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1. 发明公开

EP3018203A1 Novel phytase, method for obtaining the same and use thereof 审中-公开
标题翻译： Neuartige Phytase，Verfahren zur Herstellung davon und Verwendung davon
公开(公告)号：EP3018203A1
公开(公告)日：2016-05-11
申请号：EP14192111.4
申请日：2014-11-06
申请人： Fertinagro Nutrientes, S.L.
发明人： Atares-Real, Sergio , Martin-Perez, Julia , Romero-Lopez, Joaquín , Salaet-Madorran, Ignasi , Marques-Mascarell, Ramón , Aligue, Rosa
IPC分类号： C12N9/16 , C12N15/81 , A23K20/168
CPC分类号： C12N9/16 , A23K20/189 , C12Y301/03008
摘要： The invention generally relates to novel and improved nucleic acid sequences encoding a polypeptide or protein having the enzymatic activity of a phytase and methods for effectively expressing such enzymes in a host cell.
In a first aspect, the present invention relates an isolated nucleic acid molecule i) comprising the nucleotide sequence according to SEQ ID NO: 1 encoding a protein with phytase activity or ii) having a sequence identity of at least 78 % to a nucleotide sequence according to SEQ ID NO: 1 encoding a protein with phytase activity.
The present invention also relates to an expression construct or a vector comprising the above mentioned isolated nucleic acid molecule. The present invention further relates to a method of producing a protein having the enzymatic activity of a phytase comprising the steps of:
a) introducing into a host cell a vector comprising:
i) elements regulating the expression that are functional in the host cell; and
ii) operatively linked thereto a nucleic acid molecule according to the invention;

b) cultivating the host cells obtained in step a) under conditions suitable for expression of the protein, and optionally
c) recovering the protein produced in step b) from the cell culture.
The present invention further relates to proteins obtained by the method according to the invention and the use thereof for the manufacture of additives for animal feed.
摘要翻译：本发明通常涉及编码具有植酸酶的酶活性的多肽或蛋白质的新型和改进的核酸序列，以及在宿主细胞中有效表达该酶的方法。在第一方面，本发明涉及分离的核酸分子i），其包含编码具有植酸酶活性的蛋白质的SEQ ID NO：1的核苷酸序列，或ii）与核苷酸序列具有至少78％的序列同一性的分离的核酸分子至编码具有植酸酶活性的蛋白质的SEQ ID NO：1。本发明还涉及包含上述分离的核酸分子的表达构建体或载体。本发明还涉及生产具有肌醇六磷酸酶的酶活性的蛋白质的方法，包括以下步骤：a）向宿主细胞中引入载体，所述载体包含：i）调节宿主细胞中功能的表达的元件; 和ii）可操作地连接到其上的根据本发明的核酸分子; b）在适于表达蛋白质的条件下培养在步骤a）中获得的宿主细胞，以及任选地c）从细胞培养物中回收步骤b）中产生的蛋白质。本发明还涉及通过本发明的方法获得的蛋白质及其用于制造动物饲料添加剂的用途。

2. 发明公开

EP2067760A1 PROCESS FOR OBTAINING FERTILIZERS FROM TIO2 SUBPRODUCTS, AND PRODUCTS OBTAINED IN THIS MANNER 审中-公开
标题翻译：一种获得肥料钛白副产品，并获得产品
公开(公告)号：EP2067760A1
公开(公告)日：2009-06-10
申请号：EP06807957.3
申请日：2006-09-18
申请人： Fertinagro Nutrientes, S.L.
发明人： ATARES REAL, Sergio
IPC分类号： C05D9/02 , C05C3/00
CPC分类号： C01C1/242 , C05C3/00 , C05C3/005 , C05D9/02
摘要： Process for obtaining fertilizer, and fertilizer, which comprises a stage of neutralizing a subproduct from the process of obtaining titanium dioxide with a content of metal sulphate using ammonia and the addition of water, if necessary.
摘要翻译：过程用于获得肥料，肥料和方法，其包括中和从获取的二氧化钛与使用氨和加入水金属硫酸盐的含量的处理的子产品的阶段，如果是必要的。

3. 发明公开

EP3130578A1 FERTILIZING COMPOSITION WHICH INCLUDES AN INHIBITOR OF UREASE ACTIVITY 有权转让
标题翻译： DÜNGEZUSAMMENSETZUNGMIT EINEM HEMMER DERUREASEAKTIVITÄT
公开(公告)号：EP3130578A1
公开(公告)日：2017-02-15
申请号：EP15181076.9
申请日：2015-08-14
申请人： Fertinagro Nutrientes, S.L.
发明人： ATARÉS REAL, Sergio , ROMERO LÓPEZ, Joaquín , SALAET MADORRAM, Ignasi , COLOM TOMÁS, Elena , FERRER GINES, Maria , NARANJO OLIVERO, Miguel Angel
IPC分类号： C05G3/08
CPC分类号： C05G3/08
摘要： In general the present invention relates to a fertilizing composition which includes an inhibitor of urease activity. More specifically, the invention provides a fertilizing composition with controlled availability which includes as an inhibitor of urease activity a pigment of natural origin, the pulcherrimin, an iron chelate derived from pulcherriminic acid, preferably synthetized in a natural manner by yeast of the Metschnikowia pulcherrima species.
摘要翻译：一般来说，本发明涉及包含脲酶活性抑制剂的施肥组合物。更具体地，本发明提供了具有受控可用性的受精组合物，其包含作为脲酶活性抑制剂的天然来源的色素，所述pulcherrimin，来自哌嗪酸的铁螯合物，优选以Metschnikowia pulcherrima种类的酵母以天然方式合成。

4. 发明公开

EP2743347A2 NOVEL PHYTASE, METHOD FOR OBTAINING SAME AND USE THEREOF 审中-公开
标题翻译： NEER PHYTASE，HERSTELLUNGSVERFAHRENDAFÜRUND VERWENDUNG DAVON
公开(公告)号：EP2743347A2
公开(公告)日：2014-06-18
申请号：EP11763748.8
申请日：2011-08-08
申请人： Fertinagro Nutrientes, S.L.
发明人： ALIGUE ALEMANY, Rosa , ATARES REAL, Sergio , MARQUES MASCARELL, Ramon , MARTIN PEREZ, Julia , ROMERO LOPEZ, Joaquin , SALAET MADORRAN, Ignacio
IPC分类号： C12N9/16 , C07K14/24
CPC分类号： C12N9/16 , C12N15/815 , C12Y301/03
摘要： The invention relates to an isolated Serratia odorifera gene encoding a protein or polypeptide with phytase activity, to a method for obtaining it in a yeast host cell such as Pichia pastoris for example, and to the use of the enzyme as an active ingredient in the manufacture of additives for feeds intended for animal nutrition and/or for the release of phosphate from organic manner coming from soil.
摘要翻译：本发明涉及编码具有植酸酶活性的蛋白质或多肽的分离的沙雷氏菌属基因，例如在酵母宿主细胞例如巴斯毕赤酵母中获得的方法，以及该酶在制备中作为活性成分的用途用于动物营养的饲料的添加剂和/或用于从土壤中有机地释放磷酸盐的添加剂。

5. 发明公开

EP2674411A1 METHOD FOR PRODUCING A BIOLOGICAL FERTILIZER AND BIOLOGICAL FERTILIZER THUS PRODUCED 有权转让
标题翻译：方法用于生产有机肥等方面做出有机肥料
公开(公告)号：EP2674411A1
公开(公告)日：2013-12-18
申请号：EP11715007.8
申请日：2011-02-07
申请人： Fertinagro Nutrientes, S.L.
发明人： ATARES REAL, Sergio , MARQUES MASCARELL, Ramon , MARTIN PEREZ, Julia R. , ALIGUE ALEMANY, Rosa M.
IPC分类号： C05B17/00 , C05F17/00
CPC分类号： C05F17/0045 , C05B17/00 , C05F17/0036 , Y02P20/145 , Y02W30/43 , C05D5/00 , C05D9/02 , C05F3/00 , C05F11/00
摘要： The present invention relates to a method for obtaining a biological fertilizer, as well as to the biological fertilizer obtained by means of said method. The method allows obtaining a biological fertilizer with phosphorus from the apatite mineral by means of using a mixture of degradable mineral raw materials, a protein concentrate, posidonia, fresh manure, magnesium sulfate and ferrous sulfate as degradable mineral raw materials which are a source of nitrogen, energy and minerals for the phosphate-solubilizing organisms, as well as the phosphate-solubilizing organisms themselves as a seed inoculant, with a high efficiency, on aluminum-calcium phosphate, preferably on the aluminum-calcium rock.
摘要翻译：本发明涉及一种用于获得生物肥料，以及由所述方法获得的生物肥料的方法。该方法允许通过哪些是氮源使用可降解的矿物原料，蛋白浓缩物，波西多尼亚，新鲜粪便，硫酸镁和硫酸可降解的矿物原料亚铁的混合物的装置，用于得到来自磷灰石矿物磷的生物肥料，能量和矿物质为解磷生物，以及所述解磷生物体本身作为种子接种物，以高效率，在磷酸铝钙，优选在铝 - 钙石。

6. 发明授权

EP2017242B1 METHODS FOR OBTAINING NITROGENATED FERTILIZERS AND COMPLEXES AND FERTILIZERS THUS OBTAINED 有权转让
标题翻译：工艺生产氮化肥和GOT如此复杂和肥料
公开(公告)号：EP2017242B1
公开(公告)日：2012-12-26
申请号：EP06743493.6
申请日：2006-05-11
申请人： Fertinagro Nutrientes, S.L.
发明人： ATARES REAL, Sergio
IPC分类号： C05D11/00 , C05G5/00 , C05D9/02
CPC分类号： C05G3/0058 , C05B7/00 , C05D9/02 , C05C9/005 , C05D5/00 , C05D9/00

7. 发明授权

EP2165976B1 METHOD OF COMPACTATION OF COMPOSITIONS BASED ON FERROUS SULPHATE HEPTAHYDRATE AND PRODUCT OBTAINED THEREFROM 有权转让
标题翻译：基于硫酸亚铁水合物的组合物的合成方法及由此得到的产物
公开(公告)号：EP2165976B1
公开(公告)日：2017-11-29
申请号：EP07788702.4
申请日：2007-07-02
申请人： Fertinagro Nutrientes, S.L.
发明人： ATARES REAL, Sergio
IPC分类号： C01G49/14 , C04B22/14 , C05D9/02 , C09C1/22 , C04B40/00 , C04B111/10
CPC分类号： C05D9/02 , C01G49/14 , C01P2002/52 , C01P2006/80 , C04B22/149 , C04B40/0039 , C04B2111/1081 , C09C1/22 , C04B20/023 , C04B14/10 , C04B22/142

8. 发明公开

EP3215610A1 NOVEL PHYTASE, METHOD FOR OBTAINING THE SAME AND USE THEREOF 有权转让
标题翻译：新型植物酶，获得其的方法及其用途
公开(公告)号：EP3215610A1
公开(公告)日：2017-09-13
申请号：EP15791583.6
申请日：2015-11-05
申请人： Fertinagro Nutrientes, S.L.
发明人： ATARES-REAL, Sergio , MARTIN-PEREZ, Julia , ROMERO-LOPEZ, Joaquín , SALAET-MADORRAN, Ignasi , MARQUES-MASCARELL, Ramón , ALIGUE, Rosa
IPC分类号： C12N9/16 , C12N15/81
CPC分类号： C12N9/16 , A23K20/189 , C12Y301/03008
摘要： The invention generally relates to novel and improved nucleic acid sequences encoding a polypeptide or protein having the enzymatic activity of a phytase and methods for effectively expressing such enzymes in a host cell. In a first aspect, the present invention relates an isolated nucleic acid molecule i) comprising the nucleotide sequence according to SEQ ID NO: 1 encoding a protein with phytase activity or ii) having a sequence identity of at least 78 % to a nucleotide sequence according to SEQ ID NO: 1 encoding a protein with phytase activity. The present invention also relates to an expression construct or a vector comprising the above mentioned isolated nucleic acid molecule. The present invention further relates to a method of producing a protein having the enzymatic activity of a phytase comprising the steps of: a) introducing into a host cell a vector comprising: i) elements regulating the expression that are functional in the host cell; and ii) operatively linked thereto a nucleic acid molecule according to the invention; b) cultivating the host cells obtained in step a) under conditions suitable for expression of the protein, and optionally c) recovering the protein produced in step b) from the cell culture. The present invention further relates to proteins obtained by the method according to the invention and the use thereof for the manufacture of additives for animal feed.
摘要翻译：本发明一般涉及编码具有肌醇六磷酸酶酶活性的多肽或蛋白质的新型和改进的核酸序列，以及在宿主细胞中有效表达这些酶的方法。在第一方面，本发明涉及分离的核酸分子i），其包含编码具有植酸酶活性的蛋白质的根据SEQ ID NO：1的核苷酸序列或ii）与根据SEQ ID NO：1的核苷酸序列具有至少78％的序列同一性的分离的核酸分子i）至编码具有肌醇六磷酸酶活性的蛋白质的SEQ ID NO：1。本发明还涉及包含上述分离的核酸分子的表达构建体或载体。本发明进一步涉及产生具有植酸酶酶活性的蛋白质的方法，包括以下步骤：a）将载体导入宿主细胞，所述载体包含：i）调节在宿主细胞中有功能的表达的元件; 和ii）与其有效连接的根据本发明的核酸分子; b）在适于表达蛋白质的条件下培养步骤a）中获得的宿主细胞，并且任选地c）从细胞培养物中回收步骤b）中产生的蛋白质。本发明进一步涉及通过根据本发明的方法获得的蛋白质及其用于制造动物饲料添加剂的用途。

9. 发明公开

EP3181538A1 FERTILISER COMPOSITION INCLUDING METAL IONS COMPLEXED WITH AMINO ACIDS TO IMPROVE THE SOLUBILIZATION OF PHOSPHORUS BY THE MICROORGANISMS PRESENT IN THE SOIL 有权转让
标题翻译：期金属离子料组合物用于改善磷的溶解土壤中的微生物与氨基复合放入由
公开(公告)号：EP3181538A1
公开(公告)日：2017-06-21
申请号：EP15382623.5
申请日：2015-12-15
申请人： Fertinagro Nutrientes, S.L.
发明人： ATARES REAL, Sergio , ROMERO LOPEZ, Joaquin , SALAET MADORRAN, Ignasi , COLOM TOMAS, Elena , FERRER GINES, María , NARANJO OLIVERO, Miguel Angel
IPC分类号： C05D9/02 , C05F11/08
CPC分类号： C05D9/02 , C05F11/08
摘要： The invention provides a fertiliser composition comprising divalent metal ions of copper, manganese and iron complexed with amino acids (copper, iron and manganese aminoates) to improve the ability of microorganisms present in the soil to solubilize phosphorus, usually called phosphorus solubilizing microorganisms, and thereby increase the amount of phosphorus absorbable by plants in the soil, thereby improving their growth or development.
摘要翻译：本发明提供了一种肥料组合物，包括铜，锰，铁与氨基酸（铜，铁和锰aminoates）络合的二价金属离子来提高存在于土壤中的微生物的溶解磷底栖的能力，通常称为溶磷微生物，并由此增加土壤的植物磷的吸收量，从而提高他们的成长或发展。

10. 发明公开

EP2165976A1 METHOD OF COMPACTATION OF COMPOSITIONS BASED ON FERROUS SULPHATE HEPTAHYDRATE AND PRODUCT OBTAINED THEREFROM 有权转让
标题翻译：用于压实基于铁（II）的组合物的七水合硫酸和制品的方法GOT PRODUCT
公开(公告)号：EP2165976A1
公开(公告)日：2010-03-24
申请号：EP07788702.4
申请日：2007-07-02
申请人： Fertinagro Nutrientes, S.L.
发明人： ATARES REAL, Sergio
IPC分类号： C01G49/14 , C04B22/14 , C05D9/00 , C09C1/22
CPC分类号： C05D9/02 , C01G49/14 , C01P2002/52 , C01P2006/80 , C04B22/149 , C04B40/0039 , C04B2111/1081 , C09C1/22 , C04B20/023 , C04B14/10 , C04B22/142
摘要： Confined pressure compaction procedure for ferrous sulphate heptahydrate-based compositions, a confined pressure compaction plant and a compacted product obtained by means of said procedure that is preferably indicated to reduce the Cr (VI) content in cement, and to eliminate iron sulphate deficiencies in agricultural land and crops.
摘要翻译：为硫酸亚铁基于七水合物的组合物密闭压力压实过程中，密闭压力压实植物和压实的产品由所述过程来获得并优选指示减少水泥中的铬（VI）含量，并消除在农业硫酸铁缺陷土地和庄稼。

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