人： CHEN, Roger J.A.
IPC分类号： C12Q1/68 , C40B20/00 , C40B20/04
CPC分类号： G01N27/44791 , G01N27/3278 , G01N27/4473 , G01N27/44743 , G01N33/48721
摘要： A method of identifying a molecule is disclosed. A molecule is drawn to a nanopore by applying a first voltage signal to a pair of electrodes during a first period, wherein the first voltage signal causes a first ionic current through the nanopore that is indicative of a property of a portion of the molecule proximate to the nanopore. The molecule is released from the nanopore by applying a second voltage signal to the pair of electrodes during a second period, wherein the second voltage signal causes a second ionic current through the nanopore. The first period and the second period are determined based at least in part on a net ionic current through the nanopore comprising the first ionic current and the second ionic current.
摘要翻译：公开了鉴定分子的方法。通过在第一时间段期间将一个第一电压信号施加到一对电极而将一个分子吸引到纳米孔，其中第一电压信号引起通过纳米孔的第一离子电流，其指示分子的一部分的性质接近于纳米孔。在第二时段期间，通过在所述一对电极施加第二电压信号，从所述纳米孔释放所述分子，其中所述第二电压信号引起通过所述纳米孔的第二离子电流。至少部分地基于通过包含第一离子电流和第二离子电流的纳米孔的净离子电流来确定第一周期和第二周期。

7. 发明公开

EP2954316A1 NANOPORE ARRAYS 审中-公开
标题翻译： NANOPORENARRAYS
公开(公告)号：EP2954316A1
公开(公告)日：2015-12-16
申请号：EP14748849.8
申请日：2014-01-28
申请人： Genia Technologies, Inc.
发明人： CHEN, Roger J.A. , FULLAGAR, David J.
IPC分类号： G01N27/447
CPC分类号： G01N33/48721
摘要： A method of analyzing molecules using a nanopore array including a plurality of cells included on a chip is disclosed. Nanopores are caused to be formed in at least a portion of the plurality of the cells. A first physical measurement of the nanopores is evaluated. It is determined whether to cause the molecules to interact with the nanopores. At least a portion of the nanopores is caused to interact with the molecules. A second physical measurement of the nanopores that indicates a property of the molecules is evaluated. It is determined whether to cause the nanopores to be reformed so that the cells may be reused to interact with additional molecules.
摘要翻译：公开了一种利用纳米孔阵列分析分子的方法，所述纳米孔阵列包括在芯片上的多个单元。导致在多个电池的至少一部分中形成纳米孔。评估纳米孔的第一物理测量。确定是否使分子与纳米孔相互作用。导致至少一部分纳米孔与分子相互作用。评估表示分子性质的纳米孔的第二物理测量。确定是否使纳米孔被重整，使得细胞可以重复使用以与另外的分子相互作用。

8. 发明公开

EP3733875A1 METHOD FOR ASSEMBLING A PROTEIN 审中-实审转让
公开(公告)号：EP3733875A1
公开(公告)日：2020-11-04
申请号：EP20164545.4
申请日：2013-11-07
申请人： Genia Technologies, Inc.
发明人： KALACHIKOV, Sergey , JU, Jingyue , MOROZOVA, Irina , DORWART, Michael
IPC分类号： C12Q1/6869 , G01N33/487 , B82Y5/00
摘要： The present invention relates a method for assembling a protein having a plurality of subunits, the method comprising: providing a plurality of first subunits; providing a plurality of second subunits, wherein the second subunits are modified with respect to the first subunits; contacting the first subunits with the second subunits in a first ratio to form a plurality of proteins having the first subunits and the second subunits, wherein the plurality of proteins have a plurality of ratios of the first subunits to the second subunits; and fractionating the plurality of proteins to enrich proteins that have a second ratio of the first subunits to the second subunits, wherein the second ratio is one second subunit per (n-1) first subunits, wherein 'n' is the number of subunits comprising the protein. Furthermore, the invention provides a nanopore comprising a plurality of subunits, wherein a polymerase is attached to one of the subunits and at least one and less than all of the subunits comprise a first purification tag.

9. 发明授权

EP2814983B1 METHODS FOR CREATING BILAYERS FOR USE WITH NANOPORE SENSORS 有权转让
公开(公告)号：EP2814983B1
公开(公告)日：2019-04-24
申请号：EP13748712.0
申请日：2013-02-15
申请人： Genia Technologies, Inc.
发明人： DAVIS, Randall , CHEN, Roger
IPC分类号： C12Q1/6869 , C12N15/11 , G01N27/327 , G01N33/487 , G01N33/543

10. 发明公开

EP3432935A1 SITE-SPECIFIC BIO-CONJUGATION METHODS AND COMPOSITIONS USEFUL FOR NANOPORE SYSTEMS 有权转让
公开(公告)号：EP3432935A1
公开(公告)日：2019-01-30
申请号：EP17714408.6
申请日：2017-03-23
申请人： Genia Technologies, Inc.
发明人： GREMYACHINSKIY, Dmitriy , TZITZILONIS, Christos
IPC分类号： A61K47/66 , C07K1/107 , C07K1/13 , C12Q1/68 , G01N33/68

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