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1. 发明授权

EP1461078B1 A PEPTIDE-BASED FLUORESCENCE POLARIZATION ASSAY FOR DETECTION OF ANTIBODIES TO i MYCOBACTERIUM BOVIS /i 有权
标题翻译：荧光偏振试验研究肽基础抗体牛分枝杆菌的检测
公开(公告)号：EP1461078B1
公开(公告)日：2011-12-21
申请号：EP02802505.4
申请日：2002-10-31
申请人： Diachemix LLC
发明人： SURUJBALLI, Om, P. , ROMANOWSKA, Anna , JOLLEY, Michael, E. , NASIR, Mohammad, Sarwar
IPC分类号： A61K39/00 , A61K39/04 , A61K39/40 , C12N1/12 , G01N33/53 , G01N33/531 , G01N33/533 , G01N33/566
CPC分类号： G01N33/5695 , C07K14/35 , G01N33/542
摘要： The present invention provides an assay for detection of M. bovis-infected animals. A tracer, comprising a peptide of M. bovis protein MPB70 conjugated to a fluorophore, is added to a serum sample from an animal to form a mixture. The fluorescence polarization of the mixture in then measured and compared to the fluorescence polarization of a control. The present invention further provides a tracer for use in fluorescence polarization assay to detect antibodies specific for M. bovis. The tracer comprises a peptide of M. bovis protein MPB70 conjugated to a fluorophore, such that the tracer is able to bind to antibodies specific for M. bovis to produce a detectable change in fluorescence polarization.

2. 发明公开

EP1317670A2 FLUORESCENCE POLARIZATION-BASED HOMOGENEOUS ASSAY FOR FUMONISIN DETERMINATION IN GRAINS 有权
标题翻译：荧光基于偏振的均相测试FUMONISINBESTIMMUNG谷物
公开(公告)号：EP1317670A2
公开(公告)日：2003-06-11
申请号：EP01973709.7
申请日：2001-09-10
申请人： Diachemix LLC
发明人： NASIR, Mohammad, Sarwar , JOLLEY, Michael, E.
IPC分类号： G01N33/53
CPC分类号： G01N33/542 , G01N21/6445 , G01N33/56961 , Y10S435/81 , Y10S435/975 , Y10S436/825 , Y10S530/823 , Y10T436/107497 , Y10T436/25375
摘要： A homogeneous assay for determining the fumonisin content in grains uses the technique of fluorescence polarization. A grain extract is prepared by shaking a crushed grain sample with a solvent. A mixture is prepared by combining the grain extract with a tracer and with monoclonal antibodies specific to fumonisin. The tracer is able to bind to the monoclonal antibodies to produce a detectable change in fluorescence polarization. The tracer is prepared by conjugating fumonisin to a suitable fluorophore. The fluorescence polarization of the mixture is measured. The fumonisin concentration of the mixture may be calculated using a standard curve obtained by measuring the fluorescence polarization of a series of fumonisin solutions of known concentration.

3. 发明授权

EP1881324B1 Fluorescence polarization-based homogeneous assay for deoxynivalenol determination in grains 有权
标题翻译：基于偏振的均相荧光测定对呕吐毒素的谷物的确定
公开(公告)号：EP1881324B1
公开(公告)日：2015-01-21
申请号：EP07021724.5
申请日：2001-09-10
申请人： Diachemix LLC
发明人： Nasir, Mohammad Sarwar , Jolley, Michael E.
IPC分类号： G01N33/542 , G01N33/569
CPC分类号： G01N33/542 , G01N33/56961

4. 发明公开

EP1881324A3 Fluorescence polarization-based homogeneous assay for deoxynivalenol determination in grains 有权
标题翻译：基于荧光偏振的均相测定法测定谷物中脱氧雪腐镰刀菌烯醇
公开(公告)号：EP1881324A3
公开(公告)日：2008-09-17
申请号：EP07021724.5
申请日：2001-09-10
申请人： Diachemix LLC
发明人： Nasir, Mohammad Sarwar , Jolley, Michael E.
IPC分类号： G01N33/542 , G01N33/569
CPC分类号： G01N33/542 , G01N33/56961
摘要： The invention relates to a homogeneous assay for determining the deoxynivanlenol (DON) content in grains wherein the technique of fluorescence polarization is used. A grain extract is prepared by shaking a crushed grain sample with water. A mixture is prepared by combining the grain extract with a tracer and with monoclonal antibodies specific to DON. The tracer is able to bind to the monoclonal antibodies to produce a detectable change in fluorescence polarization. The tracer is prepared by conjugating DON to a suitable fluorophore. The fluorescence polarization of the mixture is measured. The DON concentration of the mixture may be calculated using a standard curve obtained by measuring the fluorescence polarization of a series of DON solutions of known concentration.
摘要翻译：本发明涉及使用荧光偏振技术测定谷粒中脱氧木烯醇（DON）含量的均相测定法。谷物提取物通过用水摇动碎粒样品来制备。通过将谷物提取物与示踪剂和对DON特异的单克隆抗体组合来制备混合物。示踪剂能够结合单克隆抗体以产生可检测的荧光偏振变化。示踪剂通过将DON缀合至合适的荧光团而制备。测量混合物的荧光偏振。可以使用通过测量已知浓度的一系列DON溶液的荧光偏振获得的标准曲线来计算混合物的DON浓度。

5. 发明授权

EP1317670B1 FLUORESCENCE POLARIZATION-BASED HOMOGENEOUS ASSAY FOR FUMONISIN DETERMINATION IN GRAINS 有权
标题翻译：荧光基于偏振的均相测试FUMONISINBESTIMMUNG谷物
公开(公告)号：EP1317670B1
公开(公告)日：2005-12-21
申请号：EP01973709.7
申请日：2001-09-10
申请人： Diachemix LLC
发明人： NASIR, Mohammad, Sarwar , JOLLEY, Michael, E.
IPC分类号： G01N33/53
CPC分类号： G01N33/542 , G01N21/6445 , G01N33/56961 , Y10S435/81 , Y10S435/975 , Y10S436/825 , Y10S530/823 , Y10T436/107497 , Y10T436/25375
摘要： A homogeneous assay for determining the fumonisin content in grains uses the technique of fluorescence polarization. A grain extract is prepared by shaking a crushed grain sample with a solvent. A mixture is prepared by combining the grain extract with a tracer and with monoclonal antibodies specific to fumonisin. The tracer is able to bind to the monoclonal antibodies to produce a detectable change in fluorescence polarization. The tracer is prepared by conjugating fumonisin to a suitable fluorophore. The fluorescence polarization of the mixture is measured. The fumonisin concentration of the mixture may be calculated using a standard curve obtained by measuring the fluorescence polarization of a series of fumonisin solutions of known concentration.

6. 发明公开

EP1407272A4 FLUORESCENCE POLARIZATION-BASED HOMOGENOUS ASSAY FOR AFLATOXINS 有权
标题翻译：荧光偏振BERUHENDER均相检测黄曲霉毒素
公开(公告)号：EP1407272A4
公开(公告)日：2005-09-14
申请号：EP02752236
申请日：2002-07-10
申请人： DIACHEMIX LLC
发明人： NASIR MOHAMMAD SARWAR , JOLLEY MICHAEL E
IPC分类号： G01N33/533 , G01N33/542 , G01N33/58 , G01N33/92
CPC分类号： G01N33/542 , G01N33/582
摘要： A homogenous assay for determining the aflatoxin content in agricultural products uses the technique of fluorescence polarization. A solvent is used to extract aflatoxins from a sample of the agricultural product. A mixture is prepared by combining the extract with a tracer and with a monoclonal antibody specific for aflatoxin. The tracer is able to bind to the monoclonal antibody to produce a detectable change in fluorescence polarization. The tracer is prepared by conjugating an aflatoxin oxime to a suitable fluorophore. The fluorescence polarization of the mixture is measured. The aflatoxin concentration of the mixture may be calculated using a standard curve obtained by measuring the fluorescence polarization of a series of aflatoxin solutions of known concentration.

7. 发明公开

EP1461075A1 A RAPID SEROLOGICAL TEST FOR PARATUBERCULOSIS USING FLUORESCENCE POLARIZATION TECHNOLOGY 审中-公开
标题翻译：结核病PARA血清学快速测试荧光偏振技术
公开(公告)号：EP1461075A1
公开(公告)日：2004-09-29
申请号：EP02773957.2
申请日：2002-10-31
申请人： Diachemix LLC
发明人： SURUJBALLI, Om, P. , STILWELL, Kathryn, Irene
IPC分类号： A61K39/00 , A61K39/02 , A61K39/04 , A61K39/38 , A61K39/40 , A61K39/395 , C12Q1/00 , G01N33/53 , G01N33/531 , G01N33/532 , G01N33/566
CPC分类号： C07K14/345 , G01N33/5695 , G01N33/582 , G01N2469/20
摘要： The present invention provides an assay for detection of serum antibodies to M. paratuberculosis. A tracer, comprising a carbohydrate antigen isolated from M. paratuberculosis that is conjugated to a fluorophore, is added to a serum sample from an animal to form a mixture. The fluorescence polarization of the mixture is then measured. The presence of serum antibodies to M. paratuberculosis is indicated by a fluorescence polarization value of the mixture that is higher than the fluorescence polarization value of a control. The present invention further provides a tracer for use in a fluorescence polarization assay for antibodies specific for M. paratuberculosis. The tracer comprises a carbohydrate antigen isolated from M. paratuberculosis and conjugated to a fluorophore, such that the tracer is able to bind to antibodies specific for M. paratuberculosis to produce a detectable change in fluorescence polarization.

8. 发明公开

EP1881324A2 Fluorescence polarization-based homogeneous assay for deoxynivalenol determination in grains 有权
标题翻译： Polarisationsbasierter，homogener Fluoreszenz-Assay zur Bestimmung von Deoxynivalenol inKörnern
公开(公告)号：EP1881324A2
公开(公告)日：2008-01-23
申请号：EP07021724.5
申请日：2001-09-10
申请人： Diachemix LLC
发明人： Nasir, Mohammad Sarwar , Jolley, Michael E.
IPC分类号： G01N33/542 , G01N33/569
CPC分类号： G01N33/542 , G01N33/56961
摘要： The invention relates to a homogeneous assay for determining the deoxynivanlenol (DON) content in grains wherein the technique of fluorescence polarization is used. A grain extract is prepared by shaking a crushed grain sample with water. A mixture is prepared by combining the grain extract with a tracer and with monoclonal antibodies specific to DON. The tracer is able to bind to the monoclonal antibodies to produce a detectable change in fluorescence polarization. The tracer is prepared by conjugating DON to a suitable fluorophore. The fluorescence polarization of the mixture is measured. The DON concentration of the mixture may be calculated using a standard curve obtained by measuring the fluorescence polarization of a series of DON solutions of known concentration.
摘要翻译：本发明涉及用于测定其中使用荧光偏振技术的晶粒中脱氧鸟苷醇（DON）含量的均匀测定法。通过用水摇动破碎的谷物样品制备谷物提取物。通过将谷物提取物与示踪剂和特异于DON的单克隆抗体组合来制备混合物。示踪剂能够结合单克隆抗体以产生可检测的荧光偏振变化。通过将DON与合适的荧光团缀合来制备示踪剂。测量混合物的荧光偏振。可以使用通过测量已知浓度的一系列DON溶液的荧光偏振获得的标准曲线来计算混合物的DON浓度。

9. 发明授权

EP1320755B1 FLUORESCENCE POLARIZATION-BASED HOMOGENEOUS ASSAY FOR DEOXYNIVALENOL DETERMINATION IN GRAINS 有权
标题翻译：均相荧光偏振分析FOR呕吐毒素谷物
公开(公告)号：EP1320755B1
公开(公告)日：2007-11-14
申请号：EP01975741.8
申请日：2001-09-10
申请人： Diachemix LLC
发明人： NASIR, Mohammad, Sarwar , JOLLEY, Michael, E.
IPC分类号： G01N33/569
CPC分类号： G01N33/542 , G01N33/56961
摘要： a homogeneous assay for determining the deoxynivanlenol (DON) content in grains uses the technique of fluorescence polarization. A grain extract is prepared by shaking a crushed grain sample with water. A mixture is prepared by combining the grain extract with a tracer and with monoclonal antibodies specific to DON. The tracer is able to bind to the monoclonal antibodies to produce a detectable change in fluorescence polarization. The tracer is prepared by conjugating DON to a suitable fluorophore. The fluorescence polarization of the mixture is measured. The DON concentration of the mixture may be calculated using a standard curve obtained by measuring the fluorescence polarization of a series of DON solutions of known concentration.

10. 发明公开

EP1461078A4 A PEPTIDE-BASED FLUORESCENCE POLARIZATION ASSAY FOR DETECTION OF ANTIBODIES TO i MYCOBACTERIUM BOVIS /i 有权
标题翻译：荧光偏振试验研究肽基础抗体牛分枝杆菌的检测
公开(公告)号：EP1461078A4
公开(公告)日：2005-08-24
申请号：EP02802505
申请日：2002-10-31
申请人： DIACHEMIX LLC
发明人： SURUJBALLI OM P , ROMANOWSKA ANNA , JOLLEY MICHAEL E , NASIR MOHAMMAD SARWAR
IPC分类号： C07K14/35 , G01N33/542 , G01N33/569 , A61K39/00 , A61K39/04 , A61K39/40 , C12N1/12 , G01N33/53 , G01N33/531 , G01N33/533 , G01N33/566
CPC分类号： G01N33/5695 , C07K14/35 , G01N33/542
摘要： The present invention provides an assay for detection of M. bovis-infected animals. A tracer, comprising a peptide of M. bovis protein MPB70 conjugated to a fluorophore, is added to a serum sample from an animal to form a mixture. The fluorescence polarization of the mixture in then measured and compared to the fluorescence polarization of a control. The present invention further provides a tracer for use in fluorescence polarization assay to detect antibodies specific for M. bovis. The tracer comprises a peptide of M. bovis protein MPB70 conjugated to a fluorophore, such that the tracer is able to bind to antibodies specific for M. bovis to produce a detectable change in fluorescence polarization.

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