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1. 发明公开

EP3284826A1 HUMAN TYPE 55 REPLICATION DEFECTIVE ADENOVIRUS VECTOR, METHOD FOR PREPARING SAME AND USES THEREOF 有权
标题翻译：人类55型复制缺陷型腺病毒载体，其制备方法及其用途
公开(公告)号：EP3284826A1
公开(公告)日：2018-02-21
申请号：EP15889036.8
申请日：2015-11-27
申请人： Guangzhou N Biomed Co., Ltd.
发明人： CHEN, Ling , FENG, Liqiang
IPC分类号： C12N15/861
CPC分类号： C12N15/86 , A61K39/0208 , A61K2039/5256 , C12N15/861 , C12N15/902 , C12N2710/10021 , C12N2710/10034 , C12N2710/10062 , C12N2710/10334 , C12N2710/10343 , C12N2710/10351 , C12N2710/10371 , C12N2800/22 , C12N2800/24
摘要： Provided are a human type 55 replication defective adenovirus vector, a method for preparing the same and uses thereof. The human type 55 replication defective adenovirus vector is prepared by the following method: knocking out E1 and E3 genes from Ad55, substituting the open reading frame 6 or the open reading frames 2, 3, 4, 6 and 6/7 of E4 gene in Ad55 genome with the corresponding open reading frames of Ad5 genome. In addition, an exogenous gene expression cassette may also be integrated into the E1 gene region of Ad55. Said human type 55 replication defective adenovirus vector is able to be produced in large scale in 293, PerC6 and other helper cell lines, and is able to be concentrated and purified by density gradient centrifugation. The human type 55 replication defective adenovirus vector is unable to replicate in normal human cells, thus has an attenuated phenotype, and the vector can express exogenous genes in target cells with high efficiency, thus can be used as vaccines or gene therapy vectors, as well as for drug and neutralizing antibody development and reporter-tracer system.
摘要翻译：提供了一种人类55型复制缺陷型腺病毒载体，其制备方法及其用途。通过以下方法制备人类55型复制缺陷型腺病毒载体：从Ad55敲除E1和E3基因，取代E4基因的开放阅读框6或开放阅读框2,3,4,6和6/7 Ad55基因组与相应的Ad5基因组的可读框。另外，外源基因表达盒也可整合到Ad55的E1基因区域中。所述人类55型复制缺陷型腺病毒载体能够在293，PerC6等辅助性细胞系中大量生产，并能够通过密度梯度离心浓缩和纯化。人类55型复制缺陷型腺病毒载体不能在正常人细胞中复制，因此具有减毒表型，载体可以高效率地在靶细胞中表达外源基因，因此可以用作疫苗或基因治疗载体至于药物和中和抗体的发展以及记者 - 追踪系统。

2. 发明公开

EP3284826A4 HUMAN TYPE 55 REPLICATION DEFECTIVE ADENOVIRUS VECTOR, METHOD FOR PREPARING SAME AND USES THEREOF 有权
公开(公告)号：EP3284826A4
公开(公告)日：2018-04-11
申请号：EP15889036
申请日：2015-11-27
申请人： GUANGZHOU N BIOMED CO LTD
发明人： CHEN LING , FENG LIQIANG
IPC分类号： C12N15/861
CPC分类号： C12N15/861 , C12N2710/10021 , C12N2710/10034 , C12N2710/10062
摘要： Provided are a human type 55 replication defective adenovirus vector, a method for preparing the same and uses thereof. The human type 55 replication defective adenovirus vector is prepared by the following method: knocking out E1 and E3 genes from Ad55, substituting the open reading frame 6 or the open reading frames 2, 3, 4, 6 and 6/7 of E4 gene in Ad55 genome with the corresponding open reading frames of Ad5 genome. In addition, an exogenous gene expression cassette may also be integrated into the E1 gene region of Ad55. Said human type 55 replication defective adenovirus vector is able to be produced in large scale in 293, PerC6 and other helper cell lines, and is able to be concentrated and purified by density gradient centrifugation. The human type 55 replication defective adenovirus vector is unable to replicate in normal human cells, thus has an attenuated phenotype, and the vector can express exogenous genes in target cells with high efficiency, thus can be used as vaccines or gene therapy vectors, as well as for drug and neutralizing antibody development and reporter-tracer system.

3. 发明授权

EP3284826B1 HUMAN TYPE 55 REPLICATION DEFECTIVE ADENOVIRUS VECTOR, METHOD FOR PREPARING SAME AND USES THEREOF 有权
公开(公告)号：EP3284826B1
公开(公告)日：2020-03-18
申请号：EP15889036.8
申请日：2015-11-27
申请人： Guangzhou N Biomed Co., Ltd.
发明人： CHEN, Ling , FENG, Liqiang
IPC分类号： C12N15/861

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